Quantification of serum immunoglobulin in rainbow trout Salmo gairdneri under various environmental conditions

Abstract

The concentration of serum immunoglobulin in different groups of rainbow trout Salrno gairdneri was measured by means of a single radial immuno&ffusion test. The normal IgM concentration was 3.3 mg ml-' in a group of free-living trout. Hypergamrnaglobulinaemia was found in PKDinfected fish and in fish from a trout farm with VHS and ERM, while the IgM concentration was low in sera from fish living under aquarium con&tions. Inhvidual variations were very pronounced. The purity of reference preparations and the specificity of antisera used were examined by crossed in~munoelectrophoresis.

Association of plasma IgM with body size, histopathologic changes, and plasma chemistries in adult Pacific herring Clupea pallas

“…This is the first report of plasma IgM levels in Pacific herring. Serum IgM concentrations have been reported for several other species: Oncorhynchus mykiss, 0.6 to 9.3 mg rnl-' ) and 3.3 mg ml-' (Olesen & Jsrgensen 1986); Salmo trutta, 7.3 mg ml-' (Ingram & Alexander 1979); Oncorhynchus keta, 0.63 to 0.72 mg rnl-' (Kobayashi et al 1982); and Scophthalmus maximus, 3.35 to 11.14 mg ml-' (Estevez et al 1995). The IgM component of total serum protein in fish has been reported as 6.4 % (Mochida et al 1994), 10 % (Ingram & Alexander 1979), and 7.5 to hoferi infection, a variety of nonspecific histologic 18% (Estevez et al 1995).…”

“…Circulating ilnmunoglobulin levels in various species of fish vary with body size (Fuda et al 1991, Estevez et al 1995, temperature (Wilson & Warr 1992), season (Yamaguchi et al 1981, Zapata et al 1992, and pathogenic organisms (Olesen & Jsrgensen 1986, Magnadottir & Gudmundsdottir 1992; however, evaluation of the multiple interactive effects of body size, plasma chemistries and pathological changes upon circulating imn~unoglobulins in fish is lacking. Also, little data are available on plasma IgM in free-ranging fish and the effects of disease on plasma IgM levels in wild populations.…”

Alterations in total protein, IgM and specific antibody activity of male and female sea bass (Dicentrarchus labraxL., 1758) sera following injection with killedVibrio anguillarum

“…Most studies on sea bass have been devoted to non-specific defence mechanisms and to anatomical descriptions of lymphoid organs (Romestand & Trilles, 1984;Angelidis, 1987;Deschaux et al, 1987;Meseguer et al, 1991Meseguer et al, & 1993, whereas others deal with diagnostic techniques (Romestand et al, 1993). The occurrence of specific antibodies resulting from natural infections or experimental injections has been well studied (Anderson & Klontz, 1975;Cossarini-Dunier, 1986;Olensen & Vestergard Jorgensen, 1986;Israelsson et al, 1991;Maggnadottir & Gudmundsdottir, 1992;Estévez et al, 1994) but there have been few studies on proportional changes in total immunoglobulin (IgM) with respect to total protein or specific antibodies (Olensen & Vestergard Jorgensen, 1986;Maggnadottir & Gudmundsdottir, 1992). Studies focusing on the production of monoclonal antibodies (MAb) to sea bass immunoglobulins Romestand et al, 1995) have permitted the development of a sensitive ELISA method (Bourmaud, 1994) allowing natural or induced IgM-like and specific antibody production to be monitored.…”

Isolation and characterization of immunoglobulin M of Asian sea bass, Lates calcarifer and its level in serum

“…The mean value of the IgM concentration in the sera of ten healthy sea bass was 5.4 ±1.8 mg ml -1 , which is quite similar to the serum IgM concentration of cod, 5.63 mg ml -1 [39] and 6.48 mg ml -1 in turbot [34]. However, there is a lot of variation in serum Ig concentration reported in other fish species such as sheepshead-3.07 mg ml -1 [36], rainbow trout-3.3 mg ml -1 [40], paddlefish-17.2 mg ml -1 [41], yellowtail-0.96 mg ml -1 [42] and Atlantic salmon-0.8 mg ml -1 [4]. This variation in IgM levels may be due to the difference of age between fishes or due to different environmental factors such as different conditions where the fishes were reared.…”