Quantification of viable helminth eggs in samples of sewage sludge

Rocha, Maria Carolina Vieira da; Barés, Monica Eboly; Braga, Maria Cristina Borba

doi:10.1016/j.watres.2016.07.039

Cited by 31 publications

(29 citation statements)

References 70 publications

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“…Nonetheless, in complex matrices such as sewage, there is considerable uncertainty regarding both the concentrations detected and the ratios between noninfectious and infectious units. Quantities detected by molecular methods are greater than those obtained by culture methods (Johnson et al 1995;Kong et al 2002;Girones et al 2010;Hamza et al 2011;Rocha et al 2016). Nucleic acid amplification techniques based on membrane integrity have been developed for preferential detection of viable bacteria, such as ethidium monoazide PCR (EMA-PCR) and propidium monoazide PCR (PMA-PCR).…”

Section: Pathogens In Raw Sewagementioning

confidence: 99%

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Pathogens, faecal indicators and human-specific microbial source-tracking markers in sewage

et al. 2019

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Summary The objective of this review is to assess the current state of knowledge of pathogens, general faecal indicators and human‐specific microbial source tracking markers in sewage. Most of the microbes present in sewage are from the microbiota of the human gut, including pathogens. Bacteria and viruses are the most abundant groups of microbes in the human gut microbiota. Most reports on this topic show that raw sewage microbiological profiles reflect the human gut microbiota. Human and animal faeces share many commensal microbes as well as pathogens. Faecal‐orally transmitted pathogens constitute a serious public health problem that can be minimized through sanitation. Assessing both the sanitation processes and the contribution of sewage to the faecal contamination of water bodies requires knowledge of the content of pathogens in sewage, microbes indicating general faecal contamination and microbes that are only present in human faecal remains, which are known as the human‐specific microbial source‐tracking (MST) markers. Detection of pathogens would be the ideal option for managing sanitation and determining the microbiological quality of waters contaminated by sewage; but at present, this is neither practical nor feasible in routine testing. Traditionally, faecal indicator bacteria have been used as surrogate indicators of general faecal residues. However, in many water management circumstances, it becomes necessary to detect both the origin of faecal contamination, for which MST is paramount, and live micro‐organisms, for which molecular methods are not suitable. The presence and concentrations of pathogens, general faecal indicators and human‐specific MST markers most frequently reported in different areas of the world are summarized in this review.

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Section: Pathogens In Raw Sewagementioning

confidence: 99%

“…; Rocha et al . ). Nucleic acid amplification techniques based on membrane integrity have been developed for preferential detection of viable bacteria, such as ethidium monoazide PCR (EMA‐PCR) and propidium monoazide PCR (PMA‐PCR).…”

Section: Introductionmentioning

confidence: 97%

Pathogens, faecal indicators and human-specific microbial source-tracking markers in sewage

et al. 2019

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“…Some of them are pathogens for humans and animals and are responsible for various diseases (Chaoua et al, 2017). Sludge frequently contains helminth eggs (Ascaris, Trichuris, Toxocara) (Da Rocha et al, 2016), which are among the most resistant organisms to sludge treatment. Their survival has already been observed for several years after the biosolid to soil application (Bloem et al, 2017).…”

Section: Pathogenic Organismsmentioning

confidence: 99%

Sanitary and environmental aspects of sewage sludge management

Rorat

Courtois

Vandenbulcke

et al. 2019

Industrial and Municipal Sludge

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“…However, by its origin -from the sewage treatment -the sludge concentrates many elements, which can pose risks to the environment and public health, such as heavy metals, pharmaceutical contaminants, polyaromatic hydrocarbons and pathogens (Andreoli et al, 2008, Cheng et al, 2012, Jones, Gardner, & Ellor, 2014, Cieslik, Namiesnik, & Konieczka, 2015, Gyawali, Sidhu, Ahmed, Jagals, & Toze, 2015, Rocha, Barés, & Braga, 2016.There is little information on the number and survival of pathogens in biosolids because of difficulties with detection methods, especially for viruses and protozoa (Sidhu & Toze, 2009).…”

Section: Introductionmentioning

confidence: 99%

<b>Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge

Medeiros

Daniel

2018

Acta Sci. Technol.

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ABSTRACT. For the use of sewage sludge, it is extremely important to consider the microbiological aspect of this byproduct that poses direct and indirect risks to public health as for its inadequate handling and use. This study aimed to quantify Giardia spp. cysts and Cryptosporidium spp. oocysts and infer their viability in sludge samples from two sewage treatment plants. The method used consisted of three successive centrifugations followed by immunofluorescence assay and staining with propidium iodide. Samples were 100% (14/14) positive for cysts and 57.1% (8/14) for oocysts, with higher concentrations and mean viability above 75%. The change in the treatment of activated sludge, from extended aeration to conventional system, caused no difference in the concentration and viability of (oo)cysts.

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Quantification of viable helminth eggs in samples of sewage sludge

Cited by 31 publications

References 70 publications

Pathogens, faecal indicators and human-specific microbial source-tracking markers in sewage

Pathogens, faecal indicators and human-specific microbial source-tracking markers in sewage

Sanitary and environmental aspects of sewage sludge management

<b>Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge

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