Quantification of water-soluble vitamins in milk-based infant formulae using biosensor-based assays

Yan-ling, Gao; Guo, Fei; Gokavi, Sumangala; Chow, Andrew; Qing-hai, Sheng; Guo, Mingruo

doi:10.1016/j.foodchem.2008.03.007

Cited by 57 publications

(40 citation statements)

References 26 publications

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“…Subnormal vitamin B 12 levels have been linked to reversible dementia [30], while vitamin B 12 deficiency results in pernicious anaemia and is also manifested by neurological changes which could culminate in brain damage [6,16]. Deficiency had also been associated with cerebellar ataxia [26], triggering seizures in epileptics [29] and reversible involuntary movements in adults [8].…”

Section: Introductionmentioning

confidence: 99%

“…However, since activity may be lost during processing and storage, quantification of vitamin B 12 is needed to ensure quality control, to satisfy government regulatory requirements, including nutrition labelling [3,12].…”

Section: Introductionmentioning

confidence: 99%

“…Vitamin B 12 assay techniques include microbiological assays, radioisotope dilution assay, electrothermal atomic absorption spectrometry, capillary electrophoresis, chemiluminescence techniques, biomolecular interaction analysis (BIA) and a combination of fluorescence resonance energy transfer (FRET) and flow-injection analysis (FIA) [1,6,12,22,33]. The microbiological assay is still the official method, but is very laborious [1].…”

Section: Introductionmentioning

confidence: 99%

“…Pour lutter contre les carences en vitamine B 12 parmi certaines populations d'Afrique du Sud, des aliments fonctionnels enrichis ont été développés. Cependant, les méthodes actuelles utilisées pour analyser précisément le niveau de vitamine B 12 dans les aliments, qui sont importantes à des fins de contrôle-qualité et de législation, sont coûteuses en temps.…”

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“…Les échantillons étaient extraits par mélange à du tampon KCN, pH 4,5 et autoclavage à 121°C pendant 25 min, refroidissement et centrifugation. Le surnageant résultant était filtré puis analysé par HPLC en phase inverse en utilisant un gradient méthanol-eau, qui permettait une bonne résolution du pic de vitamine B 12 . La méthode montrait une bonne linéarité, avec un coefficient de régression R > 0,998.…”

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A rapid HPLC method for the extraction and quantification of vitamin B₁₂in dairy products and cultures ofPropionibacterium freudenreichii

Wyk

Britz

2010

Dairy Sci. Technol.

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-To overcome nutritional vitamin B 12 deficiencies in certain populations in Southern Africa, fortified functional foods have been developed. However, current microbiological methods used to accurately determine vitamin B 12 levels in foodstuffs, important for quality control and regulatory purposes, are time consuming. This study describes an extraction and detection method for vitamin B 12 in dairy products and growth media cultured with Propionibacterium freudenreichii. Samples were extracted by mixing in KCN buffer (pH 4.5), autoclaving at 121°C for 25 min, cooling and centrifugation. The resultant supernatant was syringe-filtered prior to reversed-phase HPLC analysis using a methanol-water gradient that was effective in resolving the B 12 peak. The method offered excellent linearity with a regression coefficient R > 0.998. The limit of quantification was 0.005 μg·mL −1 sample. For samples with vitamin B 12 concentrations well within the linear range of the assay, good repeatability was demonstrated for the same sample with mean concentrations of 2.62 ± 0.02 and 2.61 ± 0.02 μg·mL −1 detected on day 1 and day 2, respectively. Recovery values ranged from 98.6% to 103.2%, indicating that the extraction method ensured complete dissolution of vitamin B 12 from the matrices under study. Sensitivity was enhanced by sample concentration and purification using a series of solid phase extraction steps which resulted in improved peak resolution and removal of interfering peaks. The method was validated by comparison of the HPLC results of the same sample with those obtained using traditional microbiological methods. The method is a rapid alternative to the more time-consuming microbiological assay. Résumé -Une méthode HPLC rapide pour extraire et quantifier la vitamine B 12 dans les produits laitiers et les cultures de Propionibacterium freudenreichii. Pour lutter contre les carences en vitamine B 12 parmi certaines populations d'Afrique du Sud, des aliments fonctionnels enrichis ont été développés. Cependant, les méthodes actuelles utilisées pour analyser précisément le niveau de vitamine B 12 dans les aliments, qui sont importantes à des fins de contrôle-qualité et de législation, sont coûteuses en temps. Cet article décrit une méthode d'extraction et de détection de la vitamine B 12 dans les produits laitiers et les cultures de Propionibacterium freudenreichii. Les échantillons étaient extraits par mélange à du tampon KCN, pH 4,5 et autoclavage à 121°C pendant 25 min, refroidissement et centrifugation. Le surnageant résultant était filtré puis analysé par HPLC en phase inverse en utilisant un gradient méthanol-eau, qui permettait une bonne résolution du pic de vitamine B 12 . La méthode montrait une bonne linéarité, avec un coefficient de régression R > 0,998. La limite de quantification était de 0,005 μg·mL −1 d'échantillon. Pour des échantillons contenant des concentrations dans la moyenne de la gamme de linéarité, la répétabilité a été établie, avec des valeurs obtenues pour le même échantillon à deux jo...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

unclassified

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A rapid HPLC method for the extraction and quantification of vitamin B₁₂in dairy products and cultures ofPropionibacterium freudenreichii

Wyk

Britz

2010

Dairy Sci. Technol.

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Recent Advances of Biosensors in Food Detection Including Genetically Modified Organisms in Food

Varzakas

Nikoleli

Nikolelis

2015

Advanced Bioelectronic Materials

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Immunology‐based Biosensors

Varzakas

Nikoleli

Nikolelis

2017

Advances in Food Diagnostics

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Quantification of water-soluble vitamins in milk-based infant formulae using biosensor-based assays

Cited by 57 publications

References 26 publications

A rapid HPLC method for the extraction and quantification of vitamin B₁₂in dairy products and cultures ofPropionibacterium freudenreichii

A rapid HPLC method for the extraction and quantification of vitamin B₁₂in dairy products and cultures ofPropionibacterium freudenreichii

Recent Advances of Biosensors in Food Detection Including Genetically Modified Organisms in Food

Immunology‐based Biosensors

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Quantification of water-soluble vitamins in milk-based infant formulae using biosensor-based assays

Cited by 57 publications

References 26 publications

A rapid HPLC method for the extraction and quantification of vitamin B12in dairy products and cultures ofPropionibacterium freudenreichii

A rapid HPLC method for the extraction and quantification of vitamin B12in dairy products and cultures ofPropionibacterium freudenreichii

Recent Advances of Biosensors in Food Detection Including Genetically Modified Organisms in Food

Immunology‐based Biosensors

Contact Info

Product

Resources

About

A rapid HPLC method for the extraction and quantification of vitamin B₁₂in dairy products and cultures ofPropionibacterium freudenreichii

A rapid HPLC method for the extraction and quantification of vitamin B₁₂in dairy products and cultures ofPropionibacterium freudenreichii