Quantification of peripheral whole blood, cell-free plasma and exosome encapsulated mitochondrial DNA copy numbers in patients with atrial fibrillation

Soltész, Beáta; Urbancsek, Réka; Pös, Ondrej; Hajas, Orsolya; Forgács, Ildikó Noémi; Szilágyi, Edina; Nagy‐Baló, Edina; Szemes, Tomáš; Csanádi, Zoltán; Nagy, Bálint

doi:10.1016/j.jbiotec.2019.04.018

Cited by 24 publications

(18 citation statements)

References 35 publications

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“…While other studies report exosomes contain mtDNA [8,[33][34][35], these studies did not account for potential confounding by NUMT DNA sequences. The presence of NUMT DNA could give the false impression that mtDNA is present within exosomes when it is not, or skew quantification of truly internalized authentic mtDNA.…”

Section: Discussionmentioning

confidence: 94%

Detection of mitochondria-pertinent components in exosomes

et al. 2020

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Section: Discussionmentioning

confidence: 94%

Detection of mitochondria-pertinent components in exosomes

et al. 2020

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“…It was also demonstrated that both normal cells and cultured tumor cells are able to secrete their intact, respiratory competent mitochondria [50]. Several studies have linked cf-mtDNA copy number changes to various human diseases, including cancer, neurodegenerative or cardiovascular diseases [51]. Reduced cf-mtDNA levels in the cerebrospinal fluid of neurodegenerative disease patients-including Alzheimer's and Parkinson's disease as well as multiple sclerosis-have also been reported.…”

Section: Cell-free Mtdnamentioning

confidence: 99%

Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application

Szilágyi

Pös

Márton

et al. 2020

IJMS

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150

108

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Liquid biopsy recently became a very promising diagnostic method that has several advantages over conventional invasive methods. Liquid biopsy may serve as a source of several important biomarkers including cell-free nucleic acids (cf-NAs). Cf-DNA is widely used in prenatal testing in order to characterize fetal genetic disorders. Analysis of cf-DNA may provide information about the mutation profile of tumor cells, while cell-free non-coding RNAs are promising biomarker candidates in the diagnosis and prognosis of cancer. Many of these markers have the potential to help clinicians in therapy selection and in the follow-up of patients. Thus, cf-NA-based diagnostics represent a new path in personalized medicine. Although several reviews are available in the field, most of them focus on a limited number of cf-NA types. In this review, we give an overview about all known cf-NAs including cf-DNA, cf-mtDNA and cell-free non-coding RNA (miRNA, lncRNA, circRNA, piRNA, YRNA, and vtRNA) by discussing their biogenesis, biological function and potential as biomarker candidates in liquid biopsy. We also outline possible future directions in the field.

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“…Interestingly, mtDNAs can be encapsulated into extracellular vesicles (EVs) [65,66]. EVs are able to transfer mtDNAs between cells.…”

Section: Mtdnas Mediate Cell-to-cell Communicationsmentioning

confidence: 99%

Extrachromosomal Circular DNAs: Origin, formation and emerging function in Cancer

Wang¹,

Chen²,

Yu³

et al. 2021

Int. J. Biol. Sci.

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The majority of cellular DNAs in eukaryotes are organized into linear chromosomes. In addition to chromosome DNAs, genes also reside on extrachromosomal elements. The extrachromosomal DNAs are commonly found to be circular, and they are referred to as extrachromosomal circular DNAs (eccDNAs). Recent technological advances have enriched our knowledge of eccDNA biology. There is currently increasing concern about the connection between eccDNA and cancer. Gene amplification on eccDNAs is prevalent in cancer. Moreover, eccDNAs commonly harbor oncogenes or drug resistance genes, hence providing a growth or survival advantage to cancer cells. eccDNAs play an important role in tumor heterogeneity and evolution, facilitating tumor adaptation to challenging circumstances. In addition, eccDNAs have recently been identified as cell-free DNAs in circulating system. The altered level of eccDNAs is observed in cancer patients relative to healthy controls. Particularly, eccDNAs are associated with cancer progression and poor outcomes. Thus, eccDNAs could be useful as novel biomarkers for the diagnosis and prognosis of cancer. In this review, we summarize current knowledge regarding the formation, characteristics and biological importance of eccDNAs, with a focus on the molecular mechanisms associated with their roles in cancer progression. We also discuss their potential applications in the detection and treatment of cancer. A better understanding of the functional role of eccDNAs in cancer would facilitate the comprehensive analysis of molecular mechanisms involved in cancer pathogenesis.

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Quantification of peripheral whole blood, cell-free plasma and exosome encapsulated mitochondrial DNA copy numbers in patients with atrial fibrillation

Cited by 24 publications

References 35 publications

Detection of mitochondria-pertinent components in exosomes

Detection of mitochondria-pertinent components in exosomes

Circulating Cell-Free Nucleic Acids: Main Characteristics and Clinical Application

Extrachromosomal Circular DNAs: Origin, formation and emerging function in Cancer

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