2021
DOI: 10.1186/s12896-021-00689-4
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Quantifying 35 transcripts in a single tube: model-based calibration of the GeXP multiplex RT-PCR assay

Abstract: Background Quantitative analysis of differential gene expression is of central importance in molecular life sciences. The Gene eXpression Profiling technology (GeXP) relies on multiplex RT-PCR and subsequent capillary electrophoretic separation of the amplification products and allows to quantify the transcripts of at least 35 genes with a single reaction and one dye. Results We provide a kinetic model of primer binding and PCR product formation as… Show more

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