Quantifying microbial growth and carbon use efficiency in dry soil environments via <sup>18</sup>O water vapor equilibration

Canarini, Alberto; Wanek, Wolfgang; Watzka, Margarete; Sandén, Taru; Spiegel, Heide; Šantrůček, Jiří; Schnecker, Jörg

doi:10.1111/gcb.15168

Cited by 37 publications

(37 citation statements)

References 43 publications

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“…These comparisons showed a higher growth per respiration ratio at dry conditions in all sites. That is, at low moisture levels microbial communities allocated relatively more C for growth, suggesting a high CUE; consistent with recent assessments of steady‐state rates in an Austrian set of soils (Canarini et al., 2020), but contrasting with results from a temperate heath (de Nijs et al., 2019). In a laboratory experiment where soils were dried to different moisture contents, a higher CUE was found at low moisture levels and attributed to accumulation of osmolytes based on 13 C tracing (Herron et al., 2009).…”

Section: Discussionsupporting

confidence: 85%

Drought legacy affects microbial community trait distributions related to moisture along a savannah grassland precipitation gradient

et al. 2020

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Section: Discussionsupporting

confidence: 85%

Drought legacy affects microbial community trait distributions related to moisture along a savannah grassland precipitation gradient

et al. 2020

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“…Isotopic approaches (e.g. Canarini et al, 2020;Karlowsky, Augusti, Ingrisch, Hasibeder, et al, 2018) will be necessary to fully test such potential link between distinct responses of overall communities and functional differences among their constituents. Meta-omics approaches (Malik et al, 2019(Malik et al, , 2020 will further help to elucidate the taxa and traits driving these differences.…”

Section: Effect Of Management On Soil Microbial Community Resistance ...mentioning

confidence: 99%

Resistance–recovery trade‐off of soil microbial communities under altered rain regimes: An experimental test across European agroecosystems

Piton

Foulquier

Martínez‐García

et al. 2020

Journal of Applied Ecology

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With the increased occurrence of climate extremes, there is an urgent need to better understand how management strategies affect the capacity of the soil microbial community to maintain its ecosystem functions (e.g. nutrient cycling). To address this issue, intact monoliths were extracted from conventional and ecological managed grasslands in three countries across Europe and exposed under common air condition (temperature and moisture) to one of three altered rain regimes (dry, wet and intermittent wet/dry) as compared to a normal regime. Subsequently, we compared the resistance and recovery of the soil microbial biomass, potential enzyme activities and community composition. The microbial community composition differed with soil management and rain regimes. Soil microbial biomass increased from the wetter to the dryer rain regime, paralleling an increase of available carbon and nutrients, suggesting low sensitivity to soil moisture reduction but nutritional limitations of soil microbes. Conversely, enzyme activities decreased with all altered rain regimes. Resistance and recovery (considering absolute distance between normal and altered rain regime) of the microbial communities depended on soil management. Conventional‐intensive management showed higher resistance of two fundamental properties for nutrient cycling (i.e. bacterial biomass and extracellular enzyme activities) yet associated with more important changes in microbial community composition. This suggests an internal community reorganization promoting biomass and activity resistance. Conversely, under ecological management bacterial biomass and enzyme activities showed better recovery capacity, whereas no or very low recovery of these properties was observed under conventional management. These management effects were consistent across the three altered rain regimes investigated, indicating common factors controlling microbial communities’ response to different climate‐related stresses. Synthesis and applications. Our study provides experimental evidence for an important trade‐off for agroecosystem management between (a) stabilizing nutrient cycling potential during an altered rain regime period at the expense of very low recovery capacity and potential long‐term effect (conventional sites) and (b) promoting the capacity of the microbial community to recover its functional potential after the end of the stress (ecological sites). Thus, management based on ecologically sound principles may be the best option to sustain long‐term soil functioning under climate change.

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“…In this case, knowing the fungal DNA fraction is less important, but knowing how nutrient addition may change the relationship between cell carbon content and DNA production would help refine estimates of growth. It is also important to note that recent work has been completed to try and constrain the contribution of extracellular water to new DNA growth in soil (Qu et al, 2020) and to develop new vapor-based CUE measurements which disrupt the soil moisture regime less (Canarini et al, 2020). Implementing these methodological changes to the 18 O water method will effectively reduce the range of physiological parameters causing the observed CUE to deviate from its true value.…”

Section: Effect Of Common Experimental Factors On Apparent Microbial mentioning

confidence: 99%

Heavy and wet: The consequences of violating assumptions of measuring soil microbial growth efficiency using the 18O water method

2020

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Soils store more carbon than the biosphere and atmosphere combined, and the efficiency to which soil microorganisms allocate carbon to growth rather than respiration is increasingly considered a proxy for the soil capacity to store carbon. This carbon use efficiency (CUE) is measured via different methods, and more recently, the 18O-H2O method has been embraced as a significant improvement for measuring CUE of soil microbial communities. Based on extrapolating 18O incorporation into DNA to new biomass, this measurement makes various implicit assumptions about the microbial community at hand. Here we conducted a literature review to evaluate how viable these assumptions are and then developed a mathematical model to test how violating them affects estimates of the growth component of CUE in soil. We applied this model to previously collected data from two kinds of soil microbial communities. By changing one parameter at a time, we confirmed our previous observation that CUE was reduced by fungal removal. Our results also show that depending on the microbial community composition, there can be substantial discrepancies between estimated and true microbial growth. Of the numerous implicit assumptions that might be violated, not accounting for the contribution of sources of oxygen other than extracellular water to DNA leads to a consistent underestimation of CUE. We present a framework that allows researchers to evaluate how their experimental conditions may influence their 18O-H2O-based CUE measurements and suggest the parameters that need further constraining to more accurately quantify growth and CUE.

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Quantifying microbial growth and carbon use efficiency in dry soil environments via ¹⁸O water vapor equilibration

Cited by 37 publications

References 43 publications

Drought legacy affects microbial community trait distributions related to moisture along a savannah grassland precipitation gradient

Drought legacy affects microbial community trait distributions related to moisture along a savannah grassland precipitation gradient

Resistance–recovery trade‐off of soil microbial communities under altered rain regimes: An experimental test across European agroecosystems

Heavy and wet: The consequences of violating assumptions of measuring soil microbial growth efficiency using the 18O water method

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Quantifying microbial growth and carbon use efficiency in dry soil environments via 18O water vapor equilibration

Cited by 37 publications

References 43 publications

Drought legacy affects microbial community trait distributions related to moisture along a savannah grassland precipitation gradient

Drought legacy affects microbial community trait distributions related to moisture along a savannah grassland precipitation gradient

Resistance–recovery trade‐off of soil microbial communities under altered rain regimes: An experimental test across European agroecosystems

Heavy and wet: The consequences of violating assumptions of measuring soil microbial growth efficiency using the 18O water method

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Quantifying microbial growth and carbon use efficiency in dry soil environments via ¹⁸O water vapor equilibration