Quantification of metabolically active biomass using Methylene Blue dye Reduction Test (MBRT): Measurement of CFU in about 200 s

Bapat, Prashant M.; Nandy, Subir Kumar; Wangikar, Pramod P.; Venkatesh, K. V.

doi:10.1016/j.mimet.2005.06.010

Cited by 75 publications

(64 citation statements)

References 22 publications

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“…Milk as it exists in the udder, or milk drawn anaerobically, reduces methylene blue almost instantaneously, whereas the same milk exposed to oxygen will usually take more than ten hours to reduce. The oxidation-reduction potential of anaerobically drawn milk is much lower than the same milk exposed to oxygen, and in accordance with its atalyze toward methylene blue (Bapat et al, 2006). Evidence is given for the presence of a redox system, present in low concentration, as responsible for the reduction of methylene blue.…”

Section: Indigenous and Exotic Breeds Of Cattlementioning

confidence: 64%

“…For this purpose, the slope of the MB decolouration rate was calibrated with respect to colony forming units (CFU) obtained through plating. This method was successfully employed to characterize the viability of E. coli and B. subtilis (Bapat et al, 2006). Studies revealed that MBRT can be successfully employed to quantify viable cell count in a very short time (less than 4min).…”

Section: Indigenous and Exotic Breeds Of Cattlementioning

confidence: 99%

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Study of some Indigenous and Exotic Cattle Breeds and a Comparative Analysis of their Milk Quality using MBRT

Mini¹

2018

IJRASET

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The principle of methylene blue reduction test depends on the fact that the color imparted to the milk by adding a dye such as methylene blue will disappear more or less quickly, which depends on the quality of the milk sample to be examined. Methylene blue is a redox indicator, that lose its color under the absence of oxygen and is thought to be reduced. The depletion of oxygen in the milk is due to the production of reducing substances in the milk due to the enhanced rate of bacterial metabolism. The dye reduction time refers to the microbial load in the milk and the total metabolic reactions of the microorganism.

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Section: Indigenous and Exotic Breeds Of Cattlementioning

confidence: 64%

Section: Indigenous and Exotic Breeds Of Cattlementioning

confidence: 99%

Study of some Indigenous and Exotic Cattle Breeds and a Comparative Analysis of their Milk Quality using MBRT

Mini¹

2018

IJRASET

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“…MBR test is an indirect method of estimating bacterial population (Bapat et al, 2006;Nandy and Venkatesh, 2010). Methylene blue is a redox indicator that loses its color under the absence of oxygen.…”

Section: Methylene Blue Reduction (Mbr) Testmentioning

confidence: 99%

Use of Hydrogen Peroxide (H2O2) in raw cow's milk preservation

Arefin¹,

Sarker²,

Islam³

et al. 2017

J Adv Vet Anim Res

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Objective: Hydrogen peroxide (H2O2) was used for the activation of lactoperoxidase system on preservation of milk. Materials and methods: Milk samples were collected from Bangladesh Agricultural University dairy farm. The collected milk samples were added with 0.02, 0.04, 0.06, 0.08, 0.10, 0.12 and 0.14% of 10% H2O2 along with control one. All milk samples were kept at room temperature (28-31 0 C). Flavor, clot on boiling (COB), acidity %, and methylene blue reduction (MBR) test were observed every one hour interval. Results: The milk samples were acceptable in terms of flavor up to 18 h at 0.14% H2O2. Similarly, this milk sample took maximum time (19 h) to give COB positive test. Acidity % was within normal range for 0.02, 0.04, 0.06, 0.08, 0.10, 0.12 and 0.14% H2O2 treated milk samples up to 8,9,11,12,12,14,17 and 18 h, respectively. Significant difference (P<0.01) was found among the levels of H2O2 in acidity development at every observation hour except 0 hour. In MBR test, control sample was decolorized at 7 h and H2O2 treated milk samples were decolorized after 8 to 13 h depending on the level of added H2O2. Conclusion: Based on the results, it may be recommended that 0.14% H2O2 is suitable to extend the milk shelf-life where milk cooling facilities are not available.

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“…Besides viable and physiologically active biomass (Bapat et al 2005), fermentation media is an important variable, which decides the fate of the fermentation. The fermentation media components can affect the fermentation process at all stages including seed maturation (Bapat et al 2003) and production (Bapat and Wangikar 2004).…”

Section: Introductionmentioning

confidence: 99%

A cybernetic model to predict the effect of freely available nitrogen substrate on rifamycin B production in complex media

Bapat

Sohoni

Moses

et al. 2006

Appl Microbiol Biotechnol

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A cybernetic model to predict the effect of freely available nitrogen substrate on rifamycin B production in complex media Abstract It is well-known that secondary metabolite production is repressed by excess nitrogen substrate available in the fermentation media. Although the nitrogen catabolite repression has been known, quantitative process models have not been reported to represent this phenomenon in complex medium. In this paper, we present a cybernetic model for rifamycin B production via Amycolatopsis mediterranei S699 in complex medium, which is typically used in industry. Nitrogen substrate is assumed to be present in two forms in the medium; available nitrogen (S ANS ) such as free amino acids and unavailable nitrogen (S UNS ) such as peptides and proteins. The model assumes that an inducible enzyme catalyzes the conversion of S UNS to S ANS . Although S ANS is required for growth and product formation, high concentrations were found to inhibit rifamycin production. To experimentally validate the model, five different organic nitrogen sources were used that differ in the ratio of S ANS / S UNS . The model successfully predicts higher rifamycin B productivity for nitrogen sources that contain lower initial S ANS . The higher productivity is attributed to the sustained availability of S ANS at low concentration via conversion of S UNS to S ANS , thereby minimizing the effects of nitrogen catabolite repression on rifamycin production. The model can have applications in model-based optimization of substrate feeding recipe and in monitoring and control of fed batch processes.

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Quantification of metabolically active biomass using Methylene Blue dye Reduction Test (MBRT): Measurement of CFU in about 200 s

Cited by 75 publications

References 22 publications

Study of some Indigenous and Exotic Cattle Breeds and a Comparative Analysis of their Milk Quality using MBRT

Study of some Indigenous and Exotic Cattle Breeds and a Comparative Analysis of their Milk Quality using MBRT

Use of Hydrogen Peroxide (H2O2) in raw cow's milk preservation

A cybernetic model to predict the effect of freely available nitrogen substrate on rifamycin B production in complex media

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