Quantification of δ-, γ- and α-Tocopherol in Tomatoes Using an Improved Liquid-Dispersive Solid-Phase Extraction Combined with Ultrahigh Pressure Liquid Chromatography

Figueira, José A.; Câmara, José S.

doi:10.1007/s12161-017-0799-0

Cited by 8 publications

(6 citation statements)

References 72 publications

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“…In previous works, a high concentration in different bioactive compounds, particularly phenolic compounds, has been observed in the fruits analysed in this work [ 5 , 9 , 10 , 15 , 25 ]. This observation led us to investigate the bioactive potential of the juice and peels of lemon and tangerine, pulp and seeds of pitanga, and the whole fruit of tomato and uva-da-serra.…”

Section: Resultsmentioning

confidence: 62%

“…As shown in Figure 2 , the solvent mixtures ACN:MeOH (4:1, v / v ), MeOH:FA (19:1, v/v ) and ACN retrieve the best results. ACN:MeOH (4:1, v/v ) was the selected condition as it was previously used with success in the extraction of hydrophilic and lipophilic bioactive compounds (carotenoids or tocopherols) [ 5 , 25 ].…”

Section: Resultsmentioning

confidence: 99%

“…In this sense, Madeira Island has very challenging climate conditions, with very hot and humid conditions all over the year, high thermic variations and pronounced slopes which correlate with the high bioactive potential and complex volatile compositions exhibited by fruits growing in Madeira Island in comparison with same varieties from other geographical regions. Previously, we have shown that tomato ( Solanum lycopersicum L.) from Gordal variety grown in Madeira Island presented higher tocopherols content when compared with other varieties commonly consumed like Campari, Roma and cherry [ 5 ]. In addition, recently, Detopoulou et al [ 6 ] underlined the relevance of tocopherols, as vitamin E, on the proper functioning of the immune system, acting as an antioxidant in the context of the importance of phytochemicals of the Mediterranean diet against COVID-19 effects.…”

Section: Introductionmentioning

confidence: 99%

“…In this context, this study aimed to evaluate the health-promoting proprieties of fruits of regular consumption grown in Madeira Island, including the total phenolic and flavonoid contents (TPC and TFC, respectively), antioxidant capacity (ABTS and DPPH assays) and its ability to inhibit digestive enzymes linked to diabetes (α-amylase, α- and β-glucosidase) and hypertension (angiotensin-converting enzyme). The fruits were selected according to its high bioactive potential, previously reported, namely tomato (gordal variety) [ 5 , 25 ], lemon (eureka variety) [ 10 ], tangerine (setubalense variety) [ 9 ], uva-da-serra [ 15 ], and pitanga. Liquid-liquid-based ultrasound-assisted extraction (LLUSAE), an efficient extraction procedure for vegetable matrices, previously optimized in our lab [ 25 ], was used to obtain the extracts.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Health-Promoting Properties of Selected Fruits

et al. 2021

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In this study, the health-promoting benefits of different fruits grown in Madeira Island, namely lemon (Citrus limon var. eureka), tangerine (Citrus reticulata var. setubalense), pitanga (Eugenia uniflora var. red), tomato (Solanum lycopersicum var. gordal) and uva-da-serra, an endemic blueberry (Vaccinium padifolium Sm.), were investigated. The phenolic composition (total phenolics and total flavonoids content) and antioxidant capacity (assessed through ABTS and DPPH assays) were measured revealing a high phenolic potential for all fruits, except tomato, while uva-da-serra is particularly rich in flavonoids. In relation to the antioxidant capacity, the highest values were obtained for pitanga and uva-da-serra extracts. The bioactive potential was also assessed through the ability of the extracts to inhibit digestive enzymes linked to diabetes (α-amylase, α- and β-glucosidases) and hypertension (angiotensin-converting enzyme, ACE). The results obtained point to a very high bioactive potential with the selected samples exhibiting very important ACE anti-enzymatic capacities. A statistical analysis of the obtained data reveals a very strong correlation between ABTS and TPC, and a strong contribution of the fruit polyphenols for enzyme inhibition, and thus, presenting high antihypertensive and antidiabetic capacities. Overall, the results obtained clearly show a high bioactive potential of the selected fruits that should be further studied, in terms of specific phenolic composition. Moreover, these results strongly support the valorisation of pitanga seeds usually discarded as a waste, and uva-da-serra, an endemic and wild bush, as potential bioresources of bioactive compounds with impact in human diet.

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Section: Resultsmentioning

confidence: 62%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Health-Promoting Properties of Selected Fruits

et al. 2021

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“…To quantify α‐, γ‐, and δ‐Ts in tomatoes, Figueira et al. extracted the analytes by using methanol–acetonitrile and purified with a composite of PSA‐C 18 ‐MgSO 4 .…”

Section: Introductionmentioning

confidence: 99%

A convenient ultrasound‐assisted saponification for the simultaneous determination of vitamin E isomers in vegetable oil by HPLC with fluorescence detection

Yang

Yin

et al. 2018

J of Separation Science

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An efficient ultrasound-assisted saponification was developed for simultaneous determination of vitamin E isomers in vegetable oil by high-performance liquid chromatography with fluorescence detection. The samples were saponified ultrasonically with potassium hydroxide solution for only 7 min, then the analytes were extracted with ether. Vitamin E isomers were separated on a C column at 25°C with a mobile phase of methanol/acetonitrile (81:19, v/v) at a flow rate of 0.8 mL/min. Fluorescence detection was operated at 290 nm of excitation wavelength and 327 nm of emission wavelength. Under the optimized conditions, good linearities over the range of 0.001-8.00 μg/mL (r > 0.999) were obtained. Mean recoveries of the method were 88.0-106%, with intra- and interday RSDs less than 11.8 and 12.8%, respectively. The detection limits and quantification limits of the method were 0.30-1.8 and 1.0-6.1 μg/kg, respectively. The recoveries of this method were much higher than that of the quick, easy, cheap, effective, rugged, and safe method and direct dilution method, but were similar to those of hot saponification. This proposed method provides reliable, simple, and rapid quantification of vitamin E isomers in vegetable oils. Five kinds of vegetable oils were analyzed, the quantification results were within the ranges reported by other authors.

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2019

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Quantification of δ-, γ- and α-Tocopherol in Tomatoes Using an Improved Liquid-Dispersive Solid-Phase Extraction Combined with Ultrahigh Pressure Liquid Chromatography

Cited by 8 publications

References 72 publications

Evaluation of the Health-Promoting Properties of Selected Fruits

Evaluation of the Health-Promoting Properties of Selected Fruits

A convenient ultrasound‐assisted saponification for the simultaneous determination of vitamin E isomers in vegetable oil by HPLC with fluorescence detection

Analysis of Frying

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