Quantification of Reduced and Oxidized Glutathione in Whole Blood Samples by Capillary Electrophoresis

Serru, Valérie; Baudin, Bruno; Ziegler, F.; David, Jean‐Philippe; Cals, M J; Vaubourdolle, Michel; Mario, Nathalie

doi:10.1093/clinchem/47.7.1321

Cited by 69 publications

(39 citation statements)

References 17 publications

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“…The ratio of reduced GSH to oxidized GSH is an indicator of cellular health, with reduced GSH constituting up to 98% of cellular GSH under normal conditions. 27 The reduced-oxidized GSH ratio is used to evaluate oxidative stress status in biological systems, and alterations in this ratio have been demonstrated in aging, cancer, HIV replication, cardiovascular diseases, [28][29][30][31][32] and in neurodegenerative diseases, such as Parkinson disease and Alzheimer disease. 33 Thus, measuring the reduced-oxidized GSH ratio is an ideal way to assess potential therapeutics efficacy in maintaining cellular redox potential.…”

Section: Discussionmentioning

confidence: 99%

Genetic Instability in Lymphocytes is Associated With Blood Plasma Antioxidant Levels in Health Care Workers Occupationally Exposed to Ionizing Radiation

et al. 2016

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Earlier reports have suggested that exposure to radiation at workplace may induce cytogenetic abnormalities. However, the association between plasma antioxidants and the cytogenetic abnormalities in these patients has not been elucidated till now. Hence, the present study was undertaken to determine the relationship between the cytogenetic abnormalities, plasma antioxidant system, and the radiation exposure levels in men who were occupationally exposed to ionizing radiation. The study included 134 male volunteers, among whom 83 were occupationally exposed to ionizing radiation. Incidence of micronuclei and chromosomal aberration was assessed in lymphocytes. Total and reduced glutathione (GSH), total antioxidant capacity (TAC), superoxide dismutase (SOD), and lipid peroxidation were assessed in the plasma. The micronuclei frequency and chromosomal aberrations were significantly higher in the exposed group in comparison to the nonexposed group ( P < 0.01-0.0001). Similarly, GSH, TAC, and SOD in the blood plasma were significantly higher in the exposed group than the nonexposed group ( P < 0.01-0.0001). However, the level of malondialdehyde, which is an indicator of lipid peroxidation, did not differ significantly between both the groups. Importantly, radiation absorbed dose exhibited a positive correlation with the incidence of micronuclei in blood lymphocytes but not with chromosomal aberrations. This study shows that the susceptibility of peripheral blood lymphocytes to chromosomal damage is associated with plasma antioxidant levels. Furthermore, increased levels of blood plasma GSH, TAC, and SOD in occupationally exposed individuals could be an adaptive measure in response to oxidative stress to protect somatic cell genetic integrity.

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Section: Discussionmentioning

confidence: 99%

Genetic Instability in Lymphocytes is Associated With Blood Plasma Antioxidant Levels in Health Care Workers Occupationally Exposed to Ionizing Radiation

et al. 2016

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“…Levels of glutathione in the midbrain decrease prior to clinical symptoms of PD [117,163], impairing the function of GPx and promoting an increased oxidation [159]. Among the elderly, who are primarily affected by PD, glutathione levels are considerably decreased [164]. Therefore, PD patients may have an impaired ability to detoxify mercury and other xenobiotics due to glutathione depletion, leading to increased susceptibility.…”

Section: Glutathionementioning

confidence: 99%

Metals and Parkinson's Disease: Mechanisms and Biochemical Processes

Bjørklund

Stejskal²,

Urbina

et al. 2018

CMC

139

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Genetic background accounts for only 5 to 10% of the reported cases of Parkinson's disease (PD), while the remaining cases are of unknown etiology. It is believed that environmental factors may be involved in the causality of a large proportion of PD cases. Several PD genes are activated by xenobiotic exposure, and a link between pesticide exposure and PD has been demonstrated. Many epidemiological studies have shown an association between PD and exposure to metals such as mercury, lead, manganese, copper, iron, aluminum, bismuth, thallium, and zinc. This review explores the biological effects, the pathogenetic processes, genetic susceptibilities to metals as well as examining future strategies for PD treatment, such as chelation therapy.

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“…Intracellular and blood concentrations of GSH are in millimolar range, whereas the plasma concentration is in the micromolar range and accounts for approximately 0.4% of total blood GSH (Michelet et al 1995). Serru et al (2001) reported the following levels in whole human blood (expressed as mean ± SD) 486 ± 85 µmol/l for GSH, 68 ± 26 µmol/l for GSSG, and 553 ± 90 µmol/l for T-GSH. Giustarini et al (2003) measured the mean levels of 1384 µmol/l, 1378 ± 96 µmol/l, and 3.21 ± 0.87 µmol/l for T-GSH, GSH, and GSSG in whole blood, respectively.…”

Section: Meanmentioning

confidence: 99%

The evaluation of glutathione concentration in whole blood of Holstein dairy calves

et al. 2019

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The aim of this study was to evaluate the concentrations of total (T-GSH), oxidized (GSSG) and reduced glutathione (GSH) in blood of clinically healthy calves, to assess the effect of sex and age together with the type of feeding on its concentrations, and to assess the relationships between glutathione and metabolic indices. A total of 117 Holstein calves at the age of 1 day to 6 months were divided according to age and together with the type of feeding into 4 groups: colostrum and transition milk (n = 20), native milk (n = 39), milk replacer (n = 29) and total mixed ration (n = 29). Blood serum was used for the assessment of the metabolic profile of calves. The concentrations of individual forms of glutathione (mean ± SEM) were as follows: T-GSH: 789.1 ± 19.4 µmol/l, GSSG: 54.2 ± 4.3 µmol/l, GSH: 681.6 ± 15.3 µmol/l, and GSH/GSSG: 41.1 ± 5.9, with the coefficients of variability of 26.6%, 85.3%, 24.3% and 157.4%, respectively. Age and the type of feeding affected GSSG (P ≤ 0.01) and GSH/GSSG (P ≤ 0.01). The relationships between glutathione and biochemical parameters were mostly weak. T-GSH correlated with creatinine (P ≤ 0.05) and chlorides (P ≤ 0.05); GSSG correlated with albumin (P ≤ 0.01), creatinine (P ≤ 0.01), cholesterol (P ≤ 0.05), aspartate aminotransferase (P ≤ 0.01), alanine aminotransferase (P ≤ 0.01), gamma-glutamyltransferase (P ≤ 0.05) and calcium (P ≤ 0.05); GSH/GSSG ratio correlated with creatinine (P ≤ 0.01), cholesterol (P ≤ 0.05), aspartate aminotransferase (P ≤ 0.05), gamma-glutamyltransferase (P ≤ 0.01) and calcium (P ≤ 0.05). The evaluation of individual forms of glutathione helps in estimation of redox status in healthy animals and their monitoring can detect the stress extent.

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Quantification of Reduced and Oxidized Glutathione in Whole Blood Samples by Capillary Electrophoresis

Cited by 69 publications

References 17 publications

Genetic Instability in Lymphocytes is Associated With Blood Plasma Antioxidant Levels in Health Care Workers Occupationally Exposed to Ionizing Radiation

Genetic Instability in Lymphocytes is Associated With Blood Plasma Antioxidant Levels in Health Care Workers Occupationally Exposed to Ionizing Radiation

Metals and Parkinson's Disease: Mechanisms and Biochemical Processes

The evaluation of glutathione concentration in whole blood of Holstein dairy calves

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