Quantification of piroxicam and 5′-hydroxypiroxicam in human plasma and saliva using liquid chromatography–tandem mass spectrometry following oral administration

Calvo, Adriana Maria; Mulinari‐Santos, Gabriel; Dionísio, Thiago José; Marques, Maria Paula; Brozoski, Daniel; Lanchote, Vera Lúcia; Fernandes, Maria Helena; Faria, Flávio Augusto Cardoso de; Santos, Carlos

doi:10.1016/j.jpba.2015.12.042

Cited by 22 publications

(42 citation statements)

References 19 publications

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“…A dilution series from each stock solution (10 μg/mL)was used to construct standard curves. When not in use, solutions were stored in the dark at -20°C and all stages of the research were conducted under a sodium vapor lamp to avoid photodecomposition of naproxen, 6-O-desmethylnaproxen, esomeprazole, and the IS [ 7 , 8 ].…”

Section: Methodsmentioning

confidence: 99%

“…The concentrations of naproxen and its major metabolic, 6-O-desmethylnaproxen, were analyzed using an 8040 Triple Quadrupole Mass Spectrometer (Shimadzu, Kyoto, Japan)and piroxicam was used as an IS. Before performing the standard curve for naproxen, 6-O-desmethylnaproxen, esomeprazole, and the IS, the mobile phase was standardized to increase the reliability of the results that will be obtained from saliva samples in the PK studies [ 7 ]. As naproxen and esomeprazole ionize in different ways, only the PKs of naproxen and its major metabolite were investigated, as these are the drugs of most interest for pain control.…”

Section: Methodsmentioning

confidence: 99%

“…conducted under a sodium vapor lamp to avoid photodecomposition of naproxen, 6-O-desmethylnaproxen, esomeprazole, and the IS [7,8].…”

Section: Fundingmentioning

confidence: 99%

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Simultaneous separation of naproxen and 6-O-desmethylnaproxen metabolite in saliva samples by liquid chromatography–tandem mass spectrometry: Pharmacokinetic study of naproxen alone and associated with esomeprazole

et al. 2020

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Naproxen is a widely used non-steroidal anti-inflammatory drug for the control of postoperative inflammatory signs and symptoms in dentistry. Its association with esomeprazole has been widely studied and has yielded good results for the control of acute pain, even with the delayed absorption of naproxen owing to the presence of esomeprazole. To further understand the absorption, distribution, and metabolism of this drug alone and in combination with esomeprazole, we will analyze the pharmacokinetic parameters of naproxen and its major metabolite, 6-O-desmethylnaproxen, in saliva samples. A rapid, sensitive, and selective liquid chromatography-tandem mass spectrometric method for the simultaneous determination of naproxen and 6-O-desmethylnaproxen in saliva will be developed and validated. Sequential saliva samples from six patients will be analyzed before and 0

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Simultaneous separation of naproxen and 6-O-desmethylnaproxen metabolite in saliva samples by liquid chromatography–tandem mass spectrometry: Pharmacokinetic study of naproxen alone and associated with esomeprazole

et al. 2020

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“…In addition, according to WHO data and statistics from several countries, some NSAIDs are among the most widely used medicines in the world (3). One category of NSAIDs, enolic acids, is comprised of non-selective COX inhibitors such as piroxicam, which is mostly used to treat pain and inflammation (4,5).…”

Section: Introductionmentioning

confidence: 99%

“…In this study, we genotyped volunteers for the clinically relevant CYP2C9 polymorphisms, CYP2C9*2 and CYP2C9*3, using a rapid and efficient method, and performed pharmacokinetic analysis of piroxicam in five individuals who agreed to participate in the experiment. Saliva was chosen as a source of DNA and as the sample for pharmacokinetics analysis for a number of reasons including ease of collection, low cost, non-invasive and painless nature and low risk of infection (5,(9)(10)(11)(12). Therefore, the hypothesis of the present study is that saliva can be reliably used for pharmacogenetic and pharmacokinetic studies.…”

