Quantification of hTERT mRNA and Telomerase Activity in Bladder Washings of Patients with Recurrent Urothelial Cell Carcinomas

Kok, Jacques B. de; Balken, Michael R. van; Roelofs, Rian; Aarssen, Yvonne A.W.G. van; Swinkels, Dorine W.; Gunnewiek, Jacqueline M. T. Klein

doi:10.1093/clinchem/46.12.2003

Cited by 20 publications

(7 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, a significant association of telomerase activity with hTERT expression has been already previously shown [ 26 , 34 ]. Such a higher expression in bladder washing fluids is in agreement with other previous findings [ 22 - 24 , 26 , 34 - 38 ].…”

Section: Discussionsupporting

confidence: 93%

Expression and biological-clinical significance of hTR, hTERT and CKS2 in washing fluids of patients with bladder cancer

et al. 2010

View full text Add to dashboard Cite

Backgroundat present, pathogenesis of bladder cancer (BC) has not been fully elucidated. Aim of this study is to investigate the role of human telomerase RNA (hTR), human telomerase reverse transcriptase (hTERT) and CDC28 protein kinase regulatory subunit 2 (CKS2) in bladder carcinogenesis and their possible clinical significance;Methodsthe transcript levels of hTR, hTERT and CKS2 were quantified by Real time reverse transcriptase chain reaction in exfoliated cells from bladder washings of 36 patients with BC and 58 controls. The statistical significance of differences between BC bearing patients and control groups, in the general as well as in the stratified analysis (superficial or invasive BC), was assessed by Student's t test. Non parametric Receiver Operating Characteristics analysis (ROC) was performed to ascertain the accuracy of study variables to discriminate between BC and controls. The clinical value of concomitant examination of hTR, hTERT and CKS2 was evaluated by logistic regression analysis;Resultsa significant decrease in hTR and a significant increase in hTERT or CKS2 gene expression were found between BC bearing patients and controls, as well as in the subgroups analysis. The area under the curve (AUC) indicated an average discrimination power for the three genes, both in the general and subgroups analysis, when singularly considered. The ability to significantly discriminate between superficial and invasive BC was observed only for hTR transcript levels. A combined model including hTR and CKS2 was the best one in BC diagnosis;Conclusionsour results, obtained from a sample set particularly rich of exfoliated cells, provide further molecular evidence on the involvement of hTR, hTERT and CKS2 gene expression in BC carcinogenesis. In particular, while hTERT and CKS2 gene expression seems to have a major involvement in the early stages of the disease, hTR gene expression, seems to be more involved in progression. In addition, our findings suggest that the studied genes have a clinical role in discriminating between BC and controls in the general as well as in the stratified analysis, when singularly considered. A combined model improved over the single marker BC diagnosis.

show abstract

Section: Discussionsupporting

confidence: 93%

Expression and biological-clinical significance of hTR, hTERT and CKS2 in washing fluids of patients with bladder cancer

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Given the results of the histological studies, we decided to study some molecular markers in order to know if there was an involution state or a transformed one. We studied the genes IGF-I and IGF-BP5, whose expression is modified in prostate involutions states after castration (Nickerson et al, 1998) and rtert, the catalytic portion of the telomerase, which is increased on transformed cells (de Kok et al, 2000). ␤-Actin was used as an internal control.…”

Section: Rt-pcrmentioning

confidence: 99%

Morphological changes and oxidative stress in rat prostate exposed to a non-carcinogenic dose of cadmium

et al. 2004

View full text Add to dashboard Cite

“…cytology yields more tumor cells in the sample and is more sensitive in identifying cancer, especially high-grade tumors, [9][10][11] but it also has a higher falsepositive rate than voided urine cytology.…”

mentioning

confidence: 99%

Surveillance for Recurrent Bladder Cancer Using a Point-of-Care Proteomic Assay

Grossman

Soloway

Messing

et al. 2006

JAMA

239

View full text Add to dashboard Cite

show abstract

Quantification of hTERT mRNA and Telomerase Activity in Bladder Washings of Patients with Recurrent Urothelial Cell Carcinomas

Cited by 20 publications

References 7 publications

Expression and biological-clinical significance of hTR, hTERT and CKS2 in washing fluids of patients with bladder cancer

Expression and biological-clinical significance of hTR, hTERT and CKS2 in washing fluids of patients with bladder cancer

Morphological changes and oxidative stress in rat prostate exposed to a non-carcinogenic dose of cadmium

Surveillance for Recurrent Bladder Cancer Using a Point-of-Care Proteomic Assay

Contact Info

Product

Resources

About