Quantification of uric acid, xanthine and hypoxanthine in human serum by HPLC for pharmacodynamic studies

Cooper, Nancy; Khosravan, Reza; Erdmann, Carol; Fiene, John; Lee, Jean W.

doi:10.1016/j.jchromb.2006.02.060

Cited by 175 publications

(67 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several examples of the use of surrogate matrices for quantitation of exogenous and endogenous molecules have been reported. [28][29][30][31][32][33] We chose rabbit serum because initial observations indicated no interferences with either human or mouse hepcidin detection. Due to the difficulty of synthesizing human and mouse hepcidin, we were not able to use a stablelabeled internal standard that would have been preferred for development of this mass spectrometry-based assay.…”

Section: Resultsmentioning

confidence: 99%

Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry

Murphy¹,

Witcher

Luan

et al. 2007

Blood

149

131

View full text Add to dashboard Cite

The hepatic peptide hormone hepcidin is considered the central regulator of iron metabolism. Characterizing the circulating levels of this peptide is critical to understanding its role in the development of clinically relevant syndromes, such as anemia of inflammation/chronic disease, and may provide insight into potential clinical interventions. While quantitative methods have been published for the determination of urinary hepcidin and serum prohepcidin, no definitive methods have been published for the determination of hepcidin in serum. In this report, we describe a quantitative method for the determination of both human and mouse hepcidin in serum and plasma. The method employs protein precipitation and solid-phase extraction followed by liquid chromatographic separation and tandem mass spectrometry detection. The method has a quantitative range of 0.25 ng/mL to 500 ng/mL serum for mouse hepcidin and 1 ng/mL to 500 ng/mL serum for human hepcidin. The method uses small sample volumes (50 L for mice and 100 L for humans) and 96-well formats for rapid sample processing. The method was used to establish baseline serum and plasma concentrations of hepcidin in normal C57Bl/6 mice and healthy human volunteers. (Blood.

show abstract

Section: Resultsmentioning

confidence: 99%

Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry

Murphy¹,

Witcher

Luan

et al. 2007

Blood

149

131

View full text Add to dashboard Cite

show abstract

“…Stock solutions of uric acid (1 g/L) and the internal standard of 1,[3][4][5][6][7][8][9][10][11][12][13][14][15] N urate (1 g/L) were prepared with boric acid buffer (pH 8.63). All of the stock solutions were kept at 4 C.…”

Section: Preparation Of the Stock Solutionsmentioning

confidence: 99%

“…Although high-performance liquid chromatography with ultraviolet absorbance (HPLC-UV), 7,8 or gas chromatography-tandem mass spectrometry (GC-MS) 9 as a chromatographic method has been developed to measure UA levels, they all have their limitations. The LC techniques have provided the desired sensitivity, but they all lack of good selectivity and high specificity, mainly due to interference in the matrix.…”

Section: Introductionmentioning

confidence: 99%

Determination of Uric Acid in Plasma by LC-MS/MS and Its Application to an Efficacy Evaluation of Recombinant Urate Oxidase

2013

View full text Add to dashboard Cite

Unfortunately, this assay has a narrow linear range, and is not sensitive enough for the analysis of full plasma UA concentration-time profiles after intravenous uricase. Herein, we developed an LC-MS method with excellent sensitivity, high specificity, simple preparation procedures and a short analytical time, which was efficient for analyzing plasma samples at different sampling times after the intravenous infusion of uricase. The efficacy of a new uricase product for injection that was prepared by Escherichia coli has for the first time been investigated in humans. Experimental MaterialsUric acid was purchased from Sigma-Aldrich (St. Louis, MO). . R. ChinaA liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of uric acid in human plasma was developed and validated. Separation was achieved on a C18 column by the mobile phase of 30% water (containing 0.5% formic acid) and 70% methanol. Quantification was done using a multiple reaction monitoring (MRM) mode to monitor the precursor-to-product ion transitions of m/z 169.1 → m/z 141.1 for uric acid and m/z 171 → m/z 143 for 1,3-15 N uric acid (IS) at a positive ionization mode. The calibration curve was established over the range of 0.4096 -100 mg/L, and the correlation coefficient was better than 0.99. The intra-day and inter-day relative standard deviations were less than 5.1%. The accuracy determined at three concentrations ranged between 92.7 and 102.3%. This method was successfully applied to an efficacy study of intravenous recombinant urate oxidase produced by Escherichia coli in a clinical phase I study.

show abstract

“…[1][2] Therefore, simultaneous detection of these two compounds have considerable significance in biochemical and clinical diagnosis. Various methods have been reported to determine the purine degradation products, including high performance liquid chromatography (HPLC), 3 photoion mass spectroscopy, 4 chemiluminescence, 5 enzymatic method, [6][7] capillary electrophoresis (CE) 8 and electrochemistry, 2,9-13 whereas HPLC methods require fastidious sample preparations, prolonged analysis time and expensive materials, enzymatic methods are unstable and very expensive, and CE methods need expensive apparatus, only electrochemical approaches are relatively easy and fast for determination of Xa and HXa.…”

Section: Introductionmentioning

confidence: 99%

The Influencing of Preanodized Inlaying Ultrathin Carbon Paste Electrode on the Oxidation for the Xanthine and Hypoxanthine by the Hydrogen Bond

Qiao

et al. 2015

J Chinese Chemical Soc

View full text Add to dashboard Cite

In this paper, a pre-anodized inlaying ultrathin carbon paste electrode (PAIUCPE) with 316L as a matrix was constructed by a simple and fast electrochemical pretreatment. Using xanthine (Xa) and hypoxanthine (HXa) as the target compounds, the pH effects compositions of buffer solution, the accumulation times, hydrogen bond catalysis, degree of auxiliary electrode reaction on the size of peak currents (I p ) of Xa and HXa was discussed in detail. Also, it was proposed that Xa and HXa were respectively absorbed at the surface of PAIUCPE through hydrogen bonding. The influencing mechanisms of the PAIUCEP on electrochemical oxidation of Xa and HXa were explained in detail. Moreover, the linear relationships for the Xa and HXa were obtained in the range of 6´10 -8 -3´10 -5 mol/L and 2´10 -7 -7´10-5 mol/L, respectively. The detection limits for the Xa and HXa were 1.2´10 -8 mol/L and 5.7´10 -8 mol/L, respectively. Moreover, this proposed method could be applied to determine the Xa and HXa in human urine simultaneously with satisfactory results.

show abstract

Quantification of uric acid, xanthine and hypoxanthine in human serum by HPLC for pharmacodynamic studies

Cited by 175 publications

References 44 publications

Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry

Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry

Determination of Uric Acid in Plasma by LC-MS/MS and Its Application to an Efficacy Evaluation of Recombinant Urate Oxidase

The Influencing of Preanodized Inlaying Ultrathin Carbon Paste Electrode on the Oxidation for the Xanthine and Hypoxanthine by the Hydrogen Bond

Contact Info

Product

Resources

About