Quantification of renin mRNA in various mouse tissues by a novel solution hybridization assay

Paul, Martin; Wagner, Dirk; Metzger, Rainer; Ganten, D; Lang, R. E.; Suzuki, Fujio; Murakami, Kazuo; Burbach, J. H. P.; Ludwig, Gerald M.

doi:10.1097/00004872-198803000-00010

Cited by 60 publications

(17 citation statements)

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“…As determined by quantitative PCR, renal reninmRNA expression was high with 1.74±0.2 pg renin-mRNA/ ,gg total renin RNA. This level is comparable to that reported in the literature with a range of 2.5-5.2 pg renin/,tg total RNA for rats and mice, respectively (20,21 ). In that PCR is extremely sensitive and allows quantification of specific mRNA expression from small tissue samples such as biopsy specimens, it may be of use to study changes in renal RAS gene expression in man under various in vivo conditions.…”

Section: Resultssupporting

confidence: 85%

“…The presence of renin mRNA in cardiac tissue, however, is still controversial. Whereas several investigators (20,21,24) have described specific mRNA expression by Northern blot and solution hybridization analysis in mouse and rat hearts, studies by Ekker et al (25) and Iwai and Inagami (18) were unable to detect cardiac renin mRNA. It is unclear at present ifthe apparent discrepancy is due to species differences, experimental conditions or differential regulation of renin.…”

Section: Resultsmentioning

confidence: 99%

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Gene expression of the renin-angiotensin system in human tissues. Quantitative analysis by the polymerase chain reaction.

Paul

Wagner²,

Dzau³

1993

J. Clin. Invest.

227

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Activation of tissue-specific gene expression of the components of the renin-angiotensin system (RAS) in humans may play an important role in cardiovascular regulation and pathophysiology. Studies of human tissue RAS expression, however, have been limited by the lack of availability of sufficient amounts of fresh human tissues and a sensitive method for detecting specific mRNAs. To demonstrate the presence of components of local RASs in humans we used the polymerase chain reaction (PCR) after reverse transcription to detect renin-, angiotensinogen-, and angiotensin-converting enzyme-mRNA in small quantities of human tissues. Results indicated that all components of the RAS were widely expressed in human organ samples. In order to study changes of gene expression in small tissue samples (e.g., renal biopsies) obtained from patients, we established a competitive PCR assay for quantification ofrenin, using a 155-basepair deletion mutant of the human renin cDNA as an internal standard. Renin-mRNA concentration was quantitated in the kidney (1.74±0.2 pg renin/,gg total RNA), adrenal gland (1.15±0.15 pg renin/gg total RNA), placenta (0.7±0.1 pg renin/,gg total RNA), and saphenous vein (0.02±0.01 pg renin/gg total RNA). The method described here may serve as a highly sensitive tool to quantify alterations in gene expression in man under various pathophysiologic conditions. This study should provide the methodological basis for future studies of tissue RAS in human physiology and disease. (J. Clin.

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Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Gene expression of the renin-angiotensin system in human tissues. Quantitative analysis by the polymerase chain reaction.

Paul

Wagner²,

Dzau³

1993

J. Clin. Invest.

227

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“…Quantification of Messenger RNA The regulation of a candidate gene in diverse tissues can be studied by the determination of its RNA transcript by means of hybridization analysis techniques such as Northern blots, 19 slot blots, 20 solution hybridization, 21 and SI protection assay. 22 Such methods can reveal the levels of a specific mRNA in response to certain stimuli.…”

Section: Messenger Rna Expression: Tissue Specificity and Regulationmentioning

confidence: 99%

Role of molecular biology in hypertension research. State of the Art lecture.

et al. 1989

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In this article we will examine the potential impact of molecular biology on hypertension research. We will review the available molecular techniques, which include gene cloning, transient and stable expressions, as well as the use of transgenic animals. To facilitate our discussion, we will focus primarily on research of the renin gene. Renin provides a useful model that illustrates the power of biotechnology in providing detailed structural and biochemical information on a complex protein that exists in low quantities in vivo. Studies of its messenger RNA and gene expression have resulted in an improved understanding of the biology of the renin system and in generating new hypotheses. These approaches can be generalized to studies of other vasoactive hormones, contractile protein, and other gene products related to cardiovascular regulation. To elucidate the role of a specific gene in genetic hypertension, we will discuss the use of genetic markers in cosegregation or linkage analysis. Finally, we will examine the potential of transgenic animals in the study of regulation of gene expression in the whole animal and the contribution of selective genes to hypertension. We believe that molecular biology complements the biochemical and physiological approaches and provides new opportunities for furthering our concept of hypertension mechanisms. (Hypertension 1989;13:731-740) B lood pressure is regulated by multiple control mechanisms. Abnormalities in the regulation of one or several of these control systems may result in a transient or a sustained elevation in blood pressure. For instance, renal, endocrine, neurogenic, cardiogenic, and vascular mechanisms have each been demonstrated to participate in the development of experimental hypertension as well as in the pathogenesis of secondary forms of human hypertension. However, the etiology and pathogenetic mechanisms of essential hypertension are still enigmatic. Although many altered physiological responses and biochemical parameters have been reported, both in patients with essential hypertension and in animal models of genetic hypertension, it remains unclear which of the various reported changes are primary (i.e., causative) and which are secondary (i.e., the result of hypertension). Physiological and biochemical events are usually modulated by multiple related

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“…As the local production of AOGEN (22,60,(139)(140)(141), renin (22,(142)(143)(144) and ACE (14,23) mRNA, as well as the presence of ANG II receptors (145-148) was confirmed in rat and human myocardium, the existence of a local RAS in the heart was postulated (149)(150)(151).…”

Section: Heartmentioning

confidence: 99%

The Role of the Renin-Angiotensin System in Cardiovascular Disease.

et al. 1994

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Recent findings have increased our understanding of the role of the renin-angiotensin system in cardiovascular diseases. This was possible by the progress made in molecular biology and the import of this methodology into cardiovascular research by several groups in the past few years. Studies by these groups accumulated evidence especially for the existence and physiological importance of organ-based renin-angiotensin systems and their involvement in the pathogenesis of hypertension and the accompanying disorders of the heart, the kidney, and the vessels. This Review will focus mainly on the relevance of the local renin-angiotensin systems for cardiovascular diseases and the value of transgenic animals as models to analyse these systems (Hypertens Res 1994; 17: 1-16).

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Quantification of renin mRNA in various mouse tissues by a novel solution hybridization assay

Cited by 60 publications

References 0 publications

Gene expression of the renin-angiotensin system in human tissues. Quantitative analysis by the polymerase chain reaction.

Gene expression of the renin-angiotensin system in human tissues. Quantitative analysis by the polymerase chain reaction.

Role of molecular biology in hypertension research. State of the Art lecture.

The Role of the Renin-Angiotensin System in Cardiovascular Disease.

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