Stable water isotopes are naturally occurring conservative tracers that can ‘fingerprint’ water sources and track ecohydrological fluxes across the critical zone (CZ). Parsimonious, tracer‐aided models allow effective quantification of the ecohydrological partitioning of rainfall into different water fluxes. We incorporated stable water isotopes into a one‐dimensional, tracer‐aided model (EcoIsoPlot) to follow the pathway of precipitation through the CZ at a lowland catchment—the long‐term experimental Demnitzer Millcreek Catchment (DMC), Germany—with contrasting vegetation covers (forest, agroforestry, grassland and arable). Precipitation (amount and δ2H), potential evapotranspiration (PET), leaf area index (LAI), air temperature and relative humidity were used as input data for modelling the growing season of 2021. The year had relatively average overall wetness, but a dry, cold spring with snowfall, and an exceptionally large summer storm event (~60 mm precipitation). Multi‐criteria calibration of the model was conducted using depth‐specific soil moisture and soil water δ2H measurements as targets. The novel incorporation of isotopes into model calibration constrained process representation of the estimated water balance with reasonable simulations and uncertainty bounds for water partitioning. Throughout the soil profile, soil moisture dynamics and stable water isotope variations were captured reasonably well. Green water fluxes (evapotranspiration) were highest at the forest site and blue water fluxes (groundwater recharge) highest at the grassland. Comparing simulations with estimated potential evapotranspiration (ET) and measured groundwater table fluctuations added further confidence to the modelling result. Overall, these may suggest a slight underestimation of ET and slight overestimation of recharge, though the results are similar to previous findings. Our study demonstrated the potential of stable water isotope data to enhance relatively simple, transferrable approaches to ecohydrological modelling of water fluxes in the CZ and to help improve model consistency. Such low‐parameterised tracer‐aided models have major potential for evidence‐based applications to aid management and help stakeholder communication.