Quantification of pyrazinamide, isoniazid, acetyl‐isoniazid, and rifampicin by a high‐performance liquid chromatography method in human plasma from patients with tuberculosis

Abstract

The aim of this study was to establish and validate an alternative high‐performance liquid chromatography method for simultaneous quantification of pyrazinamide, isoniazid, acetyl‐isoniazid and rifampicin in plasma of patients under treatment for tuberculosis. The performed method was lineal (r2 > 0.99) in the range of 2.00–50.00 μg/mL for pyrazinamide, 0.50–20.00 μg/mL for both acetyl‐isoniazid and isoniazid, and 1.20–25.00 μg/mL for rifampicin. Precision and trueness were demonstrated with coefficient of variation < 15% and deviations < 15%, respectively, for quality controls samples. The lower limits of quantification were 2.00, 0.50, 0.50, and 1.20 μg/mL for pyrazinamide, isoniazid, acetyl‐isoniazid and rifampicin, respectively. The method was applied for the analysis of plasma from patients with tuberculosis. This method allowed ensuring reliable quantification of the target compounds and their pharmacokinetics parameters. In general, the mean values of maximum concentration of each antituberculosis drug were located within their respective reference therapeutic ranges. However, patients with sub‐therapeutic plasma concentrations of isoniazid and rifampicin were detected. This is the first analytical technique that simultaneously quantifies isoniazid, acetyl‐isoniazid, rifampicin, and pyrazinamide concentrations from plasma samples by high‐performance liquid chromatography with ultraviolet/visible. The proposed method could be applied for therapeutic drug monitoring and pharmacokinetics studies of the four compounds throughout the treatment of tuberculosis patients.

A Simple and Sensitive UPLC–UV Method for Simultaneous Determination of Isoniazid, Pyrazinamide, and Rifampicin in Human Plasma and Its Application in Therapeutic Drug Monitoring

“…Compared with the analytical methods reported in previous studies (Moussa et al, 2002;Allanson et al, 2007;Chen et al, 2010;Hernandez-Gonzalez et al, 2021), our developed UPLC-UV method improved retention times, reduced the volume of organic reagent, reduced the sample volume, and achieved symmetric peak shape. The total running time was 14 min, which was much shorter than that of the reported methods.…”

“…To determine plasma concentrations of the three firstline anti-TB drugs at the same time, a simple and accurate method is urgently required. Several analytical methods such as HPLC-MS/MS (Kim et al, 2015;Shah et al, 2016;Lei et al, 2019;Wu et al, 2019;Wang et al, 2020;Zheng et al, 20202020), HPLC-UV (Moussa et al, 2002;Allanson et al, 2007;Chen et al, 2010;Mourah et al, 2016;Hernandez-Gonzalez et al, 2021), andHPLC-FLD (El-Yazigi andRaines, 1992) have been reported to quantify these three drugs. Among these, HPLC-MS/MS is the most commonly used method, but it is difficult to widely promote in clinics because of its high costs and complex operation processes.…”

Multifunctional Fe/Cu Dual-Single Atom Nanozymes with Enhanced Peroxidase Activity for Isoniazid Detection and Levofloxacin Degradation

“…Currently, gas chromatography, fluorescence spectrophotometry, high-performance liquid chromatography, and electrochemistry have been developed for the detection of INH. All of these methods offer significant advantages, but most require expensive and time-consuming equipment and are costly.…”

A New Hybrid β-Cyclodextrin/PAMAM G.0 as an Electrochemical Sensor for Isoniazid Detection

“…Isoniazid (isonicotinic acid hydrazid) is an antibiotic drug for tuberculosis treatment [19][20]. Many methodologies have been applied to detect isoniazid, including uorimetry [21], chromatography [22], titrimetric [23], derivative spectrophotometry [24], indirect atomic absorption spectrometry [25], liquid chromatography-mass spectrometry [26], calorimetry [27] and electrochemical sensing, which are low-cost, simple, fast and selective analytical method [28].…”