Quantifying the effects of contact duration, loading rate, and approach velocity on P-selectin–PSGL-1 interactions using AFM

Lü, Shouqin; Ye, Zhiyi; Zhu, Cheng; Long, Mian

doi:10.1016/j.polymer.2005.11.095

Cited by 27 publications

(39 citation statements)

References 46 publications

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“…Two parameters are used to quantify the effect: one is the bond rupture force or bond strength, and another is bond lifetime. The bond rupture force depends on the rate of force application or force loading rate [28,29,[37][38][39][40][41][42], and other extrinsic physical parameters [43]. Bond lifetime is governed by external forces, as proposed by Bell [27] and Dembo [26],…”

Section: Theoretical Framework For Receptor-ligand Kineticsmentioning

confidence: 99%

“…From then on, many experimental assays have been developed by coordinating the biological experiments and mechanical measurements. These include micropipette aspiration [25,31,36,51], optical tweezers [37,52,53], biological force probes [45,[54][55][56], atomic force microscopy (AFM) [38,39,43,[57][58][59][60][61], flow chamber [62][63][64][65], microcantilever needle [28], centrifugation [66], rosetting [67], cone-plate viscometer [68,69], surface force apparatus [70,71], and fluorescence recovery after photobleaching (FRAP) [72,73]. Here, two assays of micropipette aspiration and atomic force microscopy are exemplified to demonstrate how they work.…”

Section: Experimental Approaches For Quantifying 2d Kinetics and Forcmentioning

confidence: 99%

“…c Force-displacement curves illustrating two working modes of bond lifetime and rupture force then reconstituted by vesicle fusion in a polyethylenimine (PEI) polymer-supported lipid bilayer onto a mica or glass surface before use (Fig. 3b) [38,39,43,[57][58][59][60][61]. The ligandor receptor-reconstituted lipid bilayer is placed on the AFM stage, which is repeatedly driven by a piezoelectric translator (PZT) to approach the receptor-or ligand-coated cantilever tip, to make contact to allow reversible bond formation and dissociation, and to retract away to allow observation of the adhesion event and measurement of lifetime or rupture force, if any (Fig.…”

Section: Experimental Approaches For Quantifying 2d Kinetics and Forcmentioning

confidence: 99%

“…Measured P a vs. t data is fitted to the model (Eq. 3) to obtain the kinetic parameters (k 0 r and A c m r m l K 0 a ) [43]. Bond lifetime data, <τ > vs. f, are measured to test the forced dissociation hypotheses (Eq.…”

Section: Experimental Approaches For Quantifying 2d Kinetics and Forcmentioning

confidence: 99%

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Mechanokinetics of receptor–ligand interactions in cell adhesion

Lü

Zhang

et al. 2015

Acta Mech Sin

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Receptor-ligand interactions in blood flow are crucial to initiate such biological processes as inflammatory cascade, platelet thrombosis, as well as tumor metastasis. To mediate cell adhesion, the interacting receptors and ligands must be anchored onto two apposing surfaces of two cells or a cell and a substratum, i.e., two-dimensional (2D) binding, which is different from the binding of a soluble ligand in fluid phase to a receptor, i.e., three-dimensional (3D) binding. While numerous works have been focused on 3D kinetics of receptor-ligand interactions in the immune system, 2D kinetics and its regulations have been less understood, since no theoretical framework or experimental assays were established until 1993. Not only does the molecular structure dominate 2D binding kinetics, but the shear force in blood flow also regulates cell adhesion mediated by interacting receptors and ligands. Here, we provide an overview of current progress in 2D binding and regulations, mainly from our group. Relevant issues of theoretical frameworks, experimental measurements, kinetic rates and binding affinities, and force regulations are discussed.Keywords Receptor-ligand interactions · Selectins · β 2 integrins · 2D binding kinetics B Mian Long

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Section: Theoretical Framework For Receptor-ligand Kineticsmentioning

confidence: 99%

Section: Experimental Approaches For Quantifying 2d Kinetics and Forcmentioning

confidence: 99%

Section: Experimental Approaches For Quantifying 2d Kinetics and Forcmentioning

confidence: 99%

Section: Experimental Approaches For Quantifying 2d Kinetics and Forcmentioning

confidence: 99%

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Mechanokinetics of receptor–ligand interactions in cell adhesion

Lü

Zhang

et al. 2015

Acta Mech Sin

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“…2D dissociation kinetics (Hammer and Lauffenburger 1987;Alon et al 1995;Chen et al 1997) and forced bond rupture (Florin et al 1994;Dammer et al 1996;Tees et al 2001) as well as their regulating factors (Evans et al 2001;Levin et al 2001;Huang et al 2004;Marshall et al 2005;Wu et al 2007) have been investigated theoretically and experimentally using various approaches or assays, e.g., flow chamber (Kaplanski et al 1993;Alon et al 1995;Finger et al 1996;Yago et al 2007;Paschall et al 2008), biomembrane force probe (BFP) (Evans et al 2001;Evans and Ritchie 1997), atom force microscopy (AFM) (Fritz et al 1998;Merkel et al 1999;Marshall et al 2005;Lü et al 2006), micropipette aspiration (Chesla et al 1998;Long et al 2001;Shao and Xu 2002), optical tweezers (Kulin et al 2002;Rinko et al 2004), fluorescence recovery after photobleaching (FRAP) (Dustin et al 1996;Tolentino et al 2008). Only a few works, however, were focused on quantifying 2D association kinetics mainly due to theoretical and technical limitations.…”

Section: Introductionmentioning

confidence: 99%

Surface-bound selectin–ligand binding is regulated by carrier diffusion

et al. 2009

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Two-dimensional (2D) kinetics of receptorligand interactions governs cell adhesion in many biological processes. While the dissociation kinetics of receptorligand bond is extensively investigated, the association kinetics has much less been quantified. Recently receptorligand interactions between two surfaces were investigated using a thermal fluctuation assay upon biomembrane force probe technique (Chen et al. in Biophys J 94:694-701, 2008). The regulating factors on association kinetics, however, are not well characterized. Here we developed an alternative thermal fluctuation assay using optical trap technique, which enables to visualize consecutive bindingunbinding transition and to quantify the impact of microbead diffusion on receptor-ligand binding. Three selectin constructs (sLs, sPs, and PLE) and their ligand P-selectin glycoprotein ligand 1 were used to conduct the measurements. It was indicated that bond formation was reduced by enhancing the diffusivity of selectin-coupled carrier, suggesting that carrier diffusion is crucial to determine receptor-ligand binding. It was also found that 2D forward rate predicted upon first-order kinetics was in the order of sPs [ sLs [ PLE and bond formation was history-dependent. These results further the understandings in regulating association kinetics of surface-bound receptor-ligand interactions.

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Scanning Probe Microscopy for the Study of Interactions Involving Glycoproteins and Carbohydrates

Tan

2015

Carbohydrate Nanotechnology

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Quantifying the effects of contact duration, loading rate, and approach velocity on P-selectin–PSGL-1 interactions using AFM

Cited by 27 publications

References 46 publications

Mechanokinetics of receptor–ligand interactions in cell adhesion

Mechanokinetics of receptor–ligand interactions in cell adhesion

Surface-bound selectin–ligand binding is regulated by carrier diffusion

Scanning Probe Microscopy for the Study of Interactions Involving Glycoproteins and Carbohydrates

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