Quantification of the binding affinity of a specific hydroxyapatite binding peptide

Weiger, Michael C.; Park, Jung Jin; Roy, Marc D.; Stafford, Christopher M.; Karim, Alamgir; Becker, Matthew L.

doi:10.1016/j.biomaterials.2010.01.012

Cited by 56 publications

(53 citation statements)

References 48 publications

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“…Finally, colonies of infected E. coli cells are grown (each colony only multiplies one type of phage) and the amino-acid sequence of the binding peptides is deduced by evaluating the sequence of the phage DNA ( Figure 3). In material science, phage-display technology has been used for the selection of amino-acid sequences that bind specifically to various inorganic (GaAs (50), AlGaAs (50), valerite (51), calcite (52), hydroxyapatite) and polymeric materials (53)(54)(55)(56).…”

Section: Phage Display Assisted Design Of Bioconjugatesmentioning

confidence: 99%

Peptide-Polymer Conjugates as Model Systems To Explore the Functional Space of Precision Polymers

Brummelhuis

Wieczorek

Wilke

et al. 2014

ACS Symposium Series

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Section: Phage Display Assisted Design Of Bioconjugatesmentioning

confidence: 99%

Peptide-Polymer Conjugates as Model Systems To Explore the Functional Space of Precision Polymers

Brummelhuis

Wieczorek

Wilke

et al. 2014

ACS Symposium Series

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“…Phage display was used to discover peptide sequences with selective affinity for HA [90–93] or β-TCP [94]. In the latter study, a β-TCP binding domain was engineered onto EGF and the fusion protein was then anchored onto β-TCP disks.…”

Section: Functionalizing Bone-mimetic Scaffolds With Growth Factorsmentioning

confidence: 99%

Taking cues from the extracellular matrix to design bone-mimetic regenerative scaffolds

et al. 2016

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There is an ongoing need for effective materials that can replace autologous bone grafts in the clinical treatment of bone injuries and deficiencies. In recent years, research efforts have shifted away from a focus on inert biomaterials to favor scaffolds that mimic the biochemistry and structure of the native bone extracellular matrix (ECM). The expectation is that such scaffolds will integrate with host tissue and actively promote osseous healing. To further enhance the osteoinductivity of bone graft substitutes, ECM-mimetic scaffolds are being engineered with a range of growth factors (GFs). The technologies used to generate GF-modified scaffolds are often inspired by natural processes that regulate the association between endogenous ECMs and GFs. The purpose of this review is to summarize research centered on the development of regenerative scaffolds that replicate the fundamental collagen-hydroxyapatite structure of native bone ECM, and the functionalization of these scaffolds with GFs that stimulate critical events in osteogenesis.

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“…In [43] it was found that phosphorylation (i.e., the introduction of C-site-binding groups) of a peptide had no effect on the affinity in case of chromatographic HA, but had a greater influence in the case of a more bone-like mineral. When phage display (i.e., a biological method to screen large libraries) was used to identify HAbinding linear peptides [4,44], several sequences, including STLPIPHEFSRE, VTKHLNQISQSY and SVSVGMKPSPRPGGGK emerged. In the last case, the SVSV fragment was mainly responsible for the interaction, whereas the MKPSP fragment apparently provided a conformational-dependent component that enhanced the peptide's binding, but by itself did not show any specific affinity.…”

Section: Interaction Mechanismsmentioning

confidence: 99%

Isolation and purification of recombinant proteins, antibodies and plasmid DNA with hydroxyapatite chromatography

Hilbrig

Freitag

2011

Biotechnology Journal

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Hydroxyapatite and related stationary phases increasingly play a role in the downstream processing of high-value biological materials, such as recombinant proteins, therapeutic antibodies and pharmaceutical-grade plasmid DNA. Chromatographic hydroxyapatite is an inorganic, ceramic material identical in composition, if not in structure, to calcium phosphate found in human bones and teeth. The interaction of hydroxyapatite with biomacromolecules is complex and highly dynamic, which can make predicting performance difficult, but also allows the design of very selective isolation processes. This review discusses the currently commercially available chromatographic materials, different retention mechanisms supported by these materials and differential exploitation for the design of highly specific isolation procedures. The state of the art of antibody purification by hydroxy-and fluoroapatite is reviewed together with tested routines for method development and implementation. Finally, the isolation of plasmid DNA is discussed, since the purification of DNA therapeutics at a sufficiently large scale is an emerging need in bioprocess development and perhaps the area in bioseparation where apatite chromatography can make its most important contribution to date.

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Quantification of the binding affinity of a specific hydroxyapatite binding peptide

Cited by 56 publications

References 48 publications

Peptide-Polymer Conjugates as Model Systems To Explore the Functional Space of Precision Polymers

Peptide-Polymer Conjugates as Model Systems To Explore the Functional Space of Precision Polymers

Taking cues from the extracellular matrix to design bone-mimetic regenerative scaffolds

Isolation and purification of recombinant proteins, antibodies and plasmid DNA with hydroxyapatite chromatography

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