Quantification of membrane fluidity in bacteria using TIR-FCS

Barbotin, Aurélien; Billaudeau, Cyrille; Sezgin, Erdinc; Carballido-López, Rut

doi:10.1016/j.bpj.2024.06.012

Biophysical Journal

2024

DOI: 10.1016/j.bpj.2024.06.012

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Quantification of membrane fluidity in bacteria using TIR-FCS

Aurélien Barbotin,

Cyrille Billaudeau,

Erdinc Sezgin

et al.

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2024

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Aquatic environment drives the emergence of cell wall-deficient dormant forms in Listeria

Carvalho,

Carreaux,

Sartori-Rupp

et al. 2024

Nat Commun

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Stressed bacteria can enter a dormant viable but non-culturable (VBNC) state. VBNC pathogens pose an increased health risk as they are undetectable by growth-based techniques and can wake up back into a virulent state. Although widespread in bacteria, the mechanisms governing this phenotypic switch remain elusive. Here, we investigate the VBNC state transition in the human pathogen Listeria monocytogenes . We show that bacteria starved in mineral water become VBNC by converting into osmotically stable cell wall-deficient coccoid forms, a phenomenon that occurs in other Listeria species. We reveal the bacterial stress response regulator SigB and the autolysin NamA as major actors of VBNC state transition. We lastly show that VBNC Listeria revert to a walled and virulent state after passage in chicken embryos. Our study provides more detail on the VBNC state transition mechanisms, revealing wall-free bacteria naturally arising in aquatic environments as a potential survival strategy in hypoosmotic and oligotrophic conditions.

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Aquatic environment drives the emergence of cell wall-deficient dormant forms in Listeria

Carvalho,

Carreaux,

Sartori-Rupp

et al. 2024

Nat Commun

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Quantification of membrane fluidity in bacteria using TIR-FCS

Cited by 1 publication

References 75 publications

Aquatic environment drives the emergence of cell wall-deficient dormant forms in Listeria

Aquatic environment drives the emergence of cell wall-deficient dormant forms in Listeria

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