2005
DOI: 10.1002/rcm.1917
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Quantification of sifuvirtide in monkey plasma by an on‐line solid‐phase extraction procedure combined with liquid chromatography/electrospray ionization tandem mass spectrometry

Abstract: A simple, automated and rapid method has been developed for the determination of a novel antiviral peptide sifuvirtide in monkey plasma. Raw plasma samples were directly loaded onto an on-line solid-phase extraction (SPE) column, which removes the time-consuming and laborious sample pretreatment. Following a timed valve-switching event, the analyte was eluted on-line to a reversed-phase high-performance liquid chromatography (RP-HPLC) column and subsequently introduced into a linear ion trap mass spectrometer,… Show more

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Cited by 28 publications
(31 citation statements)
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“…The interbatch CV ranged from 2.9% to 8.8%, and the intra-batch CV did not exceed 10.5%. The stability of sifuvirtide in solution and in plasma samples was also evaluated [10] . The areas ratios of SRM chromatography spectra of sifuvirtide versus IS were investigated in stock solution kept in 4 °C for approximately 15 d and in plasma samples left at 15 °C overnight (12 h-14 h).…”
Section: Methods Validationmentioning
confidence: 99%
See 1 more Smart Citation
“…The interbatch CV ranged from 2.9% to 8.8%, and the intra-batch CV did not exceed 10.5%. The stability of sifuvirtide in solution and in plasma samples was also evaluated [10] . The areas ratios of SRM chromatography spectra of sifuvirtide versus IS were investigated in stock solution kept in 4 °C for approximately 15 d and in plasma samples left at 15 °C overnight (12 h-14 h).…”
Section: Methods Validationmentioning
confidence: 99%
“…The profiles of absorption, distribution, metabolism and excretion of sifuvirtide have been characterized in rats and monkeys by using radioactive 125 I-sifuvirtide combined with radioactivity detection of samples derived from trichloroacetic acid precipitation or size-exclusion chromatography. For the requirement of clinical pharmacokinetic (PK) study, we have developed an advanced and validated on-line solid-phase extraction procedure combined with liquid chromatography tandem mass spectrometry (SPE-LC/MS/MS) approach for determination of non-labeled sifuvirtide in monkey plasma [10] . In the present study, an in vitro fusion inhibitory experiment was used to determine the 50% inhibitory concentration (IC 50 ) of sifuvirtide.…”
Section: Introductionmentioning
confidence: 99%
“…Although the use of these techniques for quantitative bioanalytical assays of large peptides (>2000 Da) has recently been described more frequently [1][2][3][4][5][6][7][8][9][10][11][12], the application for absolute protein quantification (>6000 Da) is still not widespread [13][14][15]. However, with the increasing importance of peptides and proteins in different fields, e.g.…”
Section: Introductionmentioning
confidence: 98%
“…Stable isotopically labeled ISs are most suitable for LC-MS quantification, but are, unfortunately, expensive, especially for large compounds. The use of structural analogs can be a very useful alternative [3,7,11], but a suitable structural analog of the peptide of interest may be hard to find. For the quantification of intact proteins it is even more difficult to find a suitable IS.…”
Section: Introductionmentioning
confidence: 99%
“…Extraction methods are thus needed to remove endogenous proteins and concentrate the biotherapeutic and cognate catabolites of interest. Methods include protein precipitation, solid-phase extraction, and affinity purification (Ji et al, 2003;Dai et al, 2005;Ackermann and Berna, 2007;Heudi et al, 2008;Ezan et al, 2009;Lu et al, 2009;Liu et al, 2011b;Li et al, 2012). The first two methods are more generally applied to peptides and low-molecular-weight proteins and will not be addressed in detail here.…”
Section: Workflows For Studying Protein Biotherapeutic Biotransformationmentioning
confidence: 99%