Quantifying Phase Behaviour in Model Food Composites Using 3D Confocal Laser Scanning Microscopy

Mhaske, Pranita; Kasapis, Stefan; Farahnaky, Asgar; Dokouhaki, Mina

doi:10.1007/s11483-021-09713-8

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…Covalent labeling of agarose by the small molecule fluorescein is a feasible strategy for in situ visualization of agarose. 40,41 Nevertheless, covalent labeling is laborious compared to noncovalent labeling by utilizing molecular probes. Besides, covalent labeling strategies are generally applicable to scenarios when agarose is supplemented to the target system as an exogenous component, which restricts the implementation of in situ visualizations of agarose in natural food materials.…”

Section: Discussionmentioning

confidence: 99%

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Discovery and characterization of a novel carbohydrate‐binding module: a favorable tool for investigating agarose

Mei,

Zhang,

Jiang

et al. 2023

J Sci Food Agric

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BACKGROUNDAgarose, mainly composed of 3,6‐anhydro‐α‐l‐galactopyranose (LA) and β‐d‐galactopyranose (G) units, is an important polysaccharide with wide applications in food, biomedical and bioengineering industries. Carbohydrate‐binding modules (CBMs) are favorable tools for the investigations of polysaccharides. Few agarose‐binding CBMs have been hitherto reported, and their binding specificity is unclear.RESULTSAn unknown domain with a predicted β‐sandwich fold was discovered from a β‐agarase of the marine bacterium Wenyingzhuangia fucanilytica CZ1127T. The expressed protein WfCBM101 could bind to agarose and exhibited relatively weak affinity for porphyran, with no affinity for the other seven examined polysaccharides. The protein binds to the tetrasaccharide (LA‐G)2, but not to the major tetrasaccharide contained in porphyran. The sequence novelty and well‐defined binding function of WfCBM101 shed light on a novel CBM family (CBM101). Furthermore, the feasibility of WfCBM101 for visualizing agarose in situ was confirmed.CONCLUSIONA novel CBM, WfCBM101, with a desired specificity for agarose was discovered and characterized, which represents a new CBM family. The CBM could be utilized as a promising tool for studies of agarose. © 2023 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

“…Covalent labeling of agarose by the small molecule fluorescein is a feasible strategy for in situ visualization of agarose 40,41 . Nevertheless, covalent labeling is laborious compared to noncovalent labeling by utilizing molecular probes.…”

Section: Discussionmentioning

confidence: 99%

Discovery and characterization of a novel carbohydrate‐binding module: a favorable tool for investigating agarose

Mei,

Zhang,

Jiang

et al. 2023

J Sci Food Agric

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“…Confocal microscopy, a non-contact optical measurement technique, finds wide applications in semiconductor inspection [1,2], biomedicine [3,4], and materials science [5][6][7] due to its exceptional axial resolution. The conventional technique for confocal microscopy measurement utilizes the principle of conjugate imaging with two pinholes.…”

Section: Introductionmentioning

confidence: 99%

Differential confocal effective measurement region extraction method based on dual measurement

Huang,

Yuan,

Chen

2024

Meas. Sci. Technol.

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Aiming at the problem that the existing differential confocal measurement (DCM) method lacks the ability to decouple the differential signals from over-range the depth data, which in turn leads to measurement errors, this study proposes a differential confocal effective measurement region extraction method based on dual measurement (D-DCM). The first measurement is a double blur depth measurement, which is a coarse measurement, and the initial acquisition of the depth map is realized by applying a known Gaussian kernel blurring to the focal plane image and calculating the edge gradient ratios between the original image and the blurred image. This depth map is used to determine whether the depth of the measured sample in the field of view is within the differential confocal range and to segment it. The second measurement is a DCM, which is a fine measurement. The effective measurement area of the segmented image is second measured with high precision depth measurement. It is shown by single and multiple step heights experiments that the proposed D-DCM method can effectively solve the problem of decoupling the differential signal from the axial depth in differential confocal.

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Multitechnique approaches for characterising phase behaviour in food biopolymer systems

Mhaske,

Farahnaky,

Dokouhaki

et al. 2024

Int J of Food Sci Tech

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SummaryUnderstanding the phase behaviour of biopolymer composites is vital for various technological applications, such as developing functional foods, nutraceuticals, encapsulation, isolation and fractionation of enzymes and proteins. The growing consumer demand for healthier food products pressures the food and nutraceutical industries to innovate while maintaining traditional texture and nutritional quality. This review critically examines various analytical techniques, including spectroscopic, microscopic, calorimetric, rheological, viscometry, and diffraction methods, used to study phase behaviour. Each technique provides unique insights into phase behaviour mechanisms at micro and macroscopic levels, with respective advantages and limitations discussed. The review advocates for a multi‐technique approach for a comprehensive understanding of phase behaviour and highlights the development of in situ probing techniques with minimal sample pre‐processing as a promising advancement. These innovations are expected to significantly enhance the practical applications of these techniques in the food industry, meeting current consumer demands without compromising traditional qualities.

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Quantifying Phase Behaviour in Model Food Composites Using 3D Confocal Laser Scanning Microscopy

Cited by 4 publications

References 12 publications

Discovery and characterization of a novel carbohydrate‐binding module: a favorable tool for investigating agarose

Discovery and characterization of a novel carbohydrate‐binding module: a favorable tool for investigating agarose

Differential confocal effective measurement region extraction method based on dual measurement

Multitechnique approaches for characterising phase behaviour in food biopolymer systems

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