Quantifizierung der Zellaufnahme metallierter Peptide und Peptidnucleinsäuren durch Atomabsorptionsspektroskopie

Kirin, Srećko I.; Ingo, Ott; Gust, Ronald; Mier, Walter; Weyhermüller, Thomas; Metzler‐Nolte, Nils

doi:10.1002/ange.200703994

Cited by 18 publications

(17 citation statements)

References 51 publications

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“…The QCM-D technique can also determine the penetration depth for a particular frequency wave, holding an inverse relationship with the overtone number. 56 This implies that the 15 higher harmonics can probe more closely to the sensor surface and vice versa. Thus, a comprehensive understanding including the kinetics of PNA-membrane interaction in a three dimensional membrane structure can be readily obtained by measurements at several different harmonics.…”

Section: Resultsmentioning

confidence: 99%

“…Ultrapure water with an initial resistivity of 18.2 MΩ·cm was used for all experiments. Phosphate buffered saline (20 mM KH 2 PO 4 and K 2 HPO 4 , pH 15 6.9±0.1) with 100 mM sodium chloride (high-salt PBS) and 10 mM sodium chloride (low-salt PBS) were prepared in water.…”

Section: Methodsmentioning

confidence: 99%

“…6,11,15,17,[21][22] have shown promising improvements in cellular bioavailability, there is still a need to improve our understanding of cellular uptake for different 50 delivery vectors and to provide better correlations of their in vivo structure-activity relationships.…”

Section: +mentioning

confidence: 99%

“…13 Although much attention has been focussed on the preparation of such constructs [37][38][39][40][41] and only recently have papers appeared which report the applications of non-radioactive metal 75 bioconjugates in living cells. [14][15][42][43][44] It is therefore important to understand the mechanism of uptake of these nucleotide analogues.…”

Section: +mentioning

confidence: 99%

“…These include co-delivery with partial cDNA oligonucleotides, cationic lipids and lipophilic triphenylphosphonium (TPP) cations, and a few examples of chemical modifications of PNA oligomers with metal complexes. 6,8,[11][12][13][14][15] However, to date, the most promising 30 approach has been the conjugation of PNAs with cell penetrating peptides (CPP). 6,8,12 Although the mechanism of translocation of CPPs is not fully understood, their ability to successfully transport cargo (e.g., PNAs) into the cell makes them effective delivery agents.…”

Section: Introductionmentioning

confidence: 99%

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Specific uptake and interactions of peptide nucleic acid derivatives with biomimetic membranes

et al. 2012

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There is a growing interest in understanding the uptake mechanism of metal-containing peptide nucleic acid (PNA) bioconjugates into living cells. In this study, quartz crystal microbalance with dissipation monitoring (QCM-D) has been used to explore the membrane specific uptake and interactions of PNA/peptide/Ru(II) conjugates. For all lipid compositions, the unmodified PNA oligomer and its Ru(II) conjugate were found to traverse freely across the membrane in a trans-membrane manner, causing no significant changes in the membrane structure. The nuclear localised signal peptide (NLS) conjugated sequences showed membrane specific activities. In model mammalian and bacterial-mimetic membranes, rapid trans-membrane insertion was observed followed by a concentration dependent disruption and irreversible structural changes to the membrane system. The variations in the magnitude of the structural changes and in their tendency to facilitate disruption are ascribed to hydrophobicity, the cationic charge introduced on modification of the original PNA backbone as well as the physical state of the model membrane used. There is a growing interest in understanding the uptake mechanism of metal-containing peptide nucleic acid (PNA) bioconjugates into living cells. In this study, Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) has been used to explore the membrane specific uptake and interactions of PNA/peptide/Ru(II) conjugates. For all lipid compositions, the unmodified PNA oligomer and its Ru(II) conjugate were found to traverse freely across the membrane in a trans-membrane manner, causing no significant changes in 10 membrane structure. The Nuclear Localised Signal Peptide (NLS) conjugated sequences showed membrane specific activities. In model mammalian and bacterial-mimetic membranes, rapid trans-membrane insertion was observed followed by a concentration dependent disruption and irreversible structural changes to the membrane system. The variations in the magnitude of the structural changes and in their tendency to facilitate disruption are ascribed to hydrophobicity, the cationic charge introduced on modification of the original PNA 15 backbone as well as the physical state of the model membrane used.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: +mentioning

confidence: 99%

Section: +mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Specific uptake and interactions of peptide nucleic acid derivatives with biomimetic membranes

et al. 2012

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Markierungsfreie Visualisierung von löslichen Metallcarbonylkomplexen in lebenden Zellen mithilfe von Raman‐Mikrospektroskopie

et al. 2010

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A Ratiometric Fluorescent Probe for Cisplatin: Investigating the Intracellular Reduction of Platinum(IV) Prodrug Complexes

Ong

Lim

et al. 2018

Angewandte Chemie

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The PtIV prodrug strategy has emerged as an excellent alternative to tackle the problems associated with conventional PtII drug therapy. However, there is a lack of tools to study how this new class of PtIV drugs are processed at the cellular level. Herein, we report the first ratiometric probe for cisplatin detection and use it to investigate PtIV anticancer complexes in biological systems. The probe was able to distinguish between cisplatin and its PtIV derivatives, allowing us to probe the intracellular reduction of PtIV prodrug complexes. The correlation between the amount of active PtII species available after intracellular reduction of PtIV complexes and their cytotoxicity and the role glutathione plays in the reduction of PtIV complexes were investigated.

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Quantifizierung der Zellaufnahme metallierter Peptide und Peptidnucleinsäuren durch Atomabsorptionsspektroskopie

Cited by 18 publications

References 51 publications

Specific uptake and interactions of peptide nucleic acid derivatives with biomimetic membranes

Specific uptake and interactions of peptide nucleic acid derivatives with biomimetic membranes

Markierungsfreie Visualisierung von löslichen Metallcarbonylkomplexen in lebenden Zellen mithilfe von Raman‐Mikrospektroskopie

A Ratiometric Fluorescent Probe for Cisplatin: Investigating the Intracellular Reduction of Platinum(IV) Prodrug Complexes

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