Quantification of substratum contact required for initiation of Colletotrichum graminicola appressoria

Apoga, Dace; Barnard, J. A.; Craighead, Harold G.; Hoch, H. C.

doi:10.1016/j.fgb.2003.10.001

Cited by 34 publications

(27 citation statements)

References 57 publications

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“…Botrytis cinerea, Discula umbrinella, Phyllosticta ampelicida and Bipolaris sorokiniana, in which there is an initial phase of adhesion mediated by hydrophobic interactions before the secretion of an adhesive ECM [7,9,29,43,44]. This mechanism of adhesion is clearly different to that of some other fungal pathogens in which pre-formed adhesive is secreted ARTICLE IN PRESS on contact with a surface e.g.…”

Section: The Role Of the Spore Coat In Pathogenicitymentioning

confidence: 99%

“…Most fungi will germinate without a requirement for contact with a hard surface, although some fungi, e.g. Phyllosticta species and C. graminicola require initial attachment for germination as well as appressorium formation [7][8][9]17]. …”

Section: The Role Of the Spore Coat In Pathogenicitymentioning

confidence: 99%

“…For C. graminicola, contact and/or adhesion of germtubes to a substratum is needed for appressorium formation, and it has been shown that 44 mm of continuous contact of the germ-tubes with a hydrophobic substratum is required in this case [7]. However, protease-treated spores of C. lindemuthianum still germinated and produced adhesive ECM glycoproteins, which suggests that these processes are not sufficient to trigger appressorium formation without the early contact of the spore with a hard surface.…”

Section: An Intact Spore Coat Is Required For Appressorium Formationmentioning

confidence: 99%

“…Whether the surface is hydrophilic or hydrophobic can also affect germination and/or appressorium formation, e.g. C. lagenarium germinates more effectively on hydrophilic surfaces [6], whereas C. graminicola and Phyllosticta amelicida germinate and produces appressoria preferentially on hydrophobic surfaces [7][8][9]. In some cases, chemical cues are also required for appressorium formation e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Phyllosticta ampelicida and other Phyllosticta species [16,17], C. graminicola [7,8] and C. trifolii [18]) and appressorium development (e.g. M. grisea [19]; C. graminicola [7,8]). It has also been shown that adhesion of C. graminicola spores is required for efficient infection of corn leaves [20].…”

Section: Introductionmentioning

confidence: 99%

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The spore coat of the bean anthracnose fungus Colletotrichum lindemuthianum is required for adhesion, appressorium development and pathogenicity

Rawlings

O’Connell²,

Green

2007

Physiological and Molecular Plant Pathology

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The spores (conidia) of the bean anthracnose fungal pathogen, Colletotrichum lindemuthianum, adhere to the aerial parts of plants to initiate the infection process. In previous studies we have shown that the Colletotrichum spores are surrounded by a fibrillar spore coat, comprising several major glycoproteins. Previous evidence showed that a monoclonal antibody (UB20) that recognised these glycoproteins was able to inhibit adhesion of spores to a hydrophobic surface. In this paper we have further studied the role of the spore coat in adhesion, germination and fungal development by studying the effects of UB20 and protease treatment of spores. The latter treatment has previously been shown to remove the spore coat. Spores germinate on glass, polystyrene and water agar, however, appressoria only develop on glass or polystyrene, showing a requirement for a hard surface. Removal of the spore coat with protease inhibits adhesion at 30 min, before the secretion of ECM glycoproteins. Protease treatment also inhibits the development of appressoria and reduces pathogenicity on leaves. Incubation of spores with the MAb UB20 inhibits adhesion at 30 min, but does not affect appressorium formation or pathogenicity. The results suggest that an intact spore coat has two functions; it is required for adhesion to a hydrophobic surface and for the detection of a hard surface necessary for appressorium formation. We suggest that contact with a hard surface, rather than adhesion, is the key event leading to appressorium formation. r

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Section: The Role Of the Spore Coat In Pathogenicitymentioning

confidence: 99%