2017
DOI: 10.1038/srep40628
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Quantifying the relative contributions of different solute carriers to aggregate substrate transport

Abstract: Determining the contributions of different transporter species to overall cellular transport is fundamental for understanding the physiological regulation of solutes. We calculated the relative activities of Solute Carrier (SLC) transporters using the Michaelis-Menten equation and global fitting to estimate the normalized maximum transport rate for each transporter (Vmax). Data input were the normalized measured uptake of the essential neutral amino acid (AA) L-leucine (Leu) from concentration-dependence assay… Show more

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Cited by 12 publications
(24 citation statements)
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“…Cloning of hLAT4 into pSDeasy vector and FLAG‐tagging the construct was done as described previously (Taslimifar et al . ). To mutagenize serines 274, 278 and 297 to either alanine (A) or glutamate (E), the QuikChange Lightning Site‐Directed mutagenesis kit was used (Cat.…”
Section: Methodsmentioning
confidence: 97%
See 4 more Smart Citations
“…Cloning of hLAT4 into pSDeasy vector and FLAG‐tagging the construct was done as described previously (Taslimifar et al . ). To mutagenize serines 274, 278 and 297 to either alanine (A) or glutamate (E), the QuikChange Lightning Site‐Directed mutagenesis kit was used (Cat.…”
Section: Methodsmentioning
confidence: 97%
“…IMP XL FM, RRID:XEP_XLFM, Xenopus Express, Vernassal, France) and buffer preparation was done as previously described (Taslimifar et al . ). Briefly, stage IV oocytes were selected and treated with collagenase A.…”
Section: Methodsmentioning
confidence: 97%
See 3 more Smart Citations