Quantification of monoterpene emission sources of a conifer species in response to experimental drought

Lüpke, Marvin; Leuchner, Michael; Steinbrecher, R.; Menzel, Annette

doi:10.1093/aobpla/plx045

Cited by 17 publications

(17 citation statements)

References 69 publications

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“…1). Similar reductions in gas exchange and Ψ plant were seen in water‐stressed Scots pine seedlings (Lüpke et al ., 2016, 2017) and other conifers (Tan & Blake, 1997; Kleiber et al ., 2017). Noteworthy, Ψ plant of the stressed trees ranged from −1.5 to −2.0 MPa and, therefore, was still higher than the −2.5 MPa demonstrated to cause air embolism in the xylem of Scots pine (Cochard, 1992).…”

Section: Discussionmentioning

confidence: 99%

“…By contrast, isoprene, monoterpenes and diterpenes are mainly synthesised via the plastidic methyl erythritol phosphate (MEP) pathway (Kreuzwieser et al ., 1999; Vranová et al ., 2012; Dudareva et al ., 2013) using pyruvate and glyceraldehyde‐3‐phosphate (G3P) as substrate. The main portion of these precursors originates from the Benson–Calvin cycle in chloroplasts, therefore directly linking photosynthetic CO 2 fixation with terpene biosynthesis (Ghirardo et al ., 2010, 2014; Lüpke et al ., 2017). This has been demonstrated using 13 CO 2 fumigation, which caused a very fast incorporation of the 13 C into isoprene or monoterpenes (Delwiche & Sharkey, 1993; Karl et al ., 2002; Schnitzler at al., 2004; de Souza et al ., 2018).…”

Section: Introductionmentioning

confidence: 99%

“…The 13 C‐tracer experiments were performed with seedlings of Pinus sylvestris , a conifer with particular importance for European forestry. As a typical conifer, this species mainly emits monoterpenes (Janson, 1993; Komenda & Koppmann, 2002; Tarvainen et al ., 2005; Holzke et al ., 2006; Raisanen et al ., 2009; Bäck et al ., 2012; Kaennaste et al ., 2013; Aydin et al ., 2014; Luepke et al ., 2016, 2017) and, to a lower extent, isoprene, sesquiterpenes and oxygenated VOCs (Bäck et al ., 2005, 2012; Ruuskanen et al ., 2005; Holzke et al ., 2006; Lüpke et al ., 2016).…”

Section: Introductionmentioning

confidence: 99%

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Drought affects carbon partitioning into volatile organic compound biosynthesis in Scots pine needles

et al. 2021

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Summary The effect of drought on the interplay of processes controlling carbon partitioning into plant primary and secondary metabolisms, such as respiratory CO2 release and volatile organic compound (VOC) biosynthesis, is not fully understood. To elucidate the effect of drought on the fate of cellular C sources into VOCs vs CO2, we conducted tracer experiments with 13CO2 and position‐specific 13C‐labelled pyruvate, a key metabolite between primary and secondary metabolisms, in Scots pine seedlings. We determined the stable carbon isotope composition of leaf exchanged CO2 and VOC. Drought reduced the emission of the sesquiterpenes α‐farnesene and β‐farnesene but did not affect 13C‐incorporation from 13C‐pyruvate. The labelling patterns suggest that farnesene biosynthesis partially depends on isopentenyl diphosphate crosstalk between chloroplasts and cytosol, and that drought inhibits this process. Contrary to sesquiterpenes, drought did not affect emission of isoprene, monoterpenes and some oxygenated compounds. During the day, pyruvate was used in the TCA cycle to a minor degree but was mainly consumed in pathways of secondary metabolism. Drought partly inhibited such pathways, while allocation into the TCA cycle increased. Drought caused a re‐direction of pyruvate consuming pathways, which contributed to maintenance of isoprene and monoterpene production despite strongly inhibited photosynthesis. This underlines the importance of these volatiles for stress tolerance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Drought affects carbon partitioning into volatile organic compound biosynthesis in Scots pine needles

