Quantification of human angiotensinogen by a novel sandwich ELISA

Suzaki, Yuki; Ozawa, Yuri; Kobori, Hiroyuki

doi:10.1016/j.peptides.2006.05.006

Cited by 20 publications

(16 citation statements)

References 15 publications

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“…Recently, an ELISA that used antiangiotensinogen antibody was reported (37,38). In contrast to our measurement that detects intact angiotensinogen only, the ELISA detects intact angiotensinogen as well as its inactive residue, the des-angiotensin I-angiotensinogen (des-AngI-Ao), cleaved by renin (39).…”

Section: Discussionmentioning

confidence: 90%

Urinary Angiotensinogen as a Marker of Intrarenal Angiotensin II Activity Associated with Deterioration of Renal Function in Patients with Chronic Kidney Disease

Yamamoto

Nakagawa

Suzuki

et al. 2007

Journal of the American Society of Nephrology

178

185

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In chronic kidney disease (CKD), enhanced intrarenal angiotensin II (AngII) is involved in deterioration of renal function, but it is difficult to measure it. For assessment of the potential of urinary angiotensinogen as a marker of intrarenal AngII activity, the correlation of plasma and urinary renin-angiotensin system components, including angiotensinogen, with deterioration of renal function was investigated in 80 patients who had CKD and were not treated with AngII blocking agents. Changes that were induced by 14 d of losartan treatment (25 mg/d) were also measured in 28 patients. Angiotensinogen was measured by RIA of AngI after incubation with renin. Urinary angiotensinogen levels were greater in patients with low estimated GFR and elevated urinary protein and type IV collagen and correlated with renal AngII and type I collagen immunostaining intensities. The risk for deterioration of renal function (i.e., estimated GFR decline of >2.5 ml/min per yr) during a mean follow-up period of 23 mo (maximum 43 mo) was associated with urinary angiotensinogen of >3.0 nmol AngI equivalent per 1 g of urinary creatinine (AngI Eq/g Cre) at enrollment (hazard ratio 3.52). The event-free survival for deterioration of renal function was better in patients with urinary angiotensinogen <3.0 nmol AngI Eq/g Cre than those >3.0 nmol AngI Eq/g Cre. Losartan reduced urinary and plasma angiotensinogen, urinary protein and type IV collagen, and systolic BP, despite concomitant increases in plasma renin and AngII. These data suggest that urinary angiotensinogen is a potentially suitable marker of intrarenal AngII activity associated with increased risk for deterioration of renal function in patients with CKD.

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Section: Discussionmentioning

confidence: 90%

Urinary Angiotensinogen as a Marker of Intrarenal Angiotensin II Activity Associated with Deterioration of Renal Function in Patients with Chronic Kidney Disease

Yamamoto

Nakagawa

Suzuki

et al. 2007

Journal of the American Society of Nephrology

178

185

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“…Above 2 studies used indirect measurements to assess the urinary angiotensinogen levels because simple and accurate methods to measure human angiotensinogen directly are unavailable at this time. Recently, two independent groups have developed enzyme linked immunosorbent assay system to measure angiotensinogen directly [26,54]. Outcomes of clinical studies using the direct measurement of human angiotensinogen are expected in the near future.…”

Section: Discussionmentioning

confidence: 99%

“…The primary antibody against human angiotensinogen was raised by Zymed as a custom service and was characterized previously [26] and the concentration for immunohistochemistry was 1:6,000.…”

Section: Immunohistochemistry Of Angiotensinogenmentioning

confidence: 99%

Enhanced intrarenal oxidative stress and angiotensinogen in IgA nephropathy patients

Kobori

Katsurada

Ozawa

et al. 2007

Biochemical and Biophysical Research Communications

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This study was performed to determine whether immunoreactivity of intrarenal hemeoxygenase-1 and angiotensinogen are increased in IgA nephropathy (IgAN) patients. Hemeoxygenase-1 and angiotensinogen immunoreactivity were determined by immunohistochemistry robot system in renal specimens from 39 patients with IgAN. Normal portions of surgically resected kidney served as controls. IgAN patients showed moderate proteinuria (1.1+/−0.2 g/day); however, the control group did not show any proteinuria. Immunoreactivity of intrarenal hemeoxygenase-1 and angiotensinogen in IgAN were significantly increased compared to normal kidneys (2.42+/−0.42 vs 1.00+/−0.26 for hemeoxygenase-1 and 4.05+/−0.40 vs 1.00+/−0.21 for angiotensinogen, arbitrary unit). Even though these IgAN patients did not show massive renal damage, hemeoxygenase-1 and angiotensinogen immunoreactivity were increased in these patients at this time point. These data suggest that activated intrarenal reactive oxygen species-angiotensinogen axis plays some roles in development of IgAN at the early stage and will provide supportive foundation of effectiveness of the renin-angiotensin system blockade in IgAN.

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“…2). Recently, two independent groups have developed an enzyme-linked immunosorbent assay system to measure angiotensinogen directly (Lantelme et al, 2005;Suzaki et al, 2006a). Outcomes of clinical studies are expected in the near future.…”

Section: A Angiotensinogenmentioning

confidence: 99%

The Intrarenal Renin-Angiotensin System: From Physiology to the Pathobiology of Hypertension and Kidney Disease

et al. 2007

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Quantification of human angiotensinogen by a novel sandwich ELISA

Cited by 20 publications

References 15 publications

Urinary Angiotensinogen as a Marker of Intrarenal Angiotensin II Activity Associated with Deterioration of Renal Function in Patients with Chronic Kidney Disease

Urinary Angiotensinogen as a Marker of Intrarenal Angiotensin II Activity Associated with Deterioration of Renal Function in Patients with Chronic Kidney Disease

Enhanced intrarenal oxidative stress and angiotensinogen in IgA nephropathy patients

The Intrarenal Renin-Angiotensin System: From Physiology to the Pathobiology of Hypertension and Kidney Disease

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