Quantification of Physiologically Available Glycyrrhizin in Anti-Stress Herbal Formulations by Validated HPTLC Method

Abstract

In the present study a simple and novel high-performance thin-layer chromatography (HPTLC) method for quantitative determination of glycyrrhizin at stomach pH and its comparison with normal pH has been developed for marketed antistress liquorice root capsules and herbal tea. Chromatography was performed by using solvents including ethyl acetate:glacial acetic acid:methanol:water in proportion of 5:2:2:1, v/v/v/v as mobile phase. The developed plate was scanned and quantified densitometrically at absorption maxima 254 nm. The system was found to give compact spot for glycyrrhizin (Rf = 0.29 ± 0.001). The linearity relationship was described by the equation: Y = 2.103X + 25.289. Linearity range for glycyrrhizin was 100-1000 ng (r 2 = 0.996). The amount of glycyrrhizin was estimated by comparing the peak area of standard and the same was present in the crude extract of antistress herbal formulations. The content of glycyrrhizin in 1 g of drug material at normal pH (i.e. 6.8) of methanol was estimated as 7.50 % w/w and 6.05 % w/w in a dose of sample liquorice root and herbal tea, respectively. At pH 2.58 the glycyrrhizin concentration declined to 5.43 % w/w and 3.28 % w/w in sample liquorice root and herbal tea, respectively. The method was validated for precision, accuracy, recovery, robustness, specificity, detection and quantification limits in accordance with ICH guidelines.

Quantitative analysis of rutin, quercetin, naringenin, and gallic acid by validated RP- and NP-HPTLC methods for quality control of anti-HBV active extract of Guiera senegalensis

“…The developed HPTLC chromatograms are useful in identification of biomarkers in various herbal formulations by comparing the fingerprints with standards (Siddiqui et al. 2014 ). It is widely employed for the identification, purity testing, stability, dissolution or content uniformity of crude extracts of plant and animal origin, fermentation mix, drugs and excipients, including pharmaceutical, cosmetic and nutrient formulations (Alajmi et al.…”

Validated HPTLC Analysis for Estimation of Quercetin in Seeds of Anethum graveolens

“…In order to achieve a concentration of 200 ng/band, all three standard solutions were applied five times, showing the robustness of the developed method. [17][18][19]…”