Section: Introductionmentioning

confidence: 99%

Pharmacogenetic and Pharmacokinetic Assays from Saliva Samples Can Guarantee Personalized Drug Prescription

et al. 2021

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Saliva is widely used for clinical and laboratory analysis. This study proposed to use DNA extracted from saliva for genotyping and pharmacokinetics of piroxicam. A fast and efficient genotyping method was used to determine relevant allelic variants of CYP2C9 (*2 and *3), since genetic factors can influence in non-steroidal anti-inflammatory drugs (NSAIDs) metabolization. DNA Extract All Reagents Kit® was used for DNA extraction and genotyping was performed using TaqMan® GTXpress™ Master Mix, SNP genotyping assays and a Viia7 Real-Time PCR system. Volunteers performed sequential collections of saliva samples before and after taking a single dose of piroxicam (0.25 to 72 h) which were used for pharmacokinetics assays. Piroxicam concentrations were analyzed using LC-MS/MS. Sixty-six percent of volunteers were ancestral homozygous (CYP2C9*1/*1), and 34% showed one or both polymorphisms. Of these 34%, 22 individuals showed CYP2C9*2 polymorphism, 8 CYP2C9*3, and 4 CYP2C9*2/*3. Piroxicam pharmacokinetics were performed in 5 subjects. Areas under the curve (AUC0-t(h*ng/mL)) for CYP2C9*1/*1, *1/*2 and *1/*3 were, respectively, 194.33±70.93, 166 and 303. Maximum concentrations (Cmax(ng/mL)) for these genotypes were respectively 6.46±2.56, 4.3 and 10.2. Saliva sampling was a very effective matrix for both pharmacogenetic and pharmacokinetic tests, ensuring the speed of the procedure and the well-being and agreement of the participants. Once having the knowledge about the slow and fast metabolizers, it is possible to make an adequate prescription in order to avoid the adverse effects of the medication and to guarantee greater analgesic comfort to the patients respectively.

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Simultaneous determination of piroxicam and norfloxacin in biological fluids by high‐performance liquid chromatography with fluorescence detection at zero‐order emission mode

et al. 2019

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A new highly sensitive high-performance liquid chromatographic method with fluorescence detection (HPLC-FLD) in zero-order emission mode was developed for the first time for the simultaneous determination of piroxicam (PRX) and norfloxacin (NRF) in biological fluids. The fluorescence detector wavelengths were set at 278 nm for excitation and zero-order mode for emission. The zero-order emission mode produced greater sensitivity for the measurement of both drugs than a fixed emission wavelength (446 nm). The new developed method was validated according to International Conference of Harmonization (ICH) guidelines. Linearity was found to be over concentration ranges 0.001-20 μg/ml and 0.00003-0.035 μg/ml for PRX and NRF, respectively. The limits of detection were 4.87 × 10 −4 and 1.32 × 10 −5 μg/ml for PRX and NRF, and the limits of quantitation were 1.47 × 10 −3 and 4.01 × 10 −5 μg/ml, respectively. The current fluorescence method was found to be more sensitive than most commonly used analytical methods and was successfully applied for simultaneous determination of PRX and NRF in biological fluids (serum and urine) with recoveries ranging from 91.67% to 100.36% for PRX and from 96.00% to 101.43% for NRF.

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Quantification of piroxicam and 5′-hydroxypiroxicam in human plasma and saliva using liquid chromatography–tandem mass spectrometry following oral administration

Cited by 22 publications

References 19 publications

Simultaneous separation of naproxen and 6-O-desmethylnaproxen metabolite in saliva samples by liquid chromatography–tandem mass spectrometry: Pharmacokinetic study of naproxen alone and associated with esomeprazole

Simultaneous separation of naproxen and 6-O-desmethylnaproxen metabolite in saliva samples by liquid chromatography–tandem mass spectrometry: Pharmacokinetic study of naproxen alone and associated with esomeprazole

Pharmacogenetic and Pharmacokinetic Assays from Saliva Samples Can Guarantee Personalized Drug Prescription

Simultaneous determination of piroxicam and norfloxacin in biological fluids by high‐performance liquid chromatography with fluorescence detection at zero‐order emission mode

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