Many assessments of soil C have been restricted to the <2-mm fraction, but C has recently been identified in >2-mm fractions of forest mineral soils. Our objective was to determine the importance of the >2mm fraction to whole-soil C pools in Pacific Northwest old-growth coniferous forests. Seventy-nine pedons in 18 western Washington and Oregon forests were sampled to o depth of 100 cm. The <2-mm fraction was separated f'rom the >2-mm fractioo by airdrying, physicalty rrushing soil, and sieving; C was determined by Leco combustion. The >2-mm fraction contained up to 46% of the whole-soil C and averaged 23% for the seven forests that had C in that fraction. Following treatment with sodium hexametaphosphate to disaggregate soil material, up to 20% of whole-soil C remained in the >2-mm frsction Thus, the >2-mm fraction C appears to be in stable and unstable aggregates, as well as concretions. The whole-soil C in the surEsce 100 em of mineral soil ranged from 30 to 400 Mg C ha-'. Multiple regression analysis indicated this C pool was positively related to available water capacity, annual precipitation, and coarse woody debris (rZ = 0.63 to 0.66, n = 18 forests). Similar results were obtained with only the <2-mm soil C, which is the basis of previous regional evalmtions. This suggests consideration of the >2-mm fraction does not alter our understanding of the importance of dimate and soil texture as controls of soil C pools, but it does affect the quantificatiun of soil C pools lo many *ld-growth forests ie the Pocific Northwest.
SOIL IS A MAJOR POOL in regional budgets and the global cycles of C (Jobbagy and Jackson, 2000;Zinke and Stangenberger, 2000;Bernoux et al., 2002; Goodale et al., 2002). Soil C content varies considerably within landscapes and between regions. It is influenced by climate, soil texture, topography, vegetation, and land management (Homann et al., 1995; Jobbigy and Jackson, 2000;Parker et al., 2001). There is considerable uncertainty associated with estimates of soil C pools and their association with controlling variables, because of sampling and analytical difgculties, soil spatial variability, and methods of extrapolation to regions (Homann et al., 1995(Homann et al., , 1998(Homann et al., , 2001 Brejda et al., 2001).Most studies of soil C pools have measured only fine fractions of the soil, such as €2, <3, or <4 mm (Grigal and Ohmann, 1992;Canary et al., 2000;Parker et al., 2001; Brejda et al., 2001;Homann et al., 2001). Recent investigations of forests indicate the coarse fractions can the fine fraction would substantially underestimate the whole-soil C pool. For example, the <2-mm fraction contains only 63% of whole-soil C in a coastal Oregon forest (Cromack et al., 1999), 53 to 80% in three Australian forest sites (Bauhus et al., 2002), and 37% in one western Washington forest but 97% in another (Harrison et al., 2003).Some >2-mm C may be associated with charcoal or rocks (Bauhus et al., 2002;Harrison et al., 2003). Alternatively, it may be contained in aggregates that are not b...