Quantification of puerarin in plasma by on-line solid-phase extraction column switching liquid chromatography–tandem mass spectrometry and its applications to a pharmacokinetic study

Simultaneous determination of puerarin and its active metabolite in human plasma by UPLC-MS/MS: Application to a pharmacokinetic study

“…As well as HPLC, several studies applying liquid chromatography tandem mass spectrometry (LC-MS/MS) method have been reported for the quantification of puerarin tissue samples [9], plasma [10,18] and serum [19] in rats, or dog plasma [20]. Among them, Wang et al [20] presented the LC-MS/MS method with a comparably higher sensitivity of relatively LLOQ 0.39 ng/mL than other studies [9,10,18,19] with a LLOQ of 2-18.6 ng/mL for puerarin.…”

“…As well as HPLC, several studies applying liquid chromatography tandem mass spectrometry (LC-MS/MS) method have been reported for the quantification of puerarin tissue samples [9], plasma [10,18] and serum [19] in rats, or dog plasma [20]. Among them, Wang et al [20] presented the LC-MS/MS method with a comparably higher sensitivity of relatively LLOQ 0.39 ng/mL than other studies [9,10,18,19] with a LLOQ of 2-18.6 ng/mL for puerarin. Also, further studies provided information about the simultaneous determination of puerarin and daidzein in rat plasma by using LC-MS [2], UPLC-MS [21] or UPLC-MS/MS [22], serum [23] and urine [24] in humans by using HPLC.…”

An improved on-line solid phase extraction coupled HPLC–MS/MS system for quantification of Sifuvirtide in human plasma

“…The scheme of the on-line SPE HPLC-MS/MS system was similar to that in previous study [15,17] with minor modifications ( Fig. 1).…”

“…Via the improved system, Sifuvirtide in human plasma could be accurately quantified using a volume of only 20 L sample, and a better low limit of quantitation (LLOQ) of 6.1 ng/mL was obtained. Due to the use of an on-line extraction column and basic separation of the endogenous interferences from the analyte, there was no need for any complex sample pretreatment or the use of IS, the plasma can be directly injected into the system [5,17]. The total analysis turn-around time for one single sample, including on-line sample pretreatment and MS determination, was only 6.5 min.…”

Pharmacokinetic comparisons of puerarin, daidzin and the glucuronide metabolite of puerarin after administration of total flavonoid from Gegen alone and total flavonoid from Gegen combined with total saponin from Sanqi in rats under different physiological states

“…In addition, daidzin, another major isoflavonoid (isoflavonoid-O-glucoside) in Gegen, has recently been shown to have pharmacological effect on the cardiovascular system [8,9]. Therefore puerarin and daidzin are often used as marker constituents of Gegen in pharmacokinetic studies and a number of such studies have described the pharmacokinetics of puerarin and daidzin [10][11][12][13][14].…”