Quantification of urinary o,o′-dityrosine, a biomarker for oxidative damage to proteins, by high performance liquid chromatography with triple quadrupole tandem mass spectrometryA comparison with ion-trap tandem mass spectrometry

Orhan, Hilmi; Coolen, Stefan; Meerman, John H.N.

doi:10.1016/j.jchromb.2005.03.043

Cited by 24 publications

(20 citation statements)

References 39 publications

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“…The levels of the measured concentrations of the markers correspond to ranges identified in previously published data, which were measured on the different groups of healthy, non-pregnant subjects (11,12,16,25,26). The concentrations of the markers vary between studies.…”

Section: Discussionsupporting

confidence: 68%

“…The concentrations of the markers vary between studies. These studies in healthy non-pregnant women reported that the urinary HEL levels were between 1.58±0.23 and 2.3±1.2 µmol/mol Cr (11,12,16), PRL levels were 21.6±10.6 µmol/mol Cr (11), DiY levels were between 8.8±0.6 and 10.1±0.4 µmol/mol Cr (16,25) and NY levels were between 0.46±0.49 and 1.4±0.4 µmol/mol Cr (16,26).…”

Section: Discussionmentioning

confidence: 98%

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Lipid-lysine adducts and modified tyrosines as markers of oxidative stress in the second trimester of pregnancy and their association with infant characteristics

Rejc

Kato

Karas-Kuzelicki

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. Pregnancy is a physiological state accompanied by excessive levels of oxidative stress (OS), due to the increased demand and utilisation of oxygen. There is increasing evidence that maternally augmented OS exerts an adverse effect on pregnancy outcome. The aim of the present prospective study was to determine the association between the urinary concentration of relatively novel OS markers measured in the second trimester of pregnancy and the infant characteristics at birth. The maternal levels of urinary hexanoyl-lysine (HEL), propanoyl-lysine (PRL), dityrosine (DiY) and 3-nitrotyrosine (NY) were evaluated in generally healthy pregnant subjects to determine their association with birth weight, gestation at delivery and Apgar score. The observed levels of the markers were in agreement with those measured in healthy non-pregnant subjects in a previous study. A positive correlation was detected between HEL and PRL, as well as between HEL and DiY. Although the absence of a correlation between NY and the other markers has been previously noted in a non-pregnant population, a positive correlation in the pair PRL-NY (r=0.367; P<0.001) was observed in the present study. Maternal cigarette smoking was associated with increased urinary PRL levels (P=0.034). The most notable observation in the present study was that high levels of PRL and NY were associated with low Apgar scores at 1 and 5 min after birth (OR, 1.098 and 2.084 for PRL and NY, respectively; P<0.05). However, poor predictive accuracy was shown. For NY, the following results were obtained: Area under the curve (AUC), 0.818; sensitivity, 100%; specificity, 57%; positive predictive value (PPV), 11.54%; and negative predictive value (NPV), 100%.For PLR the values were as follows: AUC, 0.802; sensitivity, 100%; specificity, 62.6%; PPV, 13.05%; and NPV, 100%. DiY was negatively associated with preterm birth risk (OR=0.703; P=0.028). In conclusion, the results of the present study indicated the presence of OS in the second trimester of pregnancy, which was detected with damage to lipids and proteins and associated with an adverse Apgar score; however, the selected urinary markers exhibited poor positive predictive efficacy.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 98%

Lipid-lysine adducts and modified tyrosines as markers of oxidative stress in the second trimester of pregnancy and their association with infant characteristics

Rejc

Kato

Karas-Kuzelicki

et al. 2016

Experimental and Therapeutic Medicine

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“…These include HPLC with either ultraviolet or fluorescence detection, GC-MS analysis after derivatization, and LC-MS/MS analysis with atmospheric pressure chemical ionization (APCI) or electrospray ionization (139,140 ). Unfortunately, HPLC with ultraviolet or fluorescence detection does not have sufficient specificity for the analysis of urine or tissue extracts because several other protein modifications (e.g., the conversion of tryptophan to N-formylkynurenine and the conjugation of HNE with Lys residues) also yield products that exhibit fluorescence characteristics similar to those of di-Tyr.…”

Section: Clinical Chemistrymentioning

confidence: 99%

“…LC-MS-based methods without derivatization may offer a solution to these problems. Recently, an isotope-dilution reversed-phase LC-APCI-MS/MS method and a triple-quadrupole LC-APCI-MS/MS method have been developed for the quantitative determination of di-Tyr in human urine (140,141 ). Triple-quadrupole LC-APCI-MS/MS, in particular, is a simple, rapid, sensitive, and reliable method that has a detection limit that is 2.5-fold lower than that of isotopedilution reversed-phase LC-APCI-MS/MS.…”

Section: Clinical Chemistrymentioning

confidence: 99%

“…Furthermore, it offers a great advantage over other described methods, as it does not require any pretreatment other than centrifClinical Chemistry 52, No. 4,2006 ugation of the urine sample and addition of the labeled internal standard before injection (140 ). Actually, reliable quantification of di-Tyr, as well as of NO 2 -Tyr, in urine and plasma is attainable only with the MS/MS technology (113,140 ) …”

Section: Clinical Chemistrymentioning

confidence: 99%

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Biomarkers of Oxidative Damage in Human Disease

et al. 2006

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Oxidative/nitrosative stress, a pervasive condition of increased amounts of reactive oxygen/nitrogen species, is now recognized to be a prominent feature of many acute and chronic diseases and even of the normal aging process. However, definitive evidence for this association has often been lacking because of recognized shortcomings with biomarkers and/or methods available to assess oxidative stress status in humans. Emphasis is now being placed on biomarkers of oxidative stress, which are objectively measured and evaluated as indicators of normal biological processes, pathogenic processes, or pharmacologic responses to therapeutic intervention. To be a predictor of disease, a biomarker must be validated. Validation criteria include intrinsic qualities such as specificity, sensitivity, degree of inter-and intraindividual variability, and knowledge of the confounding and modifying factors. In addition, characteristics of the sampling and analytical procedures are of relevance, including constraints and noninvasiveness of sampling, stability of potential biomarkers, and the simplicity, sensitivity, specificity, and speed of the analytical method. Here we discuss some of the more commonly used biomarkers of oxidative/nitrosative damage and include selected examples of human studies.

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Mass Spectrometry in the Analysis of DNA, Protein, Peptide, and Lipid Biomarkers of Oxidative Stress

Gelhaus

Blair

2011

Mass Spectrometry in Drug Metabolism and Disposition

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Quantification of urinary o,o′-dityrosine, a biomarker for oxidative damage to proteins, by high performance liquid chromatography with triple quadrupole tandem mass spectrometryA comparison with ion-trap tandem mass spectrometry

Cited by 24 publications

References 39 publications

Lipid-lysine adducts and modified tyrosines as markers of oxidative stress in the second trimester of pregnancy and their association with infant characteristics

Lipid-lysine adducts and modified tyrosines as markers of oxidative stress in the second trimester of pregnancy and their association with infant characteristics

Biomarkers of Oxidative Damage in Human Disease

Mass Spectrometry in the Analysis of DNA, Protein, Peptide, and Lipid Biomarkers of Oxidative Stress

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