Quantifying Cyanothece growth under DIC limitation

Inomura, Keisuke; Masuda, Takako; Eichner, Meri; Rabouille, Sophie; Zavřel, Tomáš; Červený, Jan; Vancová, Marie; Bernát, Gábor; Armin, Gabrielle; Claquin, Pascal; Kotabová, Eva; Stephan, Susanne; Suggett, David J.; Deutsch, Curtis; Prášil, Ondřej

doi:10.1016/j.csbj.2021.11.036

Cited by 2 publications

(3 citation statements)

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“…Overall, it was clear in the present experiment that algal mass is maximized by white light and phycobiliproteins by colored light. A sound scenario to obtain the most benefit from culturing these two cyanobacteria is to culture them in two steps: first, under white light of about 160 μmol/m 2 /sμmol/m 2 /s and with a supply of extra CO 2 , which can greatly enhance growth [ 93 ] in order to maximize biomass, and next, subjection to colored light in order to maximize phycobiliproteins.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Colored and White Light on Growth and Phycobiliproteins, Chlorophyll and Carotenoids Content of the Marine Cyanobacteria Phormidium sp. and Cyanothece sp. in Batch Cultures

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Antoniadis

2022

Life

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Two local marine cyanobacteria, Phormidium sp. and Cyanothece sp., were batch-cultured under 18–19.5 °C, at 40 ppt salinity, using white LED light of low (40 μmol photons/m2/s) and high (160 μmol/m2/s) intensity and, additionally, blue, green and red LED light. Yield was highest in high white light in both species (2.15 g dw/L in Phormidium, 1.47 g/L in Cyanothece), followed by green light (1.25 g/L) in Cyanothece and low white and green (1.26–1.33 g/L) in Phormidium. Green light maximized phycocyanin in Phormidium (0.45 mg/mL), while phycoerythrin was enhanced (0.17 mg/mL) by blue light and allophycocyanin by all colors (~0.80 mg/mL). All colors maximized phycocyanin in Cyanothece (~0.32 mg/mL), while phycoerythrin and allophycocyanin peaked under green light (~0.138 and 0.38 mg/mL, respectively). In Phormidium, maximization of chlorophyll-a (9.3 μg/mL) was induced by green light, while total carotenoids and b-carotene (3.05 and 0.89 μg/mL, respectively) by high white light. In Cyanothece, both white light intensities along with green maximized chlorophyll-a (~9 μg/mL) while high white light and green maximized total carotenoids (2.6–3.0 μg/mL). This study strongly indicates that these cyanobacteria can be cultured at the first stage under white light to accumulate sufficient biomass and, subsequently, under colored light for enhancing phycobiliproteins.

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Section: Discussionmentioning

confidence: 99%

The Effect of Colored and White Light on Growth and Phycobiliproteins, Chlorophyll and Carotenoids Content of the Marine Cyanobacteria Phormidium sp. and Cyanothece sp. in Batch Cultures

Hotos

Antoniadis

2022

Life

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“…We also recognize that there are other intracellular storage compounds such as cyanophycin, a major nitrogen storage compound, and/or phosphorus granules. However, their content is generally much smaller than that of storage carbohydrates [19] , [20] , [31] and can be neglected. Under exponential growth conditions, we observed much more dynamic diel changes in OD 735 in Crocosphaera (∼3 μm) compared to Cyanothece (∼3 μm).…”

Section: Resultsmentioning

confidence: 99%

“…The photobioreactor monitored and averaged the OD 735 data over every 1 or 5 min intervals and we normalized them to the OD 735 values recorded at 1 h after the onset of the light phase (1L). Based on our earlier studies [20] , [26] , [34] , we considered the increase in OD 735 during the light phase as a proxy for C accumulation by photosynthesis. In contrast, the decrease in OD 735 at the end of the light phase and also during the dark phase as a proxy for C consumption by respiration (see Discussion).…”

Section: Methodsmentioning

confidence: 99%