Quantification of N-acetylcysteine in pharmaceuticals using cobalt phthalocyanine modified graphite electrodes

Silva, Iranaldo Santos da; Araújo, Marcelo Fernando Antão; Ferreira, Henrique Augusto; Júnior, Jaldyr de Jesus Gomes Varela; Tanaka, Sônia Maria Carvalho Neiva; Tanaka, Auro Atsushi; Angnes, Lúcio

doi:10.1016/j.talanta.2010.11.070

Cited by 32 publications

(8 citation statements)

References 25 publications

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“…The results obtained from bare SPE were also given in Figure 1 for comparison. In sulfur containing a NaOH solution, the cyclic voltammetric response of bare SPE was featureless (Figure 1a), while in the presence of a CoPc modified SPE, a reproducible and well defined faradic response to sulfur was obtained at E pa − 0.30 V and E pc − 0.27 V ( vs. Ag/AgCl) (Figure 1b) in the potential range of − 0.2 V to 0.6 V. During the transfer of Co I to Co II and Co II to Co I for the cathodic and anodic peak, similar electrochemcal behavior was reported [26]. This observation clearly demonstrates an electro-catalytic mediation of the CoPc towards the sulfur oxidation reaction.…”

Section: Resultssupporting

confidence: 76%

Screen-Printed Carbon Electrodes Modified with Cobalt Phthalocyanine for Selective Sulfur Detection in Cosmetic Products

Chen

Luo

Chen

et al. 2011

IJMS

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Cobalt phthalocyanine (CoPc) films were deposited on the surface of a screen-printed carbon electrode using a simple drop coating method. The cyclic voltammogram of the resulting CoPc modified screen-printed electrode (CoPc/SPE) prepared under optimum conditions shows a well-behaved redox couple due to the (CoI/CoII) system. The CoPc/SPE surface demonstrates excellent electrochemical activity towards the oxidation of sulfur in a 0.01 mol·L−1 NaOH. A linear calibration curve with the detection limit (DL, S/N = 3) of 0.325 mg·L−1 was achieved by CoPc/SPE coupled with flow injection analysis of the sulfur concentration ranging from 4 to 1120 mg·L−1. The precision of the system response was evaluated (3.60% and 3.52% RSD for 12 repeated injections), in the range of 64 and 480 mg·L−1 sulfur. The applicability of the method was successfully demonstrated in a real sample analysis of sulfur in anti-acne creams, and good recovery was obtained. The CoPc/SPE displayed several advantages in sulfur determination including easy fabrication, high stability, and low cost.

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Section: Resultssupporting

confidence: 76%

Screen-Printed Carbon Electrodes Modified with Cobalt Phthalocyanine for Selective Sulfur Detection in Cosmetic Products

Chen

Luo

Chen

et al. 2011

IJMS

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“…Flow injection analysis (FIA) with amperometric detection was employed for the quantification of N-acetylcysteine (NAC) in pharmaceutical formulations, utilizing an ordinary pyrolytic graphite (OPG) electrode modified with cobalt phthalocyanine (CoPc) [121]. Cyclic voltammetry was used in preliminary studies to establish the best conditions for NAC analysis.…”

Section: Modified Graphite Electrodesmentioning

confidence: 99%

“…For the flow injection analysis experiments, a simple flow cell was built in house (Fig. 6A) [121] in such a way as to fit the same pyrolytic carbon electrode previously employed in the voltammetric experiments. A miniaturized Ag/AgCl/KCl sat electrode was constructed in the laboratory for use in the same flow cell [122].…”

Section: Modified Graphite Electrodesmentioning

confidence: 99%

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Application of phthalocyanines in flow- and sequential-injection analysis and microfluidics systems: A review

Staden

2015

Talanta

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“…Recent studies have revealed that NAC inhibits muscle fatigue and can be used to enhance performance in exercise and endurance training. [1][2][3] Analytical techniques like colorimetry, 4,5 chemiluminiscence, 6,7 electrochemical detection, [8][9][10][11][12][13][14][15] flurimetry, 16,17 turbidimetry and nephlometry, 18 liquid chromatography tandem mass spectrometry, 19-21-44 gas chromatography mass spectrometry, 22,23 and capillary electrophoresis [24][25][26] have been employed in literature for the quantification of acetyl cysteine. Acetyl cysteine has also been simultaneously quantified along with other drugs like clomiphene citrate, 27 arginine, 28 and cefexime trihydrate.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an Analytical Method for Related Substances in N-acetyl–L-cysteine Effervescent Tablets by RP-HPLC

Mathew¹,

Ravi²,

Rameshwar³

et al. 2017

IJPER

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Background:The reported chromatographic methods for N-acetyl cysteine [NAC] are reverse phase HPLC and ion pair chromatography [IPC] for related substances test in bulk and in formulations. No reported stability indicating methods for the estimation of related substances in NAC effervescent formulation was found in literature. Objective: The present work was aimed at developing a selective, sensitive and reproducible stability indicating high-performance liquid chromatographic method for the quantitative determination of known, unknown impurities, degradation impurities and processrelated impurities of NAC effervescent formulation. Method: A reversed phase ion pair chromatographic method was developed employing Cadenza C18 column as the stationary phase and 0.01M octane sulphonate [pH 2.20], methanol and acetonitrile in the ratio 90:8:2 as the mobile phase. A gradient programme was followed with a run time of 55 minutes. 0.3 M hydrochloric acid was selected as the optimum diluent. The performance of the method was validated according to the ICHQ2R1 guidelines. Results: The method was found to be linear from 1.5 to 25µg/ml for impurities A, C and D and from 2.0 to 25 µg/ml for impurity B. The official impurities C and D were mapped in all stress conditions. Additionally, impurity B was also seen in acidic conditions. Conclusion: The results from the study demonstrate that the method is suitable for evaluating the stability of NAC effervescent tablet. Key words: N-acetyl cysteine, Reverse phase HPLC, Effervescent formulation, Ion pair chromatography, Related substances,Validation. Key message: An ion pair chromatographic method was developed for quantifying the related substances of N-acetyl cysteine effervescent Tablets. Selection of diluent was an important variable in the method development.

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Quantification of N-acetylcysteine in pharmaceuticals using cobalt phthalocyanine modified graphite electrodes

Cited by 32 publications

References 25 publications

Screen-Printed Carbon Electrodes Modified with Cobalt Phthalocyanine for Selective Sulfur Detection in Cosmetic Products

Screen-Printed Carbon Electrodes Modified with Cobalt Phthalocyanine for Selective Sulfur Detection in Cosmetic Products

Application of phthalocyanines in flow- and sequential-injection analysis and microfluidics systems: A review

Development and Validation of an Analytical Method for Related Substances in N-acetyl–L-cysteine Effervescent Tablets by RP-HPLC

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