et al. 2021

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“…Additionally, upon wounding, constitutive terpenes are released to form a physical barrier to protect the stem from further infection (Trapp & Croteau, 2001). Other volatile profiling studies in forest tree species include: (i) natural terpene variation in VOC emission among populations (Welter et al, 2012); (ii) VOC profiling in response to environmental stresses, namely drought (Blanch et al, 2009; Eller et al, 2016; Kleiber et al, 2017; Lüpke et al, 2017), and (iii) EO chemotype occurrence (Keszei, Brubaker, & Foley, 2008), and EO responses of Pinus spp to B. xylophilus infection (Rodrigues et al, 2017, further explored in Section III.B).…”

Section: Ms‐based Analytical Platforms In Forest Tree Metabolomicsmentioning

confidence: 99%

Mass spectrometry‐based forest tree metabolomics

Rodrigues

Miguel

Chaves

et al. 2019

Mass Spectrometry Reviews

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Research in forest tree species has advanced slowly when compared with other agricultural crops and model organisms, mainly due to the long‐life cycles, large genome sizes, and lack of genomic tools. Additionally, trees are complex matrices, and the presence of interferents (e.g., oleoresins and cellulose) challenges the analysis of tree tissues with mass spectrometry (MS)‐based analytical platforms. In this review, advances in MS‐based forest tree metabolomics are discussed. Given their economic and ecological significance, particular focus is given to Pinus, Quercus, and Eucalyptus forest tree species to better understand their metabolite responses to abiotic and biotic stresses in the current climate change scenario. Furthermore, MS‐based metabolomics technologies produce large and complex datasets that require expertize to adequately manage, process, analyze, and store the data in dedicated repositories. To ensure that the full potential of forest tree metabolomics data are translated into new knowledge, these data should comply with the FAIR principles (i.e., Findable, Accessible, Interoperable, and Re‐usable). It is essential that adequate standards are implemented to annotate metadata from forest tree metabolomics studies as is already required by many science and governmental agencies and some major scientific publishers. © 2019 John Wiley & Sons Ltd. Mass Spec Rev 40:126–157, 2021.

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“…Droughts are expected to become more frequent throughout the 21 st century 16 , causing disruptions to the functioning of ecosystems 17 and emissions of volatile organic compound from forests 18 . Reported monoterpene emission responses to drought are highly variable and dependent on the individual plant, making empirically based achiral emission inventories intractable [19][20][21][22][23] . However, since chiral compounds link directly to the underlying enzymatically driven processes they could form the basis of an improved emission scheme.…”

Section: Introductionmentioning

confidence: 99%

Chiral monoterpenes reveal forest emission mechanisms and drought responses

Byron¹,

Kreuzwieser²,

Purser³

et al. 2021

Preprint

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Monoterpenes exist in mirror image forms called enantiomers, but their individual formation pathways in plants and ecological functions are poorly understood, as enantiomers are usually measured and modelled together. Here we present enantiomerically separated atmospheric monoterpene and isoprene data from an enclosed tropical rainforest ecosystem without photo-chemistry during a four-month controlled drought and rewetting experiment. Surprisingly, the enantiomers showed distinct diel emission peaks, which responded differently to progressive drying. Isotopic labelling established that vegetation emitted (-)-α-pinene mainly de novo while (+)-α-pinene was emitted from storage pools. As drought stress increased, (-)-α-pinene emis-sions shifted to storage pools, which are released later in the day, favouring cloud formation. The α-pinene enantiomers each correlated better with other monoterpenes than with each other, indicating different enzymatic controls. These results show that enantiomeric distribution is key to understanding the processes driving monoterpene emission from ecosystems and to predict-ing atmospheric feedbacks in response to climate change.

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Quantification of monoterpene emission sources of a conifer species in response to experimental drought

Cited by 17 publications

References 69 publications

Drought affects carbon partitioning into volatile organic compound biosynthesis in Scots pine needles

Drought affects carbon partitioning into volatile organic compound biosynthesis in Scots pine needles

Mass spectrometry‐based forest tree metabolomics

Chiral monoterpenes reveal forest emission mechanisms and drought responses

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