Fertilizer is one of the main inputs in agriculture. Moving towards soil sustainability, this study intended to determine the effects of different types of biofertilizer on selected peat soil properties and to evaluate the growth response of Pak Choy planted in the peat soils. In this study, the peat soil was taken at selected area recommended by Pertubuhan Peladang Kawasan (PPK) in Tangkak, Johor, Malaysia. The treatments consist of three (3) different types of biofertilizer specifically Biofertilizer A (T1), Biofertilizer B (T2), Biofertilizer C (T3) and NPK 15:15:15 (T0) as a control with four replications, applied according to the recommended rate specified for each fertilizer. The treatments were arranged using randomized complete block design (RCBD) in a greenhouse. The selected soil properties (soil pH, soil moisture content, soil available P, soil exchangeable K, Ca and Mg) was analyse during the initial and after harvesting period whilst the growth response (number of leaves, plant height) of Pak Choy was monitored weekly. Findings showed that there are significant differences in soil pH and soil available P between treatments. The soil pH is significantly improved upon application of biofertilizers, specifically the Biofertilizer B and Biofertilizer C. Additionally, other parameters such as K, Ca and Mg indicates a positive increment upon biofertilizer application as compared to control. As such, it can be concluded that there is a positive effect of different types of biofertilizer application on selected peat soil properties and the growth response of Pak Choy planted in the peat soils.