Quantification of liver lipid deposition and melano-macrophages in lesser guitarfish Zapteryx brevirostris submitted to different feeding cycles

Neyrão, Iuri Moraes; Conrado, André Luiz Veiga; Takatsuka, Verônica; Bruno, Carlos Eduardo Malavasi; Azevedo, Venâncio Guedes de

doi:10.1007/s00580-019-02953-8

Cited by 3 publications

(1 citation statement)

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“…Likewise, in the present study, the down-regulation of bile acids biosynthesis, together with the decreased F/B ratio and the increased Spirochaetes phylum in the EWH group could be describing the link between the bioactive egg white hydrolysate and an anti-obesogenic response. This assumption is supported by the reduced lipid vacuolization in intestines, and by the restoration of normal liver fat deposition in association with an increase in the number of hepatic melanomacrophage centers, as already found during feed restriction in lesser guitarfish (Zapteryx brevirostris) (Neyrão et al, 2019). Studies addressing the gut metatranscriptome in close association to host changes of metabolism and intestinal transcriptome should be conducted to validate this hypothesis and unravel the molecular interactions behind the effects.…”

Section: Discussionmentioning

confidence: 62%

Modulation of Gilthead Sea Bream Gut Microbiota by a Bioactive Egg White Hydrolysate: Interactions Between Bacteria and Host Lipid Metabolism

et al. 2021

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This study aimed to highlight the relationship between diet, animal performance and mucosal adherent gut microbiota (anterior intestine) in fish fed plant-based diets supplemented with an egg white hydrolysate (EWH) with antioxidant and anti-obesogenic activity in obese rats. The feeding trial with juveniles of gilthead sea bream (Sparus aurata) lasted 8 weeks. Fish were fed near to visual satiety with a fish meal (FM)/fish oil (FO) based diet (CTRL) or a plant-based diet with/without EWH supplementation. Specific growth rate decreased gradually from 2.16% in CTRL fish to 1.88% in EWH fish due to a reduced feed intake, and a slight impairment of feed conversion ratio. Plant-based diets feeding triggered a hyperplasic inflammation of the anterior intestine regardless of EWH supplementation. However, EWH ameliorated the goblet cell depletion, and the hepatic and intestinal lipid accumulation induced by FM/FO replacement. Illumina sequencing of gut mucosal microbiota yielded a mean of 136,252 reads per sample assigned to 2,117 OTUs at 97% identity threshold. The bacterial diversity was similar in all groups, but a significantly lower richness was found in EWH fish. At the phylum level, Proteobacteria reached the highest proportion in CTRL and EWH fish, whereas Firmicutes were decreased and Actinobacteria increased with the FM/FO replacement. The proportion of Actinobacteria was restored by dietary EWH supplementation, which also triggered a highest amount of Bacteroidetes and Spirochaetes. At a closer look, a widespread presence of Lactobacillales among groups was found. Otherwise, polysaccharide hydrolases secretors represented by Corynebacterium and Nocardioides were increased by the FM/FO replacement, whereas the mucin-degrading Streptococcus was only raised in fish fed the plant-based diet without EWH. In addition, in EWH fish, a higher abundance of Propionibacterium was related to an increased concentration of intestinal propionate. The antagonism of gut health-promoting propionate with cholesterol could explain the inferred underrepresentation of primary bile acid biosynthesis and steroid degradation pathways in the EWH fish microbiota. Altogether, these results reinforce the central role of gut microbiota in the regulation of host metabolism and lipid metabolism in particular, suggesting a role of the bioactive EWH peptides as an anti-obesity and/or satiety factor in fish.

