Quantification of α-hydroxy ceramides in mice serum by LC-MS/MS: Application to sepsis study

Chao, Yufan; Chen, Xiaoqing; Shi, Xuan; Li, Na; Gao, Songyan; Yang, Jing‐Zhi; Dong, Xin

doi:10.1016/j.jchromb.2023.123764

Cited by 1 publication

(1 citation statement)

References 37 publications

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“…LC efficiently purifies sphingolipid metabolites, isolating them from complex mixtures while reducing isomer interference and thereby improving the accuracy and sensitivity of subsequent mass spectrometry analysis. By utilizing this combination, one can accurately identify various sphingolipids such as ceramide, hexosylceramides, lactosylceramide, sphingomyelin, sphingolipid phosphorylation products, and other complex sphingolipids [ 40 , 41 , 42 , 43 , 44 ]. LC can adopt two separation modes: normal-phase chromatography and reverse-phase chromatography.…”

Section: Mass Spectrometry Detected Sphingolipid Biomarkers For Carci...mentioning

confidence: 99%

Mass Spectrometry Detects Sphingolipid Metabolites for Discovery of New Strategy for Cancer Therapy from the Aspect of Programmed Cell Death

Shi

Tang

et al. 2023

Metabolites

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Sphingolipids, a type of bioactive lipid, play crucial roles within cells, serving as integral components of membranes and exhibiting strong signaling properties that have potential therapeutic implications in anti-cancer treatments. However, due to the diverse group of lipids and intricate mechanisms, sphingolipids still face challenges in enhancing the efficacy of different therapy approaches. In recent decades, mass spectrometry has made significant advancements in uncovering sphingolipid biomarkers and elucidating their impact on cancer development, progression, and resistance. Primary sphingolipids, such as ceramide and sphingosine-1-phosphate, exhibit contrasting roles in regulating cancer cell death and survival. The evasion of cell death is a characteristic hallmark of cancer cells, leading to treatment failure and a poor prognosis. The escape initiates with long-established apoptosis and extends to other programmed cell death (PCD) forms when patients experience chemotherapy, radiotherapy, and/or immunotherapy. Gradually, supportive evidence has uncovered the fundamental molecular mechanisms underlying various forms of PCD leading to the development of innovative molecular, genetic, and pharmacological tools that specifically target sphingolipid signaling nodes. In this study, we provide a comprehensive overview of the sphingolipid biomarkers revealed through mass spectrometry in recent decades, as well as an in-depth analysis of the six main forms of PCD (apoptosis, autophagy, pyroptosis, necroptosis, ferroptosis, and cuproptosis) in aspects of tumorigenesis, metastasis, and tumor response to treatments. We review the corresponding small-molecule compounds associated with these processes and their potential implications in cancer therapy.

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Section: Mass Spectrometry Detected Sphingolipid Biomarkers For Carci...mentioning

confidence: 99%

Mass Spectrometry Detects Sphingolipid Metabolites for Discovery of New Strategy for Cancer Therapy from the Aspect of Programmed Cell Death

Shi

Tang

et al. 2023

Metabolites

View full text Add to dashboard Cite

show abstract

Quantification of α-hydroxy ceramides in mice serum by LC-MS/MS: Application to sepsis study

Cited by 1 publication

References 37 publications

Mass Spectrometry Detects Sphingolipid Metabolites for Discovery of New Strategy for Cancer Therapy from the Aspect of Programmed Cell Death

Mass Spectrometry Detects Sphingolipid Metabolites for Discovery of New Strategy for Cancer Therapy from the Aspect of Programmed Cell Death

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