Quantification of major bioactive withanolides in<i>Withania somnifera</i>(Ashwagandha) roots by HPTLC for rapid validation of Ayurvedic products

Journal of Planar Chromatography – Modern TLC

Jagtap

Katyare³

et al. 2014

Self Cite

Section: Resultsmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Estimation of antioxidant potential of individual components present in complex mixture ofWithania somnifera(Ashwagandha) root fraction by thin-layer chromatography-2,2-diphenyl-1-picrylhdrazyl method

Journal of Planar Chromatography – Modern TLC

Jagtap

Katyare³

et al. 2014

Self Cite

“…These medicinal properties are mainly attributed to withanolides present in the roots of W. somnifera (Kulkarni et al 2008). Withanolides are steroidal lactones and about 35 withanolides have been isolated from the roots of W. somnifera and quantified (Mishra et al 2000;Kulkarni et al 2008;Devkar et al 2012Devkar et al , 2013. The multi-component extracts show better medicinal efficacy than purified components, i.e., the strength of herbal medicine increased with the mixture of secondary metabolites instead of a single component (Patwardhan & Mashelkar 2009).…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective effect of withanolide-rich fraction in acetaminophen-intoxicated rat: decisive role of TNF-α, IL-1β, COX-II and iNOS

Kandhare

Zanwar

et al. 2016

Pharmaceutical Biology

“…The chromatogram was run up to 80 mm. Quantitative evaluation of the plate was performed in the absorption reflection mode at 235 nm, tungsten (W) lamp and deuterium (D2), using slit width: 4.00 Â 0.30 mm, data resolution 100 mm/step, scanning speed: 20 mm/s, and base line correction was used (Devkar et al, 2012(Devkar et al, , 2014.…”

Section: Quantification Of Major Bioactive Withanolides (Mw)mentioning

confidence: 99%

Effect of macronutrient deficiency on withanolides content in the roots ofWithania somniferaand its correlationship with molybdenum content

Suryapujary

Jagtap

et al. 2014

Pharmaceutical Biology

Context: The content of withanolides in the roots of Withania somnifera (L.) Dunal (Solanaceae) is important for therapeutic application. Earlier studies have shown that the deficiency of macro-and micronutrients affects the growth of W. somnifera. Therefore, we examined the effect of these deficiencies on the withanolides content of the roots. Objective: To examine the effect of molybdenum accretion in nitrogen-, phosphorus-, calciumand potassium-deficient soils on the accumulation of withanolides in the roots of W. somnifera. Different withanolides have different therapeutic applications hence major bioactive withanolides assume importance. Materials and methods: Methanol extracts of the roots were subjected to HPTLC and individual withanolides were identified by comparing their R f values with those of the authentic samples. Molybdenum was quantified by atomic absorption spectroscopy. Free radical scavenging activity was monitored by the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay. Results: Molybdenum content in roots of nitrogen-, phosphorus-, calcium-, potassium-deficient, and control plants were 7.02 ± 2.1, 13.1 ± 1.6, 17.1 ± 0.9, 33.5 ± 3.3, and 33.9 ± 1.6 ppm, respectively. Levels of withaferine A increased with the increase in the Mo content in roots from 7.79 ± 2.2 mg/g to 12.57 ± 3.4 mg/g. Antioxidant activity of nitrogen-deficient plants was the lowest (24.7 ± 2.2%) compared to other groups. Discussion and conclusion: It was observed that nitrogen metabolism-dependent molybdenum uptake influences the withanolides accumulation in the roots.