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Section: Discussionmentioning

confidence: 62%

Modulation of Gilthead Sea Bream Gut Microbiota by a Bioactive Egg White Hydrolysate: Interactions Between Bacteria and Host Lipid Metabolism

et al. 2021

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Eugenol and Lippia alba essential oils as effective anesthetics for the Amazonian freshwater stingray Potamotrygon wallacei (Chondrichthyes, Potamotrygonidae)

Lima

Morales-Gamba

Neto

et al. 2021

Fish Physiol Biochem

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This study assessed the potential of eugenol and the essential oil of Lippia alba (EOLA) in providing suitable anesthetic induction and recovery times, and their consequent effects on the blood and respiratory physiology, as well as the gill architecture of an Amazonian freshwater stingray, Potamotrygon wallacei, at the onset of the anesthetic event and after 48 h of recovery. Juveniles of P.wallacei (n = 12) were exposed to increasing concentrations of eugenol (75, 100, 125 and 150 µL L − 1 ) and EOLA (150, 175, 200 and 225 µL L − 1 ) in an immersion bath. Anesthetic induction was found to be faster with the use of eugenol compared to EOLA. On the other hand, the stingrays anesthetized with eugenol displayed a longer recovery time than those exposed to EOLA. The highest concentrations of eugenol caused moderate to severe histological changes in the gills. No signi cant changes were found for hematocrit and plasma metabolites in the stingrays anesthetized with all concentrations of both eugenol and EOLA just after the onset of anesthetic action, when compared to those recovered after 48 hours. Investigations regarding the potential use of these natural anesthetics are unprecedented for freshwater stingray species and 200 µL L − 1 EOLA is recommended as the most suitable anesthetic for use in juveniles of P. wallacei.

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Pathological study of a traumatic anthropogenic injury in the skeleton of a spiny butterfly ray (Gymnura altavela)

Montero-Hernández,

Caballero,

Curros-Moreno

et al. 2024

Front. Vet. Sci.

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IntroductionExternal injuries in elasmobranchs are frequent findings, either due to inter- or intraspecific interactions or as a result of interaction with human activities. However, the resilience of these species to traumatic injury remains poorly understood. This work provides an insight into the clinical presentation, diagnostic imaging, and pathological features of a severe traumatic injury to the cartilaginous skeleton of a spiny butterfly ray (Gymnura altavela).MethodsAn adult female was found lethargic in the bottom of the coast of Gran Canaria, with an external incised-contused traumatic lesion of 2 cm diameter in the scapulocoracoid cartilage. It was captured and transferred to the Poema del Mar Aquarium for its clinical evaluation and treatment. Despite these efforts, the animal eventually died and was transfer to the Institute of Animal Health and Food Safety (IUSA) for its pathological diagnosis, including a Computed Tomography (CT) study and necropsy.ResultsThe animal presented a marked reduction in hematocrit and hepatosomatic index due a chronic debilitation process. The CT scan revealed a destructive lesion with irregular margins at the level of the right scapulocoracoid cartilage. The main pathological findings were the disorganization of the tesserae layer, appearing as whitish square to rectangular geometric pieces separated from the cartilaginous core. Histologically, these pieces of tesserae were separated from the unmineralized cartilage core and displaced from the adjacent perichondrium, where inflammatory cells infiltrate. Edema and hemorrhages were also observed.ConclusionsThis study reports the first comprehensive description of skeleton trauma in a spiny butterfly ray, including the clinical presentation, diagnostic imaging and the anatomopathological features.

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Quantification of liver lipid deposition and melano-macrophages in lesser guitarfish Zapteryx brevirostris submitted to different feeding cycles

Cited by 3 publications

References 25 publications

Modulation of Gilthead Sea Bream Gut Microbiota by a Bioactive Egg White Hydrolysate: Interactions Between Bacteria and Host Lipid Metabolism

Modulation of Gilthead Sea Bream Gut Microbiota by a Bioactive Egg White Hydrolysate: Interactions Between Bacteria and Host Lipid Metabolism

Eugenol and Lippia alba essential oils as effective anesthetics for the Amazonian freshwater stingray Potamotrygon wallacei (Chondrichthyes, Potamotrygonidae)

Pathological study of a traumatic anthropogenic injury in the skeleton of a spiny butterfly ray (Gymnura altavela)

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