Quantifying glucose permeability and enhanced light penetration in ex vivo human normal and cancerous esophagus tissues with optical coherence tomography

Zhao, Qingliang; Si, Junli; Guo, Zhou; Wei, Huajiang; Yang, Hongqin; Wu, Guo Yong; Xie, Shusen; Li, X.Y.; Guo, Xusheng; Zhong, Huiqing; Li, L.Q.

doi:10.1002/lapl.201010081

Cited by 59 publications

(46 citation statements)

References 62 publications

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“…On the other hand, a recent publication shows the results from a study of glucose diffusion permeability in normal and cancerous esophageal tissues. 20 Different studies to evaluate OCA concentration efficiency with tissue depth and improved contrast of optical coherence tomography or second-harmonic generation images at deeper tissue layers have also been reported. 3,5,27,28 Mathematical models have also been developed to describe OCA diffusion in biological tissues.…”

Section: Methodsmentioning

confidence: 99%

Diffusion characteristics of ethylene glycol in skeletal muscle

et al. 2014

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Part of the optical clearing study in biological tissues concerns the determination of the diffusion characteristics of water and optical clearing agents in the subject tissue. Such information is sufficient to characterize the time dependence of the optical clearing mechanisms-tissue dehydration and refractive index (RI) matching. We have used a simple method based on collimated optical transmittance measurements made from muscle samples under treatment with aqueous solutions containing different concentrations of ethylene glycol (EG), to determine the diffusion time values of water and EG in skeletal muscle. By representing the estimated mean diffusion time values from each treatment as a function of agent concentration in solution, we could identify the real diffusion times for water and agent. These values allowed for the calculation of the correspondent diffusion coefficients for those fluids. With these results, we have demonstrated that the dehydration mechanism is the one that dominates optical clearing in the first minute of treatment, while the RI matching takes over the optical clearing operations after that and remains for a longer time of treatment up to about 10 min, as we could see for EG and thin tissue samples of 0.5 mm.

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Section: Methodsmentioning

confidence: 99%

Diffusion characteristics of ethylene glycol in skeletal muscle

et al. 2014

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“…have been performed earlier with the following results: 40%-glucose permeation coe±-cient for skin is ð1:78 AE 0:04Þ Â 10 À6 cm/s, 26 for breast tissue it is ð5:76 AE 0:89Þ Â 10 À6 cm/s, 64 and for esophagus is ð1:74 AE 0:04Þ Â 10 À5 cm/s. 65 The glucose di®usion coe±cient in skeletal muscle and mammalian dermis was estimated as 8: Note: AE -standard deviation. a The permeation coe±cients have been calculated using the relation P ¼ D=l.…”

Section: Resultsmentioning

confidence: 99%

Quantification of glucose and glycerol diffusion in myocardium

Tuchina

Bashkatov

Genina

et al. 2015

J. Innov. Opt. Health Sci.

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The results on determination of glucose and glycerol di®usion coe±cients in myocardium tissue are presented. The method is based on the measurement and analysis of temporal dependence of tissue optical collimated transmittance under action of a hyperosmotic agent. This temporal tissue response is related to the rate of the agent and water di®usion in a tissue. The di®usion coe±cients for tissue°uid°uxes at glucose and glycerol application to the myocardium at 20 C have been estimated as ð4:75 AE 3:40Þ Â 10 À7 and ð7:71 AE 4:63Þ Â 10 À7 cm 2 /s, respectively.

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“…By placing an OCA in the interstitial space of the tissue and reducing the water content, the refractive index difference between the scatterers and the interstitial presented with results for the diffusion time for some OCAs such as dimethyl sulfoxide [17], glucose [18][19][20], mannitol [18], sucrose [21], glycerol [22], lactose and fructose [21] in different biological tissues and phantoms. A study that was recently published provided results for glucose diffusion permeability in normal and cancerous oesophageal tissues [23]. Other studies to evaluate the efficiency of OCA concentration with tissue depth and the improved contrast of OCT images at higher tissue depths have also been reported [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

The characteristic time of glucose diffusion measured for muscle tissue at optical clearing

et al. 2013

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The study of agent diffusion in biological tissues is very important to understand and characterize the optical clearing effects and mechanisms involved: tissue dehydration and refractive index matching. From measurements made to study the optical clearing, it is obvious that light scattering is reduced and that the optical properties of the tissue are controlled in the process. On the other hand, optical measurements do not allow direct determination of the diffusion properties of the agent in the tissue and some calculations are necessary to estimate those properties. This fact is imposed by the occurrence of two fluxes at optical clearing: water typically directed out of and agent directed into the tissue. When the water content in the immersion solution is approximately the same as the free water content of the tissue, a balance is established for water and the agent flux dominates. To prove this concept experimentally, we have measured the collimated transmittance of skeletal muscle samples under treatment with aqueous solutions containing different concentrations of glucose. After estimating the mean diffusion time values for each of the treatments we have represented those values as a function of glucose concentration in solution. Such a representation presents a maximum diffusion time for a water content in solution equal to the tissue free water content. Such a maximum represents the real diffusion time of glucose in the muscle and with this value we could calculate the corresponding diffusion coefficient.

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Quantifying glucose permeability and enhanced light penetration in ex vivo human normal and cancerous esophagus tissues with optical coherence tomography

Cited by 59 publications

References 62 publications

Diffusion characteristics of ethylene glycol in skeletal muscle

Diffusion characteristics of ethylene glycol in skeletal muscle

Quantification of glucose and glycerol diffusion in myocardium

The characteristic time of glucose diffusion measured for muscle tissue at optical clearing

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