2024
DOI: 10.1111/1541-4337.70029
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Quantifying allergenic proteins using antibody‐based methods or liquid chromatography–mass spectrometry/mass spectrometry: A review about the influence of food matrix, extraction, and sample preparation

Wei Hu,
Xing Zhang,
Yunpeng Shen
et al.

Abstract: Accurate quantification of allergens in food is crucial for ensuring consumer safety. Pretreatment steps directly affect accuracy and efficiency of allergen quantification. We systematically reviewed the latest advances in pretreatment steps for antibody‐based methods and liquid chromatography–mass spectrometry/mass spectrometry (LC–MS/MS) protein quantification methods in food. For antibody‐based methods, the effects induced by food matrix like decreased allergen solubility, epitope masking, and nonspecific b… Show more

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