Quantification of Human Fecal Bifidobacterium Species by Use of Quantitative Real-Time PCR Analysis Targeting the
            <i>groEL</i>
            Gene

Junick, Jana; Blaut, Michaël

doi:10.1128/aem.07749-11

Cited by 105 publications

(102 citation statements)

References 56 publications

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“…Analysis of cecal content Cecal bacteria were quantified using qPCR targeting the singlecopy gene groEL 23,24 as described previously. 17 Cecal concentrations of acetate, propionate, and butyrate were determined by gas chromatography and polyamines by high-performance liquid chromatography.…”

Section: Morphometrical Analysismentioning

confidence: 99%

Association of germ-free mice with a simplified human intestinal microbiota results in a shortened intestine

et al. 2014

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Section: Morphometrical Analysismentioning

confidence: 99%

Association of germ-free mice with a simplified human intestinal microbiota results in a shortened intestine

et al. 2014

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“…Averages and standard errors were calculated on the basis of the three biological repetitions. The standard curves for the tuf genes of B. longum NCC2705 and E. rectale ATCC 33656 were constructed according to a method previously described (42). Briefly, this method involves a PCR assay (see Table S2 in the supplemental material), using a TProfessional Basic gradient thermocycler (Biometra, Montreal, QC, Canada), with primers binding to sequences up-and downstream of the qPCR primerbinding sites of the tuf gene to obtain PCR amplicons containing the target sequence, followed by a subsequent cleanup (QIAquick PCR purification kit; Qiagen) of the amplification product to create qPCR standards.…”

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confidence: 99%

Mutual Cross-Feeding Interactions between Bifidobacterium longum subsp. longum NCC2705 and Eubacterium rectale ATCC 33656 Explain the Bifidogenic and Butyrogenic Effects of Arabinoxylan Oligosaccharides

Rivière

Gagnon

Weckx

et al. 2015

Appl Environ Microbiol

181

140

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Arabinoxylan oligosaccharides (AXOS) are a promising class of prebiotics that have the potential to stimulate the growth of bifidobacteria and the production of butyrate in the human colon, known as the bifidogenic and butyrogenic effects, respectively. Although these dual effects of AXOS are considered beneficial for human health, their underlying mechanisms are still far from being understood. Therefore, this study investigated the metabolic interactions between Bifidobacterium longum subsp. longum NCC2705 (B. longum NCC2705), an acetate producer and arabinose substituent degrader of AXOS, and Eubacterium rectale ATCC 33656, an acetate-converting butyrate producer. Both strains belong to prevalent species of the human colon microbiota. The strains were grown on AXOS during mono-and coculture fermentations, and their growth, AXOS consumption, metabolite production, and expression of key genes were monitored. The results showed that the growth of both strains and gene expression in both strains were affected by cocultivation and that these effects could be linked to changes in carbohydrate consumption and concomitant metabolite production. The consumption of the arabinose substituents of AXOS by B. longum NCC2705 with the concomitant production of acetate allowed E. rectale ATCC 33656 to produce butyrate (by means of a butyryl coenzyme A [CoA]:acetate CoA-transferase), explaining the butyrogenic effect of AXOS. Eubacterium rectale ATCC 33656 released xylose from the AXOS substrate, which favored the B. longum NCC2705 production of acetate, explaining the bifidogenic effect of AXOS. Hence, those interactions represent mutual cross-feeding mechanisms that favor the coexistence of bifidobacterial strains and butyrate producers in the same ecological niche. In conclusion, this study provides new insights into the bifidogenic and butyrogenic effects of AXOS. F ermentation in the human colon is carried out by trillions of bacteria that contribute not only to health and well-being but also to disease (1-4). For instance, a decrease in the relative abundance of bifidobacteria in the human colon has been associated with antibiotic-associated diarrhea, irritable bowel syndrome, inflammatory bowel disease, allergies, and regressive autism (5, 6). Although it is currently not yet clear whether changes in microbial composition are a cause or a consequence of these disorders, there are indications that metabolites, in particular, the short-chain fatty acids (SCFAs), such as acetate, butyrate, and propionate, that are produced during fermentation in the colon play an important role in intestinal homeostasis (3, 7). Among the SCFAs produced in the human colon, butyrate has drawn the most attention, as it is an essential energy source for the colon epithelial cells and has a protective effect against inflammatory bowel disease and colon cancer (3,8,9). Most butyrate producers belong to the Firmicutes phylum and use a butyryl coenzyme A (CoA):acetate CoA-transferase in the final step of butyrate biosynthesis, which involves the n...

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“…(17,23,38), F-RNA bacteriophage subgroups (21), Rhodococcus coprophilus (41), Bacteroides group (8, 39), Bifidobacterium spp. (3,6,7,11,25,31), and biochemical fingerprinting of bacterial populations (32).…”

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New Molecular Quantitative PCR Assay for Detection of Host-Specific Bifidobacteriaceae Suitable for Microbial Source Tracking

Gómez-Doñate

Muniesa

Blanch

2012

Appl Environ Microbiol

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Bifidobacterium spp. belong to the commensal intestinal microbiota of warm-blooded animals. Some strains of Bifidobacterium show host specificity and have thus been proposed as host-specific targets to determine the origin of fecal pollution. Most strains have been used in microbial-source-tracking (MST) studies based on culture-dependent methods. Although some of these approaches have proved very useful, the low prevalence of culturable Bifidobacterium strains in the environment means that molecular culture-independent procedures could provide practical applications for MST. Reported here is a set of common primers and four Bifidobacterium sp. host-associated (human, cattle, pig, and poultry) probes for quantitative-PCR (qPCR) assessment of fecal source tracking. This set was tested using 25 water samples of diverse origin: urban sewage samples, wastewater from four abattoirs (porcine, bovine, and poultry), and water from a river with a low pollution load. The selected sequences showed a high degree of host specificity. There were no cross-reactions between the qPCR assays specific for each origin and samples from different fecal origins. On the basis of the findings, it was concluded that the host-specific qPCRs are sufficiently robust to be applied in environmental MST studies. Waterborne diseases transmitted by the fecal-oral route make a significant contribution to the burden of disease worldwide (49). Consequently, fecal pollution in water is a global public health concern. This pollution has several origins: direct discharge of fecal waste or raw wastewater, secondary effluents from wastewater treatment works, combined sewer and sanitary overflows, animal husbandry activities, wastewater from abattoirs and the meat industry, and wildlife (47). Source-tracking methods allow the origin of fecal pollution in a particular body of water to be determined (19). The identification of fecal-pollution sources accompanied by appropriate water resource management policies could contribute to improving the microbial quality of water (9,22).Several chemical and microbiological methods have been proposed for tracking the origin of fecal pollution in water (10). Unfortunately, the most commonly used fecal indicators of microorganisms, such as fecal coliforms (FC), Escherichia coli, enterococci, and sulfite-reducing clostridia (SRC), are found in both human and animal feces, and thus, they are of limited use in identifying the origin of waste. Alternative microbial indicators may provide information about the presence and origin of fecal pollution. They include antibiotic-resistant enterococcus phenotypes (18), bacteriophages of Bacteroides spp. (17,23,38), F-RNA bacteriophage subgroups (21), Rhodococcus coprophilus (41), Bacteroides group (8, 39), Bifidobacterium spp. (3,6,7,11,25,31), and biochemical fingerprinting of bacterial populations (32).Bifidobacterium spp. were first proposed as microbial-sourcetracking (MST) indicators in 1983, when sorbitol fermentation was related to a possible human source (33). Bifidobacte...

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Quantification of Human Fecal Bifidobacterium Species by Use of Quantitative Real-Time PCR Analysis Targeting the groEL Gene

Cited by 105 publications

References 56 publications

Association of germ-free mice with a simplified human intestinal microbiota results in a shortened intestine

Association of germ-free mice with a simplified human intestinal microbiota results in a shortened intestine

Mutual Cross-Feeding Interactions between Bifidobacterium longum subsp. longum NCC2705 and Eubacterium rectale ATCC 33656 Explain the Bifidogenic and Butyrogenic Effects of Arabinoxylan Oligosaccharides

New Molecular Quantitative PCR Assay for Detection of Host-Specific Bifidobacteriaceae Suitable for Microbial Source Tracking

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