Quantification of Indoxyl Sulphate in the Spent Dialysate Using Fluorescence Spectra

Holmar, Jana; Arund, Jürgen; Uhlin, Fredrik; Tanner, Risto; Fridolin, Ivo

doi:10.1007/978-3-642-21683-1_11

Cited by 7 publications

(6 citation statements)

References 10 publications

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“…Unfortunately, the total contribution of IS to the total absorbance is so negligible, from 0.3% at 254 nm up to 2.5% at 280 nm ( Figure 4 ), that it seems impossible to follow the removal of this marker and protein bound solutes in total by means of online UV monitoring of the dialysis. A novel promising method has been proposed lately for monitoring the removal of IS, a known protein-bound solute and uremic toxin, with a low removal ratio, utilizing fluorescence [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Do Only Small Uremic Toxins, Chromophores, Contribute to the Online Dialysis Dose Monitoring by UV Absorbance?

Arund

Tanner

Uhlin

et al. 2012

Toxins

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The aim of this work was to evaluate the contributions of the main chromophores to the total UV absorbance of the spent dialysate and to assess removal dynamics of these solutes during optical on-line dialysis dose monitoring. High performance chromatography was used to separate and quantify UV-absorbing solutes in the spent dialysate sampled at the start and at the end of dialysis sessions. Chromatograms were monitored at 210, 254 and 280 nm routinely and full absorption spectra were registered between 200 and 400 nm. Nearly 95% of UV absorbance originates from solutes with high removal ratio, such as uric acid. The contributions of different solute groups vary at different wavelengths and there are dynamical changes in contributions during the single dialysis session. However, large standard deviation of the average contribution values within a series of sessions indicates remarkable differences between individual treatments. A noteworthy contribution of Paracetamol and its metabolites to the total UV absorbance was determined at all three wavelengths. Contribution of slowly dialyzed uremic solutes, such as indoxyl sulfate, was negligible.

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Section: Discussionmentioning

confidence: 99%

Do Only Small Uremic Toxins, Chromophores, Contribute to the Online Dialysis Dose Monitoring by UV Absorbance?

Arund

Tanner

Uhlin

et al. 2012

Toxins

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“…UV-VIS-NIR spectrophotometer (V-570, JASCO Corp., Tokyo, Japan) was used in Sweden and UV-3600, SHIMADZU, Japan was used in Tallinn. The wavelengths were chosen on the basis of earlier spent dialysate measurements [ 23 , 24 ].…”

Section: Methodsmentioning

confidence: 99%

An Optical Method for Serum Calcium and Phosphorus Level Assessment during Hemodialysis

Holmar

Uhlin

Fernström

et al. 2015

Toxins

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Survival among hemodialysis patients is disturbingly low, partly because vascular calcification (VC) and cardiovascular disease are highly prevalent. Elevated serum phosphorus (P) and calcium (Ca) levels play an essential role in the formation of VC events. The purpose of the current study was to reveal optical monitoring possibilities of serum P and Ca values during dialysis. Twenty-eight patients from Tallinn (Estonia) and Linköping (Sweden) were included in the study. The serum levels of Ca and P on the basis of optical information, i.e., absorbance and fluorescence of the spent dialysate (optical method) were assessed. Obtained levels were compared in means and SD. The mean serum level of Ca was 2.54 ± 0.21 and 2.53 ± 0.19 mmol/L; P levels varied between 1.08 ± 0.51 and 1.08 ± 0.48 mmol/L, measured in the laboratory and estimated by the optical method respectively. The levels achieved were not significantly different (p = 0.5). The Bland-Altman 95% limits of agreement between the two methods varied from −0.19 to 0.19 for Ca and from −0.37 to 0.37 in the case of P. In conclusion, optical monitoring of the spent dialysate for assessing the serum levels of Ca and P during dialysis seems to be feasible and could offer valuable and continuous information to medical staff.

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“…The values differ at most by about 20% to both directions. In addition to UV light absorption, also fluorescence has been used for quantitation of removal of uraemic toxins by dialysis (Holmar et al 2011;Holmar et al 2013;Arund et al 2016).…”

Section: Uv Light Absorptionmentioning

confidence: 99%

Continuing education: online monitoring of haemodialysis dose

Vartia

2018

Journal of Renal Care

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Quantification of Indoxyl Sulphate in the Spent Dialysate Using Fluorescence Spectra

Cited by 7 publications

References 10 publications

Do Only Small Uremic Toxins, Chromophores, Contribute to the Online Dialysis Dose Monitoring by UV Absorbance?

Do Only Small Uremic Toxins, Chromophores, Contribute to the Online Dialysis Dose Monitoring by UV Absorbance?

An Optical Method for Serum Calcium and Phosphorus Level Assessment during Hemodialysis

Continuing education: online monitoring of haemodialysis dose

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