Quantifying cell-cycle-dependent chromatin dynamics during interphase by live 3D tracking

Naor, Tal; Nogin, Yevgeni; Nehme, Elias; Ferdman, Boris; Weiss, Lucien E.; Alalouf, Onit; Shechtman, Yoav

doi:10.1016/j.isci.2022.104197

Cited by 4 publications

(3 citation statements)

References 72 publications

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“…We point out that the variations in chromatin architecture and dynamics across different cell phases are highly dependent on the spatial and temporal scales of the measurements. For example, in the timescale of approximately 10 s, single telomeres exhibit different diffusion characteristics in the interphases where chromatin diffusion is more restricted in the S and G2 phases than in the G1 phase 57 . On the other hand, in a shorter timescale of sub-second, telomere diffusion exhibits no significant differences throughout the interphase.…”

Section: Resultsmentioning

confidence: 99%

Probing chromatin condensation dynamics in live cells using interferometric scattering correlation spectroscopy

Hsiao,

Liao,

et al. 2024

Commun Biol

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Chromatin organization and dynamics play important roles in governing the regulation of nuclear processes of biological cells. However, due to the constant diffusive motion of chromatin, examining chromatin nanostructures in living cells has been challenging. In this study, we introduce interferometric scattering correlation spectroscopy (iSCORS) to spatially map nanoscopic chromatin configurations within unlabeled live cell nuclei. This label-free technique captures time-varying linear scattering signals generated by the motion of native chromatin on a millisecond timescale, allowing us to deduce chromatin condensation states. Using iSCORS imaging, we quantitatively examine chromatin dynamics over extended periods, revealing spontaneous fluctuations in chromatin condensation and heterogeneous compaction levels in interphase cells, independent of cell phases. Moreover, we observe changes in iSCORS signals of chromatin upon transcription inhibition, indicating that iSCORS can probe nanoscopic chromatin structures and dynamics associated with transcriptional activities. Our scattering-based optical microscopy, which does not require labeling, serves as a powerful tool for visualizing dynamic chromatin nano-arrangements in live cells. This advancement holds promise for studying chromatin remodeling in various crucial cellular processes, such as stem cell differentiation, mechanotransduction, and DNA repair.

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Section: Resultsmentioning

confidence: 99%

Probing chromatin condensation dynamics in live cells using interferometric scattering correlation spectroscopy

Hsiao,

Liao,

et al. 2024

Commun Biol

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“…The distinct nucleolar structure of proliferating ASCs may therefore be related to the frequent re-assembly of nucleoli after cell division. Nucleolus assembly is driven by liquidliquid phase separation 38,39 , and changes in nucleolar structure might re ect variations in the physical nucleoplasmic environment, as chromatin mobility differs between dividing and growth-arrested cells 40,41 .…”

Section: Discussionmentioning

confidence: 99%

Cytoskeletal remodeling defines nucleolar architecture during adipogenesis

Potolitsyna

Pickering

Germier

et al. 2023

Preprint

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Differentiation of adipose progenitor cells into mature adipocytes entails a dramatic reorganization of the cellular architecture to accommodate lipid storage into cytoplasmic lipid droplets. Lipid droplets occupy most of the adipocyte volume, compressing the nucleus beneath the plasma membrane. How this cellular remodeling affects sub-nuclear structure, including size and number of nucleoli, remains unclear. We describe the morphological remodeling of the nucleus and the nucleolus during in vitro adipogenic differentiation of primary human adipose stem cells. We find that cell cycle arrest elicits a remodeling of nucleolar structure which correlates with a decrease in protein synthesis. Strikingly, triggering cytoskeletal rearrangements mimics the nucleolar remodeling observed during adipogenesis. Our results point to nucleolar remodeling as an active, mechano-regulated mechanism during adipogenic differentiation and demonstrate a key role of the actin cytoskeleton in defining nuclear and nucleolar architecture in differentiating human adipose stem cells.

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“…Correlative conventional and PALM (photoactivated localization microscopy) imaging enhances our capacity to analyse the mobility and time-averaged nanoscopic structural characteristics of locusspecific chromatin with single-molecule sensitivity (Mehra et al, 2022). Using point-spread-function engineering and deep-learningbased image analysis, we can now conduct live imaging of telomere diffusion (Naor et al, 2022).…”

Section: New Imaging Techniques Provide Insights Into Telomere Motionmentioning

confidence: 99%

Beyond tradition: exploring the non-canonical functions of telomeres in meiosis

Fernández-Álvarez

2023

Front. Cell Dev. Biol.

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The telomere bouquet is a specific chromosomal configuration that forms during meiosis at the zygotene stage, when telomeres cluster together at the nuclear envelope. This clustering allows cytoskeleton-induced movements to be transmitted to the chromosomes, thereby facilitating homologous chromosome search and pairing. However, loss of the bouquet results in more severe meiotic defects than can be attributed solely to recombination problems, suggesting that the bouquet’s full function remains elusive. Despite its transient nature and the challenges in performing in vivo analyses, information is emerging that points to a remarkable suite of non-canonical functions carried out by the bouquet. Here, we describe how new approaches in quantitative cell biology can contribute to establishing the molecular basis of the full function and plasticity of the bouquet, and thus generate a comprehensive picture of the telomeric control of meiosis.

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Quantifying cell-cycle-dependent chromatin dynamics during interphase by live 3D tracking

Cited by 4 publications

References 72 publications

Probing chromatin condensation dynamics in live cells using interferometric scattering correlation spectroscopy

Probing chromatin condensation dynamics in live cells using interferometric scattering correlation spectroscopy

Cytoskeletal remodeling defines nucleolar architecture during adipogenesis

Beyond tradition: exploring the non-canonical functions of telomeres in meiosis

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