Quantification of residual ophthalmic viscosurgical device after irrigation/aspiration in experimental cataract surgery in vitro

Comparison of the Residual Amount of an Ophthalmic Viscosurgical Device Among Different Types of Intraocular Lens Implants in vitro

“…After IOL insertion and subsequent I/A, the distribution of residual OVD in the capsular bag was objectively observed using ultraviolet (UV) light. 16 In this previous study, photographs of the labeled residual OVD in IOL obtained using UV light revealed that OVD tended to remain at the center of the optic of the single-piece IOL. In addition, a comparison of single-piece IOLs and three-piece IOLs using an indirect quantification method devised by us revealed that OVD tended to remain more in the single-piece IOLs.…”

“…The specimen was prepared using an operating microscope (OPMI-1 FR, Carl Zeiss Meditec, AG, Jena, Germany), irradiated with UV light from behind, and observed in Miyake–Apple view 18 using a digital camera (FDR-AX 30; SONY Co., Ltd., Tokyo, Japan) ( Figure 1A ). In our previous study, 16 the labeled OVDs were visualized under UV light, and we observed that parts of the capsular bag and the single-and three-piece IOLs were prone to OVD retention. In the present study, we used the same methods to visualize the retention tendency of various single-piece IOLs ( Figure 1B ).…”

“…In the present study, using a method for indirectly quantifying OVD by labeling the OVD with known chemical substances, based on our previous study, 16 we compared the amounts of residual OVDs in single-piece IOLs made by five different manufacturers. We observed that the distribution tendency of residual OVDs in the sac differed depending on the IOL type, and the amounts of residual OVDs in the five types of single-piece IOLs were different even for the same type of IOLs.…”

“…The experiments were performed using previously published methods. 16 Fluorescent silica particles (micromod Partikeltechnologie GmbH, Rostock, Germany) were used as OVD labels. Fluorescein isothiocyanate (excitation wavelength, 485 nm; emission wavelength, 515 nm) was encapsulated in silica shells with a 30-nm diameter and dispersed in a buffer solution with a density of 1 g/mL at a concentration of 100 mg/mL.…”

“…Therefore, we developed a method to quantify the residual amount of OVD in the capsule. 16 In vitro cataract surgery was performed using OVD mixed with a fluorescent dye labeled with fluorescent silica particles. After IOL insertion and subsequent I/A, the distribution of residual OVD in the capsular bag was objectively observed using ultraviolet (UV) light.…”