Quantification of West Nile Virus by Plaque-Forming Assay

Neupane, Biswas; Bai, Fengwei

doi:10.1007/978-1-0716-2760-0_2

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Remdesivir-loaded Nanocomposites Inhibit Sars-cov-2 Infectio...1

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2024

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Cited by 3 publications

(1 citation statement)

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“…Plaque assays have been considered one of the most precise methods for the direct quantication of viruses through counting the isolated plaques in cell culture. [48][49][50] The plaque assay was performed following the guidelines of previous studies, to estimate the viral titer from the supernatants that were collected aer 24 hours of drug treatment in Vero E6 cells pre-infected with SARS-CoV-2. 49,51 The virus titer from different wells was calculated in PFU mL −1 , and differences in the viral titer were compared to the untreated virus control (Fig.…”

Section: Remdesivir-loaded Nanocomposites Inhibit Sars-cov-2 Infectio...mentioning

confidence: 99%

A novel nanocomposite drug delivery system for SARS-CoV-2 infections

Chintapula,

Karim,

Iyer

et al. 2024

Nanoscale Adv.

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Section: Remdesivir-loaded Nanocomposites Inhibit Sars-cov-2 Infectio...mentioning

confidence: 99%

A novel nanocomposite drug delivery system for SARS-CoV-2 infections

Chintapula,

Karim,

Iyer

et al. 2024

Nanoscale Adv.

Self Cite

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Investigating TIP30-Mediated regulation of mTORC1 signaling as a therapeutic strategy for coxsackievirus B3-Induced viral myocarditis

Liu,

Hou,

et al. 2024

Virology

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Improved Zika virus plaque assay using Vero/TMPRSS2 cell line

Oluwasemowo,

Graham,

Murugesh

et al. 2024

Preprint

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Plaque assay is the gold standard for the quantification of viable cytopathic viruses like Zika virus (ZIKV). Some strains of ZIKV produce plaques that are very difficult to accurately visualize and count on the commonly used Vero cell line. From data generated in our lab, we became curious if Vero/TMPRSS2 cells may be a better alternative, therefore we compared the plaque forming units (PFU) of two strains of ZIKV on Vero/TMPRSS2 cells to those produced by Vero cells. We also compared the virus stock titer generated on Vero/TMPRSS2 cells to that generated by the Vero cell line. Although Vero cells generated higher quantity of ZIKV stocks, Vero/TMPRSS2 cells produced plaques with significantly improved morphology and visibility and may therefore be a better alternative to use for performing plaque assays for strains of ZIKV that are more difficult to titer on regular Vero cells.

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Quantification of West Nile Virus by Plaque-Forming Assay

Cited by 3 publications

References 5 publications

A novel nanocomposite drug delivery system for SARS-CoV-2 infections

A novel nanocomposite drug delivery system for SARS-CoV-2 infections

Investigating TIP30-Mediated regulation of mTORC1 signaling as a therapeutic strategy for coxsackievirus B3-Induced viral myocarditis

Improved Zika virus plaque assay using Vero/TMPRSS2 cell line

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