Quantifying division of aortal smooth muscle cells in culture stimulated by 1,25(OH)2D3

Tukaj, Cecylia; Trzonkowski, Piotr; Kubasik-Juraniec, Jolanta; Myśliwski, Andrzej

doi:10.1016/j.jsbmb.2006.12.100

Cited by 10 publications

(11 citation statements)

References 11 publications

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“…At higher concentrations (10 e6 M), even the precursor 25(OH)D 3 promoted cell growth [10]. More recently, the promitogenic effect of 1,25(OH) 2 D 3 (at a concentration of 10 e8 M) was confirmed by Tukaj et al [43], who used a procedure based on quantitative, sequential halving of stably incorporated fluorescent dye (carboxyfluorescein diacetate succinimidyl ester (CFSE)) to assess proliferative activity in neonatal rat aortic VSMC. 1,25(OH) 2 D 3 has also been shown to regulate [ 3 H]-thymidine incorporation in both quiescent and nonquiescent neonatal rat aortic VSMC [9].…”

Section: Cell Proliferationmentioning

confidence: 87%

Vitamin D and the Cardiovascular System

et al. 2011

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Section: Cell Proliferationmentioning

confidence: 87%

Vitamin D and the Cardiovascular System

et al. 2011

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“…In principle, CFSE is retained by the primary cultured liver cells and is shared by the daughter cells at each division, resulting in multimodal flow cytometric CFSE histograms, with each cell generation clustering around half the fluorescence intensity of the previous one. 20 Cells were dyed with CFSE and incubated with 0, 10, or 100 ng/mL rhHPPCn for 24 hours as described in Patients and Methods. We measured the percentages of cells in the first 3 histograms: M1 (undivided), M2 (divided once), and M3 (divided twice).…”

Section: Effect Of Rhhppcn On Cell Growth and Proliferation In Vitromentioning

confidence: 99%

Isolation and functional identification of a novel human hepatic growth factor: Hepatopoietin Cn

Chu¹,

Zhang²,

Shi³

et al. 2008

Hepatology

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Hepatic stimulating substance (HSS) was first isolated from weanling rat liver in 1975 and found to stimulate hepatic DNA synthesis both in vitro and in vivo. Since then, mammalian and human HSS have been investigated for their potential to treat hepatic diseases. However, the essential nature in composition and structure of HSS remain puzzling because HSS has not been completely purified. Heating, ethanol precipitation, and ion-exchange chromatographies had been carried out to isolate the protein with specific stimulating activity from newborn calf liver, and [ 3 H]thymidine deoxyribose (TdR)/bromodeoxyuridine (BrdU) incorporation and carboxyfluorescein diacetate succinimidyl ester (CFSE)-based proliferation assay to determine the bioactivity in vitro and in vivo. We report the purification of a novel 30-kDa protein from a crude extract of calf liver HSS. This protein is a member of the leucine-rich acidic nuclear protein family (LANP) and has been named hepatopoietin Cn (HPPCn). Studies of partially hepatectomized (PH) mice show that levels of HPPCn messenger RNA (mRNA) increase after liver injury. Furthermore, the recombinant human protein (rhHPPCn) was shown to stimulate hepatic DNA synthesis and activate signaling pathways involved in hepatocyte proliferation in vitro and in vivo. Conclusion: HPPCn is a novel hepatic growth factor that plays a role in liver regeneration. (HEPATOLOGY 2008;47:986-995.)

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“…The cells formed highly abundant multilayer with flattened configuration. These data are in agreement with those reported in previous studies (Tukaj et al. 2000, 2007).…”

Section: Resultsmentioning

confidence: 99%

“…We used aortal SMCs derived from neonatal rats because it is known to produce much more elastin than that obtained from an adult one. The supra‐physiological (10 nM) concentration of 1,25(OH) 2 D 3 was chosen on the basis of results on before mentioned studies, in which the maximal proliferative effect was assessed (Tukaj et al. 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Calcitriol can exert a direct effect on vascular SMCs, which express vitamin D receptors (Merke et al 1987). Although calcitriol affects proliferation of vascular SMCs, its inhibitory or stimulatory effect is poorly elucidated and controversial (Koh et al 1988;Mitsuhashi et al 1991;Tukaj & Wrzołkowa 1997;Tukaj et al 2000Tukaj et al , 2007Somjen et al 2005). In the present study, morphological techniques were used to elucidate the role of calcitriol in proliferation, migration and formation of elastic fibres in the primary culture of aortal SMCs.…”

Section: Discussionmentioning

confidence: 99%

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Enhanced proliferation of aortal smooth muscle cells treated by 1,25(OH)₂D₃in vitro coincides with impaired formation of elastic fibres

Tukaj¹

2008

Int J Experimental Path

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Elastin is the major extracellular matrix component synthesized, secreted and deposited by vascular smooth muscle cells (SMCs) in the arterial media and thus plays an important role in vascular homeostasis. Results of our previous studies showed that 1alpha,25-dihydroxycholecalciferol [1,25(OH)(2)D(3)-calcitriol] accelerates proliferation of SMCs and modulates their growth in vitro. The aim of this study was to find ultrastructural support for the idea that 1,25(OH)(2)D(3)-calcitriol affects elastic fibre formation due to accelerated proliferation of aortal SMCs in vitro. SMCs exposed 10 days to supraphysiological concentration (10 nM) of calcitriol in primary culture were examined by fluorescence and transmission electron microscopy. Morphological studies revealed that calcitriol altered elastin maturation by favouring accumulation of immature rather than fully processed elastic fibres. A substantial decrease in the amorphous elastin deposition and abnormal accumulation of microfibrillar component, in thickened multilayer culture, were observed. These studies suggest that 1,25(OH)(2)D(3) affect formation of elastic fibres due to enhanced proliferation of SMCs in culture.

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Quantifying division of aortal smooth muscle cells in culture stimulated by 1,25(OH)2D3

Cited by 10 publications

References 11 publications

Vitamin D and the Cardiovascular System

Vitamin D and the Cardiovascular System

Isolation and functional identification of a novel human hepatic growth factor: Hepatopoietin Cn

Enhanced proliferation of aortal smooth muscle cells treated by 1,25(OH)₂D₃in vitro coincides with impaired formation of elastic fibres

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Quantifying division of aortal smooth muscle cells in culture stimulated by 1,25(OH)2D3

Cited by 10 publications

References 11 publications

Vitamin D and the Cardiovascular System

Vitamin D and the Cardiovascular System

Isolation and functional identification of a novel human hepatic growth factor: Hepatopoietin Cn

Enhanced proliferation of aortal smooth muscle cells treated by 1,25(OH)2D3in vitro coincides with impaired formation of elastic fibres

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Enhanced proliferation of aortal smooth muscle cells treated by 1,25(OH)₂D₃in vitro coincides with impaired formation of elastic fibres