Quantification of Nineteen Bioactive Components in the Ancient Classical Chinese Medicine Formula of Wen-Dan Decoction and Its Commercial Preparations by UHPLC-QQQ-MS/MS

Abstract

A UHPLC-QQQ-MS/MS method was developed to quantify the significant constituents in Wen-Dan Decoction (WDD), a traditional Chinese medicine. Analysis of 19 compounds was conducted on an ACQUITY UPLC® BEH C18 Column (2.1 × 50 mm, 1.7 μm) using elution with a gradient elution of acetonitrile and 0.05% (v/v) formic acid in water. A triple quadrupole mass spectrometer was operated in negative ionization mode and positive ionization mode by multiple reaction monitoring (MRM), respectively. All calibration curves showed acceptable linearity (r ≥ 0.9950). The RSDs of intra- and inter-day precisions of low, mid and high concentrations were ≤ 8.88%. The repeatabilities (RSDs ≤ 7.17%) and stabilities (RSD ≤ 4.79%) of the samples were qualified. The recoveries were found in the range of 93.07 ± 3.86 to 103.98 ± 2.98% with the RSD varying between 1.30 and 7.86%. The final rapid, sensitive, precise, accurate and reliable UHPLC-QQQ-MS/MS method was used for the simultaneous quantification of 19 constituents in WDD and its commercial preparations. The strategy of combining the contents of the 19 chemicals in a daily dose of the WDD preparations with the hierarchical cluster analysis and the 3D principal component analysis was employed to effectively distinguish the WDD preparations provided by the different suppliers, which represents a contribution to the evaluation and control of the quality of WDD (or other decoctions consisting of the same herbs) and the preparations of WDD in other dosage forms such as tablets and granules.

Wen Dan Tang: A Potential Jing Fang Decoction for Headache Disorders?

“…Modern scientific research provided evidence that WDT contains compounds including flavonoids, phenols, alkaloids, triterpenoid organic acid, polysaccharides, and phosphodiesterase inhibitors ( Table 3 ), which have been found to possess neuroprotective, neuromodulation, anti-mutagenic, antioxidant, antiemetic, antithrombotic, antipyretic, and anti-inflammatory effects [ 2 , 30 , 46 , 47 , 48 , 49 , 50 , 51 , 52 ].…”

“…Wen Dan Tang (WDT), also known as “Warm the Gallbladder decoction”, is a classical famous Chinese herbal formula containing eight components derived from the book Treatise on Three Categories of Pathogenic Factors: Pinellia ternata (Thunb.) Makino (Ban Xia), Bambusa tuldoides Munro (Zhu Ru), Citrus aurantium L. (Zhi Shi), Citrus reticulata Blanco (Chen Pi), Glycyrrhiza uralensis Fisch (Gan Cao), Poriae sclerotium cocos albae (Fu Ling), Zingiber officinale Roscoe (Sheng Jiang), and Ziziphus jujuba Mil (Da Zao) ( Table 1 ) [ 1 , 2 ].…”

“…The complete extraction method could be performed within 30 min in the lab. This procedure is thus not more time-consuming than the traditional method, and more pure gingerol extracts can be obtained with it [ 2 ].…”

Versatile Medicine? A Mini Review on the Therapeutic Approach of Chinese Medicine for Pediatric Obesity

“…Some of them are nonintrusive. A VBM-DARTEL study revealed that microstructural difference might exit in brain because if obesity, and acupuncture therapy might influence the process of brain development, leading to reorganization of cortices on the obese children [13]. Herbal preparation is multifarious.…”

Study on the Fingerprints and Quality Evaluation of Angelica Sinensis Radix by HPLC Coupled With Chemometrics Based on Traditional Decoction Process of ACPTCM

“…Several research on classical prescriptions have been developed. 6 - 15 For instance, Danggui Buxue Tang (DBT), an ancient formula composed of Angelica Sinensis Radix (ASR) and Astragali Radix (AR), first recorded in “Neiwaishang Bianhuo Lun” 16 by Li, Dongyuan in China in AD 1247. In TCM clinics, DBT is prescribed for women to improve their health by raising the “Qi” (vital energy) and nourishing the “Blood” (body circulation), 17 , 18 and thus DBT today is a remedy for menopausal women to promote hematopoiesis, stimulate cardiovascular circulation, prevent osteoporosis, and trigger estrogenic activity.…”