Quantifying collective behavior in mammalian cells

Conrad, Jacinta C.

doi:10.1073/pnas.1205451109

Cited by 10 publications

(2 citation statements)

References 15 publications

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“…Similar relations between cell-cell adhesions (which affect density), motility and intercellular coordination occur in confluent monolayers; Inhibition of cadherin-mediated cell–cell adhesions increased general motility but reduced directionality, persistence and intercellular coordination for confluent monolayers of MCF10A cells [8] . Increased monolayer density is associated with decreased motility and increased intercellular coordination, experimentally [10] , [39] – [42] , theoretically [43] and known to exist in other systems as well, such as bacterial populations [44] .…”

Section: Discussionmentioning

confidence: 97%

Propagating Waves of Directionality and Coordination Orchestrate Collective Cell Migration

et al. 2014

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The ability of cells to coordinately migrate in groups is crucial to enable them to travel long distances during embryonic development, wound healing and tumorigenesis, but the fundamental mechanisms underlying intercellular coordination during collective cell migration remain elusive despite considerable research efforts. A novel analytical framework is introduced here to explicitly detect and quantify cell clusters that move coordinately in a monolayer. The analysis combines and associates vast amount of spatiotemporal data across multiple experiments into transparent quantitative measures to report the emergence of new modes of organized behavior during collective migration of tumor and epithelial cells in wound healing assays. First, we discovered the emergence of a wave of coordinated migration propagating backward from the wound front, which reflects formation of clusters of coordinately migrating cells that are generated further away from the wound edge and disintegrate close to the advancing front. This wave emerges in both normal and tumor cells, and is amplified by Met activation with hepatocyte growth factor/scatter factor. Second, Met activation was found to induce coinciding waves of cellular acceleration and stretching, which in turn trigger the emergence of a backward propagating wave of directional migration with about an hour phase lag. Assessments of the relations between the waves revealed that amplified coordinated migration is associated with the emergence of directional migration. Taken together, our data and simplified modeling-based assessments suggest that increased velocity leads to enhanced coordination: higher motility arises due to acceleration and stretching that seems to increase directionality by temporarily diminishing the velocity components orthogonal to the direction defined by the monolayer geometry. Spatial and temporal accumulation of directionality thus defines coordination. The findings offer new insight and suggest a basic cellular mechanism for long-term cell guidance and intercellular communication during collective cell migration.

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Section: Discussionmentioning

confidence: 97%

Propagating Waves of Directionality and Coordination Orchestrate Collective Cell Migration

et al. 2014

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“…The behaviour of mammalian cells, however, is commonly associated with a dependence of signalling pathways and time-dependent gene expression. 18 Thus, understanding intercellular communication of mammalian cells demands sensitive measurements of the cellular response to signalling molecules. In their investigation of the collective behaviour of fibroblasts, Sun et al first cultured fibroblast cells in the microfluidic channel with different cell densities before exposing the cells to flow of an ATP solution over the cells (Fig.…”

Section: Intercellular Population Communicationmentioning

confidence: 99%

Probing cell–cell communication with microfluidic devices

Guo

French

et al. 2013

Lab Chip

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Cell-cell communication plays an essential role in organismal development and functionality, and communication errors can lead to deleterious effects such as degenerative and autoimmune diseases. However, the intercellular communication network is extremely complex in multicellular organisms making isolation of the functional unit and study of basic mechanisms technically challenging. Despite the level of complexity and many challenges facing researchers in this area, recent development in microfluidic technology allows miniaturized and integrated devices to perform intercellular communication experiments on-chip. Microfluidics have many advantages, including the ability to accurately mimic the chemical, mechanical, and physical cellular microenvironment, precise spatial and temporal control, dynamic characterization, high throughput, scalability and reproducibility. In this Focus article, we highlight some of the recent works and advances in the application of microfluidics to the study of mammalian intercellular communication with particular emphasis on cell contact and soluble factor mediated communication. In addition, we provide some insights into the likely direction of the future developments in this field.

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Introduction of Social Influence Analysis

Xu¹,

Wu²

2020

SpringerBriefs in Optimization

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Quantifying collective behavior in mammalian cells

Cited by 10 publications

References 15 publications

Propagating Waves of Directionality and Coordination Orchestrate Collective Cell Migration

Propagating Waves of Directionality and Coordination Orchestrate Collective Cell Migration

Probing cell–cell communication with microfluidic devices

Introduction of Social Influence Analysis

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