Quantifying Micro-mechanical Properties of Soft Biological
                        Tissues with Scanning Acoustic Microscopy

Zhao, Xu; Wilkinson, S J; Akhtar, Riaz; Sherratt, Michael J.; Watson, Rachel; Derby, Brian

doi:10.1557/opl.2011.572

Cited by 5 publications

(3 citation statements)

References 14 publications

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“…In contrast to most other studies dealing with acoustic microscopy of soft tissues, 25-33 we were able to estimate c and Z from a single measurement. This allowed a direct pixel-by-pixel derivation of mechanical properties such as K and ρ. K is related to the Young’s modulus together with Poisson’s ratio (or shear modulus).…”

Section: Discussioncontrasting

confidence: 61%

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Material Properties of Human Ocular Tissue at 7-µm Resolution

et al. 2017

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Quantitative assessment of the material properties of ocular tissues can provide valuable information for investigating several ophthalmic diseases. Quantitative acoustic microscopy (QAM) offers a means of obtaining such information, but few QAM investigations have been conducted on human ocular tissue. We imaged the optic nerve (ON) and iridocorneal angle in 12-µm deparaffinized sections of the human eye using a custom-built acoustic microscope with a 250-MHz transducer (7-µm lateral resolution). The two-dimensional QAM maps of ultrasound attenuation (α), speed of sound ( c), acoustic impedance ( Z), bulk modulus ( K), and mass density (ρ) were generated. Scanned samples were then stained and imaged by light microscopy for comparison with QAM maps. The spatial resolution and contrast of scanning acoustic microscopy (SAM) maps were sufficient to resolve anatomic layers of the retina (Re); anatomic features in SAM maps corresponded to those seen by light microscopy. Significant variations of the acoustic parameters were found. For example, the sclera was 220 MPa stiffer than Re, choroid, and ON tissue. To the authors' knowledge, this is the first systematic study to assess c, Z, K, ρ, and α of human ocular tissue at the high ultrasound frequencies used in this study.

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Section: Discussioncontrasting

confidence: 61%

“…SAM was applied successfully to characterizing various soft tissues and disease states. 25-35 However, only a few studies have reported SAM measurements for ocular tissues. In a previous study by our group, we reported fine-resolution, acoustical-property maps of the murine Re at a 250-MHz center frequency.…”

Section: Introductionmentioning

confidence: 99%

Material Properties of Human Ocular Tissue at 7-µm Resolution

et al. 2017

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“…Active area of the transducer surface is defined by a small dot of gold (electrode), which is exactly opposite to the focusing surface. Plane acoustic waves generated by transducer travel through the disc and when they cross the spherical interface between the lens and the fluid are refracted towards the focus on the axis of the lens 20,21 . Focal length (f) of an AL immersed in water is similar to that in an optical system and can be calculated from equation 1-…”

Section: Introductionmentioning

confidence: 99%

Micro-Acoustic-Trap (µAT) for microparticle assembly in 3D

Vyas

Lemieux

Knecht

et al. 2019

Ultrasonics Sonochemistry

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With the current state of technology, use of Acoustic Tweezers (ATω) is limited to manipulation of single or few particles in single experimental setup. This article presents a state of the art system using Acoustic Lens (AL) as a Micro-Acoustic Trap (µAT) for microparticle assembly in 3D. In this investigation 2 micron sized polystyrene beads were used. Acoustic pressure generated by AL drives the particles towards the center of the acoustic focal plane, which leads to the formation of a free floating monolayer of latex particles. Transducer is driven at 89 MHz as both continuous wave (CW) and at mixed with pulsed (PL) frequency of 2Hz. The system was driven at drive amplitudes of 0.5 V, 1 V and 1.5 V. The most tightly packed monolayer of latex particles was observed at drive amplitude of 1.5 V. A loosely formed random close pack disc like structure was observed at 1 V whereas at 0.5 V drive amplitude particles were gently driven towards the center of the Acoustic Focal Plane (AFP) but no assemble was observed. This methodology was further extended for manipulating live Dictyostelium discoideum (Amoebas). At high drive amplitude of 2V, one can not only segregate non-adherent cells from adherent ones or move them to a region of interest but also can compress them and shrink their size. It was also observed that the cells having weaker cell membrane get distorted or membrane is rupture under a continuous stream of acoustic waves.

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A New Paradigm for Tissue Diagnostics: Tools and Techniques to Standardize Tissue Collection, Transport, and Fixation

2018

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Purpose of ReviewStudying and developing preanalytical tools and technologies for the purpose of obtaining high-quality samples for histological assays is a growing field. Currently, there does not exist a standard practice for collecting, fixing, and monitoring these precious samples. There has been some advancement in standardizing collection for the highest profile tumor types, such as breast, where HER2 testing drives therapeutic decisions. This review examines the area of tissue collection, transport, and monitoring of formalin diffusion and details a prototype system that could be used to help standardize tissue collection efforts.Recent FindingsWe have surveyed recent primary literature sources and conducted several site visits to understand the most error-prone processes in histology laboratories. This effort identified errors that resulted from sample collection techniques and subsequent transport delays from the operating room (OR) to the histology laboratories. We have therefore devised a prototype sample collection and transport concept. The system consists of a custom data logger and cold transport box and takes advantage of a novel cold + warm (named 2 + 2) fixation method.SummaryThis review highlights the beneficial aspects of standardizing tissue collection, fixation, and monitoring. In addition, a prototype system is introduced that could help standardize these processes and is compatible with use directly in the OR and from remote sites.

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Quantifying Micro-mechanical Properties of Soft Biological Tissues with Scanning Acoustic Microscopy

Cited by 5 publications

References 14 publications

Material Properties of Human Ocular Tissue at 7-µm Resolution

Material Properties of Human Ocular Tissue at 7-µm Resolution

Micro-Acoustic-Trap (µAT) for microparticle assembly in 3D

A New Paradigm for Tissue Diagnostics: Tools and Techniques to Standardize Tissue Collection, Transport, and Fixation

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