Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection

Møller, Laura Hyrup; Macherius, André; Hansen, Thomas H.; Nielsen, Hanne Mørck; Cornett, Claus; Østergaard, Jesper; Stürup, Stefan; Gammelgaard, Bente

doi:10.1039/c6ja00132g

Cited by 15 publications

(12 citation statements)

References 41 publications

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“…Inductively coupled plasma mass spectrometry (ICP‐MS) is a system for measuring metal elements, and is in fact the most sensitive method for elemental analysis . A laser ablation inductively coupled plasma imaging mass spectrometry (LAICP‐IMS) method has been developed for highly sensitive and quantitative surface analysis of metal elements in solid samples .…”

Section: Introductionmentioning

confidence: 99%

“…Inductively coupled plasma mass spectrometry (ICP-MS) is a system for measuring metal elements, and is in fact the most sensitive method for elemental analysis. [11][12][13][14][15][16][17][18] A laser ablation inductively coupled plasma imaging mass spectrometry (LAICP-IMS) method has been developed for highly sensitive and quantitative surface analysis of metal elements in solid samples. [19][20][21][22][23][24] Managh et al reported tracking of gadolinium-labeled CD4 + T cells and gold-labeled human regulatory macrophages using LAICP-IMS.…”

Section: Introductionmentioning

confidence: 99%

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Cell tracking of chromium‐labeled mesenchymal stem cells using laser ablation inductively coupled plasma imaging mass spectrometry

Nakada

Kuroki

2019

Rapid Comm Mass Spectrometry

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Rationale Mesenchymal stem cells (MSCs) are widely used in regenerative medicine research. Evaluating the biodistribution of MSCs is important for determining whether the cells have reached the target tissue, and the time that the stem cells reside in each area is required to estimate the duration of efficacy. Methods A laser ablation inductively coupled plasma imaging mass spectrometry (LAICP‐IMS) method was developed for highly sensitive and quantitative surface analysis of metal elements for solid samples. We evaluated the usefulness of a cell‐tracking system with LAICP‐IMS to investigate the biodistribution of mouse mesenchymal stem cells (mMSCs) labeled with the natural composition of chromium (Cr) in mice. To prepare the dosing solution, mMSCs were incubated with both Na2CrO4 and fluorescent labeling solutions. The concentration of the cells was adjusted by vehicle solution at 2.0 to 2.5 × 107 cells/mL, and the dosing suspension of mMSCs was administered by intramuscular or intravenous injection to the mice. Results Thigh muscle sections after intramuscular injection of chromium‐ and fluorescence‐labeled mMSCs were analyzed by LAICP‐IMS and fluorescence microscopy, respectively. 52Cr mass spectrometry and fluorescence signals were detected in the same thigh muscle sections after administration of mMSCs. A half‐body section was also analyzed by LAICP‐IMS. 52Cr signals were mainly detected in the lungs. Conclusions The 52Cr signals were observed in sections through the thigh muscle and half body after intramuscular and intravenous administration, respectively, of Cr‐labeled mMSCs to mice. Our results suggest that LAICP‐IMS is a sensitive and useful technique to evaluate biodistribution in cell therapy research.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cell tracking of chromium‐labeled mesenchymal stem cells using laser ablation inductively coupled plasma imaging mass spectrometry

Nakada

Kuroki

2019

Rapid Comm Mass Spectrometry

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“…Using the calibration method (DIN 32645), the limits of detection and quantication for ergothioneine obtained with our HPLC/ICP-QQQ-MS method were 0.23 mg S per L and 0.80 mg S per L for m/z 32 / 48. The LOD was comparable to or slightly lower than those obtained for the determination of ergothioneine by LC/molecular MSMS 9,11 or other sulfur containing species by LC-ICP-QQQ-MS [23][24][25][26] and about 1 to 2 orders of magnitude lower compared to LC/UV methods 14,17,19 for the determination of ergothioneine. However, LOD and LOQ values obtained with aqueous ergothioneine standards, as is commonly reported, do not incorporate the inuence of matrix components, which were expected to be signicant in case of the complex matrix of the cell samples.…”

Section: Analytical Gures Of Meritmentioning

confidence: 63%

“…Thus in comparison with single quadrupole/reaction cell systems, the triple quadrupole system provides great selectivity and lower background counts, which translates into better detection limits for sulfur. 23 ICP-QQQ-MS has been so far mainly applied to the determination of sulfur in the eld of protein quantication, [23][24][25][26][27] with samples including solutions of sulfur-containing compounds, 23,25 DNAprotein cross-linking reaction products, 24 human plasma 25,26 and snake venom. 27 We present here a HPLC/ICP-QQQ-MS method for the quantitative determination of the health-relevant sulfur compound ergothioneine and its application to cell culture media and cell pellets.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of the sulfur-containing antioxidant ergothioneine by HPLC/ICP-QQQ-MS

Kroepfl

Marschall

Francesconi

et al. 2017

J. Anal. At. Spectrom.

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“…RP-SPE method is useful for desalting and fractionation for compound before quantification, however data show that the estimated weight of samples was influenced by significant amounts of non-targeted compounds (data not shown) may be the reason for observed low concentrations for 6, 7, 9 and 10 after quantification, compared to the data obtained for 8, which was purified by HPLC. Recent studies by Møller et al 67 for peptides in human plasma and Hermann et al 68 report for S-containing proteins shows the applicability of this method for accurate quantification of peptides. …”

Section: Biosynthetic Peptidesmentioning

confidence: 99%

Accurate quantification of modified cyclic peptides without the need for authentic standards

et al. 2016

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There is a growing interest in the use of cyclic peptides as therapeutics, but their efficient production is often the bottleneck in taking them forward in the development pipeline. We have recently developed a method to synthesise azole-containing cyclic peptides using enzymes derived from different cyanobactin biosynthetic pathways. Accurate quantification is crucial for calculation of the reaction yield and for the downstream biological testing of the products. In this study, we demonstrate the development and validation of two methods to accurately quantify these compounds in the reaction mixture and after purification. The first method involves the use of a HPLC coupled in parallel to an ESMS and an ICPMS, hence correlating the calculated sulfur content to the amount of cyclic peptide. The second method is an NMR ERETIC method for quantifying the solution concentration of cyclic peptides. These methods make the quantification of new compounds much easier as there is no need for the use of authentic standards when they are not available.

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Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection

Abstract: Quantification of pharmaceutical peptides in human plasma by RP-LC-ICP-MS and post column isotope dilution.

Cited by 15 publications

References 41 publications

Cell tracking of chromium‐labeled mesenchymal stem cells using laser ablation inductively coupled plasma imaging mass spectrometry

Cell tracking of chromium‐labeled mesenchymal stem cells using laser ablation inductively coupled plasma imaging mass spectrometry

Quantitative determination of the sulfur-containing antioxidant ergothioneine by HPLC/ICP-QQQ-MS

Accurate quantification of modified cyclic peptides without the need for authentic standards

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