2016
DOI: 10.1016/j.jchromb.2015.07.029
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Quantification of lipoic acid from skin samples by HPLC using ultraviolet, electrochemical and evaporative light scattering detectors

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Cited by 26 publications
(16 citation statements)
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References 31 publications
(36 reference statements)
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“…CA recoveries were >85% for all drug concentrations and skin matrices evaluated. Our recovery results are above those of most of the scientific literature reports for experiments on drug extraction from the skin (Campos, Praça, & Bentley, ; Vávrová, Lorencová, Klimentová, Novotný, & Hrabálek, ).…”
Section: Resultssupporting
confidence: 55%
“…CA recoveries were >85% for all drug concentrations and skin matrices evaluated. Our recovery results are above those of most of the scientific literature reports for experiments on drug extraction from the skin (Campos, Praça, & Bentley, ; Vávrová, Lorencová, Klimentová, Novotný, & Hrabálek, ).…”
Section: Resultssupporting
confidence: 55%
“…All recovery values obtained from the different skin fractions were >85%. In some experiments, the recovery was around 100%, which eliminates the need to use a correction factor following permeation experiments (Campos, Praça, & Bentley, ; De Paula, Martins, & Bentley, ; Vávrová, Lorencová, Klimentová, Novotný, & Hrabálek, ).…”
Section: Resultsmentioning
confidence: 99%
“…Thus, herein the use of human skin was avoided and the purpose of this study was to provide a comparison of various animal skin types that could serve as skin membrane models for in-vitro penetration studies. The use of porcine ear skin, hairless mouse skin and shed snake skin has been widely studied as animal model membranes to replace human skin (Vecchia and Bunge, 2005;Itoh et al, 1990a;Itoh et al, 1990b;Rigg and Barry, 1990) as well as to evaluate skin permeation enhancers (Campos et al, 2016;Petrilli et al, 2013;Praça et al, 2012;Baby et al, 2008;Lopes et al, 2007;Nunes et al, 2005;Nicolazzo et al, 2003;Hirvonen et al, 1991), once they are easily available, easy to employ and can provide results rapidly (Jung and Maibach, 2015). Porcine ear skin and hairless mouse skin are widely employed, while shed snake skin has also potential to be used as a membrane model based on stratum corneum (SC) penetration rate (Praça et al, 2012;Baby et al, 2008;Nunes et al, 2005).…”
Section: Introductionmentioning
confidence: 99%
“…Different protocols are available to quantify the drug retained into porcine or hairless mouse skins, such as (i) evaluation of drug concentration in SC by the tape stripping technique (Lademann et al, 2009), (ii) evaluation of drug concentration in deeper skin layers by homogenization of skin tissue after tape stripping (viable epidermis without SC and dermis) (Campos et al, 2016;Depieri et al, 2015;Ascenso et al, 2014;Praça et al, 2011), (iii) evaluation of drug concentration in the whole skin tissue by homogenization (Ascenso et al, 2013), and (iv) assessment of skin drug penetration by specific micrometric sectioning of horizontal skin using a cryostat (Praça et al, 2012;Labouta et al, 2011;Echevarria et al, 2003). Among these methods, the homogenization technique of the whole skin tissue is easier, faster and less laborious, being also quite useful to detect a low drug penetration level.…”
Section: Introductionmentioning
confidence: 99%
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