Quantification of total sheep IgE concentration using anti-ovine IgE monoclonal antibodies in an enzyme immunoassay

Shaw, R.J.; McNeill, Margaret M.; Gatehouse, T.K.; Douch, P.G.C.

doi:10.1016/s0165-2427(97)00010-x

Cited by 33 publications

(23 citation statements)

References 12 publications

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“…Considerável variação na expressão da imunidade dos ovinos aos helmintos gastrintestinais foi observada em outros estudos (Shaw et al, 1997;Gómez-Muñhoz et al,1998). No presente trabalho, a variabilidade pode ter sido evidenciada devido à infecção natural, à baixa amostragem do lote e às diferenças de idade e de raça entre os animais.…”

Section: Resultsunclassified

Uso De Marcadores Parasitológicos E Imunológicos Na Seleção De Ovelhas Resistentes Às Parasitoses Gastrintestinais

Rosalinski-Moraes

Sotomaior

Schmidt

et al. 2011

AVS

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RESUMO:Este trabalho foi realizado com objetivo de avaliar a possibilidade de identificar ovelhas resistentes e susceptíveis às parasitoses por meio de um marcador parasitológico (direto), de marcadores imunológicos (indiretos) ou pela associação entre ambos. Vinte ovelhas foram acompanhadas por coletas mensais de sangue e fezes no período de novembro de 1998 a maio de 1999. O marcador parasitológico utilizado foi o número de ovos de helmintos por grama de fezes (OPG); os marcadores imunológicos foram o número de eosinófilos sanguíneos, imunoglobulinas G (IgG) e E (IgE) anti-Haemonchus contortus. As ovelhas puderam ser classificadas como resistentes ou susceptíveis pelo OPG. Pelo o número de eosinófilos ou pela IgE específica utilizados concomitantemente com o OPG foi possível identificar ovelhas resistentes e susceptíveis ao parasitismo gastrintestinal. O número de eosinófilos sanguíneos foi o único marcador imunológico que permite de classificar as ovelhas indiretamente nos mesmos grupos obtidos pelo o OPG. Palavras-chave: eosinófilos; imunoglobulina G; imunoglobulina E; resistência aos parasitos USAGE OF PARASITOLOGICAL AND IMMUNOLOGICAL MARKERS FOR THE SELECTION OF EWES RESISTANT TO GASTROINTESTINAL PARASITESABSTRACT: This work was conducted to verity the possibility to identify adult sheep that are resistant to parasites by using a parasitological marker (direct), immunological markers (indirect) or by the association of both types of markers. Twenty ewes were sampled monthly for blood and faeces, from July of 1998 to June of 1999. Faecal egg counts (FEC) was chosen as parasitological marker. The number of peripheral eosinophils, IgE and IgG anti-Haemonchus contortus were used as immunological markers. Sheep could be classified as resistant or susceptible by FEC. Both peripheral eosinophils and specific IgE data could be joined to FEC in order to identify resistant or susceptible animals. The number of peripheral eosinophils was the only immunological maker that was able to classify high and low FEC ewes in two different groups.

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Section: Resultsunclassified

Uso De Marcadores Parasitológicos E Imunológicos Na Seleção De Ovelhas Resistentes Às Parasitoses Gastrintestinais

Rosalinski-Moraes

Sotomaior

Schmidt

et al. 2011

AVS

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“…Studies investigating ovine IgE antibody responses have been hampered by a lack of speci®c antibodies to ovine IgE. Only recently have systems been developed that allow us to measure ovine IgE responses (Shaw et al 1996(Shaw et al , 1997(Shaw et al , 1998Kooyman et al 1997;Huntley et al 1998Huntley et al , 1999.…”

Section: Discussionmentioning

confidence: 99%

“…Total IgE was quantitated as units per millilitre of serum using the enzyme-linked immunosorbent assay (ELISA) described by Shaw et al (1997). Levels of T. colubriformis larval excretory/secretory antigen-speci®c IgE were determined as optical density (OD) values using the ELISA described by Shaw et al (1998).…”

Section: Serology Testsmentioning

confidence: 99%

Immunisation of lambs with drug-abbreviated Haemonchus contortus infections: protection against homologous and heterologous challenge

et al. 2000

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Haemonchus contortus is known as a prolific parasite, producing high numbers of eggs. It could therefore be used as a cheap source of larvae for immunisation of lambs. The value of immunisation would be improved if the immunity produced gave protection against not only homologous but also heterologous infections. Because antibody cross-reactivity between Haemonchus and Ostertagia has been well established, we wanted to know whether drug-abbreviated infections of H. contortus would stimulate production of antibodies that would cross-react with Trichostrongylus colubriformis. The results obtained from these trials indicated that H. contortus drug-abbreviated infections produced significant immunity against not only Haemonchus but also O. circumcincta. Immunisation increased the level of immunoglobulin E (IgE) and IgE-specific antibodies against T. colubriformis, but the differences between experimental and control animals were not statistically significant. Significantly higher levels of IgG-specific antibodies against T. colubriformis were observed.

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“…Total IgE was quantitated as units per milliliter of serum using the enzyme-linked immunosorbent assay (ELISA) described by Shaw et al (1997). Antigen speci®c IgE was determined by an optical density (OD) ELISA (Shaw et al 1998) using excretory-secretory larval T. colubriformis antigen.…”

Section: Serologymentioning

confidence: 99%

“…To determine how the immune system of sheep would respond to such immunization, we studied total IgE and speci®c IgE and IgG, as these immunoglobulins had been shown to be stimulated or somehow involved in a protective immune response (Baker andGershwin 1992, 1993;Hagan 1993;Pritchard 1993;Douch et al 1994;Shaw et al 1996Shaw et al , 1997Shaw et al , 1998.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulation of lambs following treatment with a proteasome preparation from infective larvae of Trichostrongylus colubriformis

Stankiewicz

Hadaś

2000

Parasitology Research

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Proteinases are known to be capable of prolonging the survival of endoparasites in a host. We were therefore interested in knowing whether immunization of lambs against a proteasome (multisubunit proteinases) preparation obtained from Trichostrongylus colubriformis infective third-stage larvae (L3) would have any effect on the immune response to a single challenge infection with the same organism. A total of 21 penned lambs aged 8 months were divided into 3 equal groups. Group 1 was immunized on three occasions with increasing amounts of a proteasome-enriched fraction obtained from infective L3. Group 2 was given a similar amount of protein from the initial supernatant of homogenized larvae. Group 3 (controls) received adjuvant plus saline solution only. All groups were challenged with 60,000 infective T. colubriformis larvae at 28 days after the last immunization. Significant protection was obtained only when the initial supernatant extract was used to immunize lambs. The proteasome preparation seemed to have immunosuppressive effects through the stimulation of nonspecific IgE production. Significantly lower levels of specific IgE were observed in lambs immunized with the proteasome-enriched fraction, and levels of specific IgG antibodies were increased. We suggest that proteasome fractions of T. colubriformis may serve as useful preparations for the study of mechanisms of IgE production in parasitized sheep.

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Quantification of total sheep IgE concentration using anti-ovine IgE monoclonal antibodies in an enzyme immunoassay

Cited by 33 publications

References 12 publications

Uso De Marcadores Parasitológicos E Imunológicos Na Seleção De Ovelhas Resistentes Às Parasitoses Gastrintestinais

Uso De Marcadores Parasitológicos E Imunológicos Na Seleção De Ovelhas Resistentes Às Parasitoses Gastrintestinais

Immunisation of lambs with drug-abbreviated Haemonchus contortus infections: protection against homologous and heterologous challenge

Immunomodulation of lambs following treatment with a proteasome preparation from infective larvae of Trichostrongylus colubriformis

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