Quantification of ondansetron, granisetron and tropisetron in goat plasma using hydrophilic interaction liquid chromatography-solid phase extraction coupled with hydrophilic interaction liquid chromatography-triple quadrupole tandem mass spectrometry

A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT3 Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker

“…According to the literature [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24], acetonitrile and acidic water media were selected to optimize the mobile phase composition. A series of concentrations, pH values, the ratio of aqueous potassium dihydrogen phosphate solution, and different chromatographic column types were studied to ensure good resolution and appropriate retention time of the five 5-HT 3 receptor antagonists.…”

“…HPLC is commonly used to verify rapid detection systems due to its attractive features, such as high peak efficiencies, great resolution, high sensitivity, good repeatability, and wide application range. HPLC analytic methods for the detection of 5-HT 3 receptor antagonists such as ODT, GNT, TPT, AZT, and RMT are already mature, and most of them use retention data in the qualitative analysis, while the external standard method (ESM) is applicable in quantitative analysis [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24]. However, some of these methods may take a long time, and the specific drugs to be tested must correspond to reference substances, which cannot meet the requirements of rapid drug detection